<i>Helicobacter pylori</i> in the oral cavity is associated with gastroesophageal disease

Morales-Espinosa, Rosario; Fernández-Presas, Ana María; González-Valencia, Gerardo; Flores‐Hernández, Sergio; Delgado, Gabriela; Méndez-Sánchez, José Luis; Sanchez-Quezada, E.; Muñoz-Pérez, Leopoldo; León-Aguilar, Reynaldo; Hernández‐Guerrero, Juan Carlos; Cravioto, Alejandro

doi:10.1111/j.1399-302x.2009.00541.x

Cited by 31 publications

(24 citation statements)

References 21 publications

(53 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A large number of PCR systems used for the detection of H. pylori DNA in gastric biopsy samples and oral samples have been reported (Silva et al, 2009;Morales-Espinosa et al, 2009). With the exception of H. pylori, very few bacteria are observed in gastric lesions because of the highly acidic pH (Hirai et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Detection of Helicobacter pylori DNA in inflamed dental pulp specimens from Japanese children and adolescents

Ogaya

Nomura

Watanabe

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

The oral cavity has been implicated as a source of Helicobacter pylori infection in childhood. Various PCR methods have been used to detect H. pylori DNA in oral specimens with various detection rates reported. Such disparity in detection rates complicates the estimation of the true infection rate of H. pylori in the oral cavity. In the present study, we constructed a novel PCR system for H. pylori detection and used it to analyse oral specimens. Firstly, the nucleotide alignments of genes commonly used for H. pylori detection were compared using the complete genome information for 48 strains registered in the GenBank database. Candidate primer sets with an estimated amplification size of approximately 300-400 bp were selected, and the specificity and sensitivity of the detection system using each primer set were evaluated. Five sets of primers targeting ureA were considered appropriate, of which a single primer set was chosen for inclusion in the PCR system. The sensitivity of the system was considered appropriate and its detection limit established as one to ten cells per reaction. The novel PCR system was used to examine H. pylori distribution in oral specimens (40 inflamed pulp tissues, 40 saliva samples) collected from Japanese children, adolescents and young adults. PCR analysis revealed that the detection rate of H. pylori in inflamed pulp was 15 %, whereas no positive reaction was found in any of the saliva specimens. Taken together, our novel PCR system was found to be reliable for detecting H. pylori. The results obtained showed that H. pylori was detected in inflamed pulp but not saliva specimens, indicating that an infected root canal may be a reservoir for H. pylori. INTRODUCTIONHelicobacter pylori is a Gram-negative microaerophilic bacterium responsible for chronic gastritis, peptic ulcers and gastric cancer (Fennerty, 1994). Details regarding transmission and infection source are controversial, and it has been suggested that most infections are acquired in childhood, mainly via the oral cavity (Prasanthi et al., 2011). H. pylori has been isolated from, and its DNA detected in, gastric biopsy and faecal samples (Momtaz et al., 2012). In addition, several studies have detected H. pylori bacterial DNA in saliva and dental plaque specimens (Song et al., 1999;Kignel et al., 2005).Clinical detection of H. pylori infection in gastric tissue is achieved using the urea breath test, a urease test, histology, culture and serology results (Goodwin et al., 1997); whilst molecular biological methods using PCR assays are widely used for the detection of H. pylori in the oral cavity with high sensitivity (Westblom & Bhatt, 1999). Published PCR methods used for the detection of H. pylori have targeted genes including 16S rRNA, vacA, cagA, glmM (ureC) and ureA genes (Mapstone et al., 1993;Miyabayashi et al., 2000;Wang et al., 2002;Park et al., 2003;Smith et al., 2004). Reports of PCR detection of H. pylori have described good sensitivity and/or specificity of tested primer sets, although detection rates for the ora...

show abstract

Section: Discussionmentioning

confidence: 99%

Detection of Helicobacter pylori DNA in inflamed dental pulp specimens from Japanese children and adolescents

Ogaya

Nomura

Watanabe

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…The present study suggests and could expands on that theory by demonstrating that not only are oral-sexual behaviours important, but m mmon habits, such as smoking or drinking alcohol, may also contribute to the HPV infection to the oral region. It could be interesting to evaluate whether the oral cavity could be not only the reservoir for HPV, but for other pathogens [47]. The results of the present investigation showed a high frequency of HPV (67%) infection in the oral and genital mucosas, suggesting that patient's habits could contribute to the infection; and they suggest that the mode of viral transmission.…”

Section: Pv-dn Hmentioning

confidence: 69%

Low and High Risk Human Papillomavirus in the Oral Mucosa of Mexican Women with Genital Papillomavirus

Fernández¹,

Rosete²,

Pedraza³

et al. 2013

OJMM

View full text Add to dashboard Cite

Background: Human papillomaviruses (HPV) are implicated in cervical cancer and, recently in oral cancer. In Mexico, there are few studies on oral cancer, therefore the interest in identifying the HPV frequency of low and high risk in samples of the oral and cervical cavities, and determining some risk factors. Objective: To determine the frequency of high and low risk HPV infection in the oral cavity of women with cervical HPV, and to correlate the infection site with risk factors. Materials and Methods: Eighteen female patients between 24 and 53 years, with antecedents of genital HPV infection were included. Both samples of oral cavity and cervix were obtained. DNA extraction from the epithelial cells was performed using the Qiagen kit. PCR was done and the amplicon was observed in 2% agarose gels stained with ethidium bromide. A correlation of HPV infection and risk factors was done. Results: HPV-DNA was detected in the 67% of both samples. The frequency of oral and cervix low risk HPV-DNA was 50%, while high risk HPV-DNA in oral cavity was detected in 17%, and 39% in the cervix. The study of the risk factors involved in HPV infection showed that the participants had the habits of smoking 39%; alcohol drinking 28%; and 78% oral sex. Conclusion: The results showed a high frequency of HPV (67%) infection in the oral and genital mucosas, suggesting that patient's habits could contribute to the infection; the presence of HPV in the oral mucosa may act as reservoir for new HPV infections.

show abstract

“…Moreover, on the basis of PCR results, some authors have suggested that saliva and dental plaque may serve as a temporary reservoir for H. pylori in individuals with gastric disease (Silva et al, 2009). Even an antibacterial treatment therapy was recommended by some researchers on the basis of positive PCR results of H. pylori in the oral cavity of patients with gastric symptoms, since the authors have suggested an association between the prevalence of H. pylori in the oral cavity and gastroesophageal disease (Morales-Espinosa et al, 2009). …”

Section: ; De Souza Gonçalves Et Al 2009)mentioning

confidence: 99%

Is Helicobacter pylori resident or transient in the human oral cavity?

Al‐Ahmad

Kürschner

Weckesser

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

Helicobacter pylori colonizes the stomachs of at least half of the world's human population. The role of the oral cavity in this colonization is not clear and there are, to date, no comprehensive data that clearly demonstrate the isolation of this bacterium from the oral cavity. The aim of this study was to evaluate the prevalence of H. pylori in the oral cavity of 15 patients who tested positive for H. pylori. A comprehensive dental examination of all patients was conducted. Samples were taken from supragingival and subgingival plaque, saliva, periapical exudates and tongue swabs. All samples were taken before the application of antibiotics. A total of 163 oral samples were investigated by PCR using two different H. pylori-specific primer pairs. A PCR inhibition control using a modified plasmid was always included for the most specific primer pair. In addition, a culture technique was used to confirm PCR results. Despite a PCR detection limit of 10 2 bacteria ml "1, out of 14 patients, H. pylori could not be detected in any of the samples taken. In one patient, H. pylori-positive PCR signals were obtained in two samples using only one primer pair. H. pylori could not be cultivated from these two PCR-positive samples; therefore, no correlation to oral colonization status could be established. This study challenges the misleading preconception that H. pylori resides in the human oral cavity and suggests that this bacterium should be considered transient and independent of the oral status. To date, positive PCR results for H. pylori in the oral cavity have been overestimated and not critically interpreted in literature.

show abstract

Helicobacter pylori in the oral cavity is associated with gastroesophageal disease

Abstract: H. pylori detection in the oral cavity is associated to gastroesophageal disease. In addition, it is suggested that all patients presenting gastric symptoms and H. pylori detection in the oral cavity would begin bacterial treatment immediately.

Cited by 31 publications

References 21 publications

Detection of Helicobacter pylori DNA in inflamed dental pulp specimens from Japanese children and adolescents

Detection of Helicobacter pylori DNA in inflamed dental pulp specimens from Japanese children and adolescents

Low and High Risk Human Papillomavirus in the Oral Mucosa of Mexican Women with Genital Papillomavirus

Is Helicobacter pylori resident or transient in the human oral cavity?

Contact Info

Product

Resources

About