<i>Helicobacter pylori</i> induces lymphocyte activation in peripheral blood cultures

Karttunen, Riitta; Andersson, Gun; Poikonen, Kari; Kosunen, Timo U.; Karttunen, Tuomo J.; Juutinen, K; Niemelä, Seppo

doi:10.1111/j.1365-2249.1990.tb05476.x

Cited by 85 publications

(18 citation statements)

References 13 publications

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“…However, since circulating T cells from both infected and uninfected individuals in countries with lower prevalence of H. pylori also respond similarly to H. pylori antigens after both short and longer stimulation [7], [48], [49], we cannot rule out that the responses detected may also partially be a result of cross-reactivity with other bacteria expressing similar antigens. Due to the small amount of blood available, we could only test MP from one H. pylori strain.…”

Section: Discussionmentioning

confidence: 99%

Th1 and Th17 Responses to Helicobacter pylori in Bangladeshi Infants, Children and Adults

et al. 2014

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Both Th1 and Th17 cells are important components of the immune response to Helicobacter pylori (Hp) in adults, but less is known about T cell responses to Hp during early childhood, when the infection is often acquired. We investigated Th1 and Th17 type responses to Hp in adults, children and infants in Bangladesh, where Hp is highly endemic. IL-17 and IFN-γ mRNA levels in gastric biopsies from Hp-infected Bangladeshi adults were analyzed and compared to levels in infected and uninfected Swedish controls. Since biopsies could not be collected from infants and children, cytokine responses in Bangladeshi infants (6–12 months), children (3–5 years) and adults (>19 years) were instead compared by stimulating peripheral blood mononuclear cells (PBMCs) with a Hp membrane preparation (MP) and analyzing culture supernatants by ELISA and cytometric bead array. We found significantly higher expression of IL-17 and IFN-γ mRNA in gastric mucosa of Hp-infected Bangladeshi and Swedish adults compared to uninfected Swedish controls. PBMCs from all age groups produced IL-17 and IFN-γ after MP stimulation, but little Th2 cytokines. IL-17 and IFN-γ were primarily produced by CD4+ T cells, since CD4+ T cell depleted PBMCs produced reduced amounts of these cytokines. Infant cells produced significantly more IL-17, but similar levels of IFN-γ, compared to adult cells after MP stimulation. In contrast, polyclonal stimulation induced lower levels IL-17 and IFN-γ in infant compared to adult PBMCs and CD4+ T cells. The strong IL-17 production in infants after MP stimulation was paralleled by significantly higher production of the IL-17 promoting cytokine IL-1β from infant compared to adult PBMCs and monocytes. In conclusion, these results show that T cells can produce high levels of IL-17 and IFN-γ in response to Hp from an early age and indicate a potential role for IL-1β in promoting Th17 responses to Hp during infancy.

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Section: Discussionmentioning

confidence: 99%

Th1 and Th17 Responses to Helicobacter pylori in Bangladeshi Infants, Children and Adults

et al. 2014

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“…6–9 For example, adoptive transfer of CD4 + CD45RB hi effector T cells from naive donors into immunodeficient recipients causes severe gastritis in H. pylori -infected recipients, whereas co-transfer of CD4 + CD45RB lo regulatory T cells (T reg ) protects against gastritis. 10 Several studies have suggested that CD4 + /CD25 + /Foxp3 + T reg control gastric inflammation and contribute to the persistence of H. pylori infections.…”

Section: Introductionmentioning

confidence: 99%

A2A adenosine receptor (AR) activation inhibits pro-inflammatory cytokine production by human CD4+ helper T cells and regulates Helicobacter-induced gastritis and bacterial persistence

et al. 2009

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“…Several studies suggest that T helper (Th) type 1 (Th1) cells are selectively increased in response to gastric infection [7–10]. Other data have demonstrated that different subpopulations of CD4 + T lymphocytes play distinct roles in mediating and regulating H. pylori –induced gastritis.…”

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CD73 Is Expressed by Human Regulatory T Helper Cells and Suppresses Proinflammatory Cytokine Production andHelicobacter felis–Induced Gastritis in Mice

et al. 2009

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Background Regulatory T cells (known as “Treg”) express apyrases (CD39) and ecto-5'-nucleotidase (CD73) and contribute to their inhibitory function by generating adenosine. We investigated the expression of CD39 and CD73 on human T helper (Th) cells and the role of CD73 in regulating Helicobacter felis–induced gastritis and colonization. Methods Human CD4+ Th cells, gastric T cells, or Treg subsets were stimulated and assayed for the expression of CD39 and CD73 by means of reverse-transcriptase polymerase chain reaction and flow cytometry. The effect of CD73 on proliferation and cytokine production was assessed, and the presence of gastritis, proinflammatory cytokine expression, or colonization of H. felis was evaluated in CD73-deficient (CD73–/–) mice or recipient mice given control or CD73–/– Treg. Results CD4+ T cells expressed CD39 and CD73, particularly in CD25+Foxp3+ Treg from peripheral blood or gastric mucosa. Activation significantly increased CD73 expression on all Th cells. Inhibition of CD73 enhanced production of interferon–γ. Gastritis in H. felis–infected CD73–/– mice was significantly worse than that in wild-type mice and was accompanied by increased levels of proinflammatory cytokines and reduced bacterial colonization, whereas Treg from CD73–/– mice did not inhibit gastritis. Conclusion CD39 and CD73 expressed by Th cells contribute to local accumulation of adenosine and attenuation of gastritis, which may favor persistent infection.

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Helicobacter pylori induces lymphocyte activation in peripheral blood cultures

Cited by 85 publications

References 13 publications

Th1 and Th17 Responses to Helicobacter pylori in Bangladeshi Infants, Children and Adults

Th1 and Th17 Responses to Helicobacter pylori in Bangladeshi Infants, Children and Adults

A2A adenosine receptor (AR) activation inhibits pro-inflammatory cytokine production by human CD4+ helper T cells and regulates Helicobacter-induced gastritis and bacterial persistence

CD73 Is Expressed by Human Regulatory T Helper Cells and Suppresses Proinflammatory Cytokine Production andHelicobacter felis–Induced Gastritis in Mice

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