<i>Hansenula polymorpha</i>Aat2p is targeted to peroxisomes via a novel Pex20p‐dependent pathway

Thomas, Ann; Krikken, Arjen M; Boer, Rinse de; Williams, Chris

doi:10.1002/1873-3468.13168

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…Immuno-EM was performed as described previously (Thomas et al, 2018). Labeling of HA was performed using monoclonal antibodies (Sigma-Aldrich H9658; 1:100 dilution) followed by goat anti-mouse antibodies conjugated to 6 nm gold (Aurion, The Netherlands; 1:20 dilution).…”

Section: Electron Microscopymentioning

confidence: 99%

Pex24 and Pex32 are required to tether peroxisomes to the ER for organelle biogenesis, positioning and segregation in yeast

Boer

Krikken

et al. 2020

Journal of Cell Science

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The yeast Hansenula polymorpha contains four members of the Pex23 family of peroxins, which characteristically contain a DysF domain. Here we show that all four H. polymorpha Pex23 family proteins localize to the endoplasmic reticulum (ER). Pex24 and Pex32, but not Pex23 and Pex29, predominantly accumulate at peroxisome–ER contacts. Upon deletion of PEX24 or PEX32 – and to a much lesser extent, of PEX23 or PEX29 – peroxisome–ER contacts are lost, concomitant with defects in peroxisomal matrix protein import, membrane growth, and organelle proliferation, positioning and segregation. These defects are suppressed by the introduction of an artificial peroxisome–ER tether, indicating that Pex24 and Pex32 contribute to tethering of peroxisomes to the ER. Accumulation of Pex32 at these contact sites is lost in cells lacking the peroxisomal membrane protein Pex11, in conjunction with disruption of the contacts. This indicates that Pex11 contributes to Pex32-dependent peroxisome–ER contact formation. The absence of Pex32 has no major effect on pre-peroxisomal vesicles that occur in pex3 atg1 deletion cells.

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Section: Electron Microscopymentioning

confidence: 99%

Pex24 and Pex32 are required to tether peroxisomes to the ER for organelle biogenesis, positioning and segregation in yeast

Boer

Krikken

et al. 2020

Journal of Cell Science

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show abstract

“…Immuno-EM was performed as described previously (Thomas et al, 2018). Labeling of HA was performed using monoclonal antibodies (Sigma-Aldrich H9658) followed by goatanti-mouse antibodies conjugated to 6 nm gold (Aurion, the Netherlands).…”

Section: Electron Microscopymentioning

confidence: 99%

Pex24 and Pex32 tether peroxisomes to the ER for organelle biogenesis, positioning and segregation

Wu¹,

Boer²,

Krikken³

et al. 2020

Preprint

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Two Hansenula polymorpha ER proteins, Pex24 and Pex32, are tethers at peroxisome-ER contacts and function together with the peroxisomal protein Pex11. Their absence disturbs these contacts leading to multiple peroxisomal defects, which can be restored by an artificial tether. AbstractWe analyzed all four Pex23 family proteins of the yeast Hansenula polymorpha, which localize to the ER. Of these Pex24 and Pex32, but not Pex23 and Pex29, accumulate at peroxisome-ER contacts, where they are important for normal peroxisome biogenesis and proliferation and contribute to organelle positioning and segregation.Upon deletion of PEX24 and PEX32 -and to a lesser extent of PEX23 and PEX29peroxisome-ER contacts are disrupted, concomitant with peroxisomal defects. These defects are suppressed upon introduction of an artificial peroxisome-ER tether.

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“…Immuno-EM was performed as described previously ( Thomas et al, 2018 ). Labeling of HA was performed using monoclonal antibodies (Sigma-Aldrich, H9658) followed by goat-anti-mouse antibodies conjugated to 6 nm gold (Aurion).…”

Section: Methodsmentioning

confidence: 99%

Peroxisome retention involves Inp1-dependent peroxisome–plasma membrane contact sites in yeast

Krikken

Boer

et al. 2020

Journal of Cell Biology

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Retention of peroxisomes in yeast mother cells requires Inp1, which is recruited to the organelle by the peroxisomal membrane protein Pex3. Here we show that Hansenula polymorpha Inp1 associates peroxisomes to the plasma membrane. Peroxisome–plasma membrane contact sites disappear upon deletion of INP1 but increase upon INP1 overexpression. Analysis of truncated Inp1 variants showed that the C terminus is important for association to the peroxisome, while a stretch of conserved positive charges and a central pleckstrin homology-like domain are important for plasma membrane binding. In cells of a PEX3 deletion, strain Inp1-GFP localizes to the plasma membrane, concentrated in patches near the bud neck and in the cortex of nascent buds. Upon disruption of the actin cytoskeleton by treatment of the cells with latrunculin A, Inp1-GFP became cytosolic, indicating that Inp1 localization is dependent on the presence of an intact actin cytoskeleton.

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Hansenula polymorphaAat2p is targeted to peroxisomes via a novel Pex20p‐dependent pathway

Cited by 6 publications

References 45 publications

Pex24 and Pex32 are required to tether peroxisomes to the ER for organelle biogenesis, positioning and segregation in yeast

Pex24 and Pex32 are required to tether peroxisomes to the ER for organelle biogenesis, positioning and segregation in yeast

Pex24 and Pex32 tether peroxisomes to the ER for organelle biogenesis, positioning and segregation

Peroxisome retention involves Inp1-dependent peroxisome–plasma membrane contact sites in yeast

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