<i>Hedysarum</i>— a new susceptible host for<i>Phytophthora medicaginis</i>

Southwell, R. J.; Crocker, G. J.

doi:10.1071/ap05029

Cited by 4 publications

(2 citation statements)

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“…In chickpea, there is no evidence for race-based resistance, but P. medicaginis can affect other hosts such as lucerne and annual medics (Knights et al 2008;Southwell and Crocker 2005). Many of the described Phytophthora pathosystems are characterised by both partial and racespecific resistance for PRR; for example in soybean (Glycine max L.), PRR is caused by specific races of P. sojae A.…”

Section: Introductionmentioning

confidence: 99%

Mapping resistance to Phytophthora root rot identifies independent loci from cultivated (Cicer arietinum L.) and wild (Cicer echinospermum P.H. Davis) chickpea

et al. 2018

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Section: Introductionmentioning

confidence: 99%

Mapping resistance to Phytophthora root rot identifies independent loci from cultivated (Cicer arietinum L.) and wild (Cicer echinospermum P.H. Davis) chickpea

et al. 2018

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“…In an unpublished (Sean Bithell, personal communication) inoculated glasshouse experiment for the testing of phytophthora isolates on the parental chickpea genotypes of the two RIL mapping populations, the isolate 1129-1 showed a pathogen–host interaction profile (in terms of plant mortality) similar to that of a mix of 10 P. medicaginis isolates used in field disease experiments and trials within the breeding program. For this reason, the isolate 1129-1 (DAR 66075) collected from Yetman, New South Wales, Australia [19], which has been reported to infect chickpea and Hedysarum spp., was used in this study. Initial pilot-scale testing confirmed the compatible interactions of the chickpea genotypes Rupali, Genesis 114 and breeding line 04067-81-2-1-1 with the P. medicaginis isolate 1129-1.…”

Section: Discussionmentioning

confidence: 99%

A hydroponics based high throughput screening system for Phytophthora root rot resistance in chickpea (Cicer arietinum L.)

2019

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Background Phytophthora root rot (PRR) caused by P. medicaginis is a major soil borne disease in chickpea growing regions of Australia. Sources of resistance have been identified in both cultivated and wild Cicer species. However, the molecular basis underlying PRR resistance is not known. Current phenotyping methods rely on mycelium slurry or oospore inoculum. Sensitive and reliable methods are desirable to study variation for PRR resistance in chickpea and allow for a controlled inoculation process to better capture early defence responses following PRR infection. Results In this study, a procedure for P. medicaginis zoospore production was standardized and used as the inoculum to develop a hydroponics based in planta infection method to screen chickpea genotypes with established levels of PRR resistance. The efficiency of the system was both qualitatively validated based on observation of characteristic PRR symptom development, and quantitatively validated based on the amount of pathogen DNA in roots. This system was scaled up to screen two biparental mapping populations previously developed for PRR studies. For each of the screenings, plant survival time was measured after inoculation and used to derive Kaplan–Meier estimates of plant survival (KME-survival). KME-survival and canker length were then selected as phenotypic traits associated with PRR resistance. Genetic analysis of these traits was conducted which identified quantitative trait loci (QTL). Additionally, these hydroponic traits and a set of previously published plant survival traits obtained from multiple PRR field experiments were combined in a model-based correlation analysis. The results suggest that the underlying genetic basis for plant survival during PRR infection within hydroponics and field disease environments is linked. The QTL QRBprrkms03 and QRBprrck03 on chromosome 4 identified for the traits KME-survival and canker length, respectively, correspond to the same region reported for PRR resistance in a field disease experiment. Conclusion A hydroponics based screening system will facilitate reliable and rapid screening in both small- and large-scale experiments to study PRR disease in chickpea. It can be applied in chickpea breeding programs to screen for PRR resistance and classify the virulence of new and existing P. medicaginis isolates.

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Resistance to Phytophthora medicaginis Hansen and Maxwell in wild Cicer species and its use in breeding root rot resistant chickpea (Cicer arietinum L.)

Knights

Southwell

Schwinghamer

et al. 2008

Aust. J. Agric. Res.

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Phytophthora root rot caused by Phytophthora medicaginis is a major disease of chickpea in Australia. Only partial resistance, derived from chickpea, is available in Australian cultivars. Five wild Cicer species were compared with chickpea cv. Jimbour (moderately resistant) in a field experiment. The proportions of accessions with significantly lower (P < 0.05) disease scores, where lower scores equate to higher resistance, were 9/9 for C. echinospermum, 9/21 for C. bijugum, 1/4 for C. judaicum, 1/29 for C. reticulatum, and 0/3 for C. pinnatifidum. The resistance of C. echinospermum (7/7 accessions) but not the other Cicer species was reproduced in a greenhouse test. Nine out of 30 chickpea × C. echinospermum-derived lines were as resistant as the C. echinospermum parents in a separate greenhouse experiment. C. echinospermum appears to be the best of the sources we examined for breeding chickpea cultivars resistant to P. medicaginis.

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Hedysarum— a new susceptible host forPhytophthora medicaginis

Cited by 4 publications

References 6 publications

Mapping resistance to Phytophthora root rot identifies independent loci from cultivated (Cicer arietinum L.) and wild (Cicer echinospermum P.H. Davis) chickpea

Mapping resistance to Phytophthora root rot identifies independent loci from cultivated (Cicer arietinum L.) and wild (Cicer echinospermum P.H. Davis) chickpea

A hydroponics based high throughput screening system for Phytophthora root rot resistance in chickpea (Cicer arietinum L.)

Resistance to Phytophthora medicaginis Hansen and Maxwell in wild Cicer species and its use in breeding root rot resistant chickpea (Cicer arietinum L.)

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