<i>Helicobacter pylori cagA</i>polymorphism and gastric inflammation: An international comparison between Japanese and Brazilian patients

Takamura, Akemi; Ito, Masanori; Imagawa, Shinobu; Takata, Shunsuke; Tanaka, Shinji; Teixeira, Cláudio Rolim; Kamada, Takenobu; Haruma, Ken; Chayama, Kazuaki

doi:10.3109/00365521.2011.598549

Cited by 2 publications

(3 citation statements)

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“…Takamura et al ( 26 ) employed PCR-RFLP for genotyping the H. pylori cagA gene isolated from paraffin-embedded sections of gastric cancer. The study populations were Brazilian and Japanese patients.…”

Section: Discussionmentioning

confidence: 99%

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Genotyping of the Helicobacter pylori cagA Gene Isolated From Gastric Biopsies in Shiraz, Southern Iran: A PCR-RFLP and Sequence Analysis Approach

Moaddeb

Fattahi

Firouzi

et al. 2016

Jundishapur J Microbiol

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BackgroundCytotoxin-associated gene A (cagA) is an important virulence factor in the pathogenesis of Helicobacter pylori.ObjectivesThe aim of this study was to genotype the H. pylori cagA gene isolated from antral biopsies of patients with stomach symptoms, using a PCR-restriction fragment length polymorphism (PCR-RFLP) analysis.Patients and MethodsA total of 161 gastric biopsies were collected from patients with stomach symptoms. After isolation of H. pylori from the biopsy culture, the cagA gene was assessed using PCR. The PCR products were then digested by the HinfI restriction endonuclease enzyme. A sample of each genotype was also subjected to direct sequencing for further analysis.ResultsFrom 161 antral biopsies, 61 (37.9%) were positive for H. pylori in culture. Overall, 24 cagA-positives were detected in the isolates. RFLP indicated three different genotypes (I, II, and III) of cagA with a frequency of 62.5%, 25%, and 12.5% among the isolates, respectively. Genotypes I and II of cagA were predominant in patients who had gastritis. However, genotype III was found in three patients with duodenitis and duodenal ulcers. Alignment of the nucleotide sequences of the three isolated genotypes, with H. pylori 26695 as a reference strain, revealed 12 inserted nucleotides in genotype III. When the sequence of genotype III was aligned with 15 additional H. pylori strains available in GenBank, the same inserted nucleotides were detected in six of them.ConclusionsUsing the PCR-RFLP method, three distinctive H. pylori cagA genotypes were detected in antral biopsies. Genotype I, which was predominant among the isolates, was significantly associated with gastritis. However, the data showed that cagA genotype III may play a role in duodenitis and duodenal ulcers in patients infected with H. pylori.

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Section: Discussionmentioning

confidence: 99%

“…The study populations were Brazilian and Japanese patients. Analysis of PCR-RFLP for the cagA gene showed that the prevalence of the east Asia subtype was significantly higher in the Japanese subjects than in the Brazilians ( 26 ), and the authors concluded that this genotype contributes to the progression of gastritis in the region.…”

Section: Discussionmentioning

confidence: 99%

Genotyping of the Helicobacter pylori cagA Gene Isolated From Gastric Biopsies in Shiraz, Southern Iran: A PCR-RFLP and Sequence Analysis Approach

Moaddeb

Fattahi

Firouzi

et al. 2016

Jundishapur J Microbiol

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show abstract

“…It is well known that the incidence of gastric cancer is high in East-Asia compared with other countries, even where the prevalence of HP infection is comparable. In a study from Japan/Brazil, Takamura et al [6] studied polymorphism of cagA, and found differences that they speculate could be of importance for the high incidence of gastric cancer in East-Asia and particularly Japan. An Indian study suggests that sequential treatment could be of benefit in eradicating HP [7].…”

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confidence: 96%

This month in Scandinavian Journal of Gastroenterology

2011

Scandinavian Journal of Gastroenterology

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Helicobacter pylori cagApolymorphism and gastric inflammation: An international comparison between Japanese and Brazilian patients

Abstract: Infection with H. pylori possessing the East Asian cagA gene contributes to the progression of gastritis.

Cited by 2 publications

References 21 publications

Genotyping of the Helicobacter pylori cagA Gene Isolated From Gastric Biopsies in Shiraz, Southern Iran: A PCR-RFLP and Sequence Analysis Approach

Genotyping of the Helicobacter pylori cagA Gene Isolated From Gastric Biopsies in Shiraz, Southern Iran: A PCR-RFLP and Sequence Analysis Approach

This month in Scandinavian Journal of Gastroenterology

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