HLA‐B*15 subtypes in Burmese population by sequence‐based typing

Abstract

Human leukocyte antigen (HLA)-B*15 encompasses an increasing number of subtypes of more than 150. Frequency studies and a strong genetic association between HLA subtypes and susceptibility to drug hypersensitivity have been reported in different ethnic populations. To identify HLA-B*15 subtypes in Burmese using sequence-based typing (SBT) method, we selected 65 HLA-B*15-positive samples from 170 unrelated healthy Burmese who were genotyped HLA-B* by polymerase chain reaction with the sequence-specific primer method. The frequency of HLA-B*15 in Burmese was found to be 38.2%. By the SBT method, results showed 10 alleles of HLA-B*15 subtypes. Four common alleles, B*1502 (45.2%), B*1532 (16.4%), B*1525 (12.3%), and B*1501 (8.2%), were found in 82.1% of HLA-B*15-positive Burmese. Whereas the B*1501 was the highest in the Caucasians, Koreans, and Japanese, the highest frequency of HLA-B*15 alleles in Burmese was B*1502 (45.2%) that is similar to the frequency found in northeastern Thais and Vietnamese. This study is the first report of HLA-B*15 subtypes in Burmese. These results will provide the basic data in the further study in transplantations, genetic association with diseases, and drug hypersensitivity.

Large-scale mitochondrial DNA analysis in Southeast Asia reveals evolutionary effects of cultural isolation in the multi-ethnic population of Myanmar

“…The magnitude of the observed migration rates could reflect the widely shared population history of Myanmar, Thailand, Laos, Vietnam and South China, all descending from Sino-Tibetan tribes [11,14,15]. However, the majority of the present genetic exchange, particularly with the Thai population, seems to originate from the Karen minority: The Thai samples included in this study come from the Chiang Mai region [29], where traditionally many Karen live, nowadays a considerable proportion of them as refugees [46]. Migration analyses, especially the dominating outwards migration, could therefore also indicate an active marginalization and displacement of ethnic minorities, especially of the Karen people with ~140,000 Karen refugees alone in Thailand.…”

Pharmacogenetic screening of carbamazepine-induced severe cutaneous allergic reactions

“…A high prevalence of 5% is also seen in Asians residing in North America. 32 The HLA-B ⁄ 1502 is one of the most common HLA-B alleles among Thai, 33 Burmese, 34 Vietnamese 35 and Western Javanese 36 but is rare in Caucasians. In a prior study of European populations, the allele frequency was only 1% to 2%.…”

FCGR3B gene frequencies among ethnic Thai blood donors from a regional hospital in Eastern Thailand

“…They were also asked for their informed consent, in accordance with the Human Research Ethics committee in Huachiew Chalermprakiet University. The buffy coat was lysed by red blood cell lysis buffer, and then the genomic DNA was extracted by using guanidine method . The genotyping system was validated by using DNA from FCGR3B*01+, *02−, *03− , FCGR3B*01−, *02+, *03− and FCGR3B*01+, *02+, *03− individuals, which had previously been determined by DNA sequencing method.…”