Hanseniaspora uvarum Attracts Drosophila suzukii (Diptera: Drosophilidae) With High Specificity

Abstract

Since the early phase of the intercontinental dispersal of Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), fermentation baits have been used for monitoring. Self-made lures and commercial products are often based on wine and vinegar. From an ecological perspective, the formulation of these baits is expected to target especially vinegar flies associated with overripe fruit, such as Drosophila melanogaster (Meigen) (Diptera: Drosophilidae). Hanseniaspora uvarum (Niehaus) (Ascomycota: Saccharomyceta) is a yeast closely associated with D. suzukii and fruit, and furthermore attractive to the flies. Based on this relation, H. uvarum might represent a suitable substrate for the development of lures that are more specific than vinegar and wine. In the field, we therefore, compared H. uvarum to a commercial bait that was based on vinegar and wine with respect to the number of trapped D. suzukii relative to other drosophilids and arthropods. Trap captures were higher with the commercial bait but specificity for D. suzukii was greater with H. uvarum. Moreover, H. uvarum headspace extracts, as well as a synthetic blend of H. uvarum volatiles, were assayed for attraction of D suzukii in a wind tunnel and in the field. Headspace extracts and the synthetic blend induced strong upwind flight in the wind tunnel and confirmed attraction to H. uvarum volatiles. Furthermore, baited with H. uvarum headspace extract and a drowning solution of aqueous acetic acid and ethanol, 74% of field captured arthropods were D. suzukii. Our findings suggest that synthetic yeast headspace formulations might advance the development of more selective monitoring traps with reduced by-catch.

Heterologous expression and functional characterization of Drosophila suzukii OR69a transcript variants unveiled response to kairomones and to a candidate pheromone

“…4). Interestingly, parallel experiments conducted with headspaces of Hanseniaspora uvarum that were already available in our labs and on use for different projects (Mori et al 2017;Kleman et al 2022) revealed activation of DsuzOR69a subunits in proximity of compounds released at different retention times (Fig. 5, Online Resource 5).…”

A Versatile Toolset for Genetic Manipulation of the Wine Yeast Hanseniaspora uvarum

“…Hanseniaspora uvarum can also be employed in another type of biological control to fight Drosophila suzukii, which already causes fruit harvest losses in Southern Europe, especially of grapes and cherries [15]. The strong attraction of the fruit flies to H. uvarum, presumably due to the efficient production of ester compounds like ethyl acetate, is thus employed to build traps for efficient pest management [16,17]. Interestingly, but not yet applied in oenology or medicine, H. uvarum also produces a killer toxin with activity against Candida species [18].…”