<i>HOX</i> genes are expressed in bovine and mouse oocytes and early embryos

Paul, Delphine; Bridoux, Laure; Rezsöhazy, René; Donnay, Isabelle

doi:10.1002/mrd.21321

Cited by 20 publications

(18 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, many other homeobox genes also cluster in this set on the basis of their expression, including two Hox genes ( HOXD1, HOXC13 ) indicating they are worthy of further study in this regard (Additional file 5: Table S6). Hoxd1 expression has been previously reported in preimplantation mouse and cow embryos [28–30] but not to our knowledge Hoxc13 ; however, one of the two hoxc13 duplicates in zebrafish is expressed in early cleavage stages [31]. …”

Section: Resultsmentioning

confidence: 99%

Diversity of human and mouse homeobox gene expression in development and adult tissues

Dunwell

Holland

2016

BMC Dev Biol

View full text Add to dashboard Cite

BackgroundHomeobox genes encode a diverse set of transcription factors implicated in a vast range of biological processes including, but not limited to, embryonic cell fate specification and patterning. Although numerous studies report expression of particular sets of homeobox genes, a systematic analysis of the tissue specificity of homeobox genes is lacking.ResultsHere we analyse publicly-available transcriptome data from human and mouse developmental stages, and adult human tissues, to identify groups of homeobox genes with similar expression patterns. We calculate expression profiles for 242 human and 278 mouse homeobox loci across a combination of 59 human and 12 mouse adult tissues, early and late developmental stages. This revealed 20 human homeobox genes with widespread expression, primarily from the TALE, CERS and ZF classes. Most homeobox genes, however, have greater tissue-specificity, allowing us to compile homeobox gene expression lists for neural tissues, immune tissues, reproductive and developmental samples, and for numerous organ systems. In mouse development, we propose four distinct phases of homeobox gene expression: oocyte to zygote; 2-cell; 4-cell to blastocyst; early to mid post-implantation. The final phase change is marked by expression of ANTP class genes. We also use these data to compare expression specificity between evolutionarily-based gene classes, revealing that ANTP, PRD, LIM and POU homeobox gene classes have highest tissue specificity while HNF, TALE, CUT and CERS are most widely expressed.ConclusionsThe homeobox genes comprise a large superclass and their expression patterns are correspondingly diverse, although in a broad sense related to an evolutionarily-based classification. The ubiquitous expression of some genes suggests roles in general cellular processes; in contrast, most human homeobox genes have greater tissue specificity and we compile useful homeobox datasets for particular tissues, organs and developmental stages. The identification of a set of eutherian-specific homeobox genes peaking from human 8-cell to morula stages suggests co-option of new genes to new developmental roles in evolution.Electronic supplementary materialThe online version of this article (doi:10.1186/s12861-016-0140-y) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

Diversity of human and mouse homeobox gene expression in development and adult tissues

Dunwell

Holland

2016

BMC Dev Biol

View full text Add to dashboard Cite

show abstract

“…Bovine embryos were produced, as previously described [24]. In brief, bovine ovaries were collected at a local slaughterhouse.…”

Section: Methodsmentioning

confidence: 99%

“…Female mice (8- to 9-week-old) were superovulated, as previously described [24]. Immature oocytes were harvested 47 h post-eCG whereas mature oocytes were collected 14 h post-hCG injection.…”

Section: Methodsmentioning

confidence: 99%

“…Superovulated mice were mated overnight (O/N) and euthanized 21 h post-hCG to collect presumptive zygotes from the oviductal ampulla. Zygotes were then denuded from their cumulus cells and cultured, as previously described [24]. Two-cell embryos, pre-compaction morulae, compact morulae, early blastocysts and blastocysts were collected 48 h (E1.5), 72 h (E2.5), 80 h (E2.8), 92 h (E3.3) and 100 h (E3.6) post-hCG injection, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…In particular, we have previously shown that HOXB9 transcripts are present in oocytes and early embryos in the mouse and bovine [24]. In the bovine, the relative expression of HOXB9 increases between the immature oocyte and the zygote stage, further increases at the 5- to 8-cell stage and peaks at the morula stage before decreasing at the blastocyst stage.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dynamic Pattern of HOXB9 Protein Localization during Oocyte Maturation and Early Embryonic Development in Mammals

et al. 2016

Self Cite

View full text Add to dashboard Cite

BackgroundWe previously showed that the homeodomain transcription factor HOXB9 is expressed in mammalian oocytes and early embryos. However, a systematic and exhaustive study of the localization of the HOXB9 protein, and HOX proteins in general, during mammalian early embryonic development has so far never been performed.ResultsThe distribution of HOXB9 proteins in oocytes and the early embryo was characterized by immunofluorescence from the immature oocyte stage to the peri-gastrulation period in both the mouse and the bovine. HOXB9 was detected at all studied stages with a dynamic expression pattern. Its distribution was well conserved between the two species until the blastocyst stage and was mainly nuclear. From that stage on, trophoblastic cells always showed a strong nuclear staining, while the inner cell mass and the derived cell lines showed important dynamic variations both in staining intensity and in intra-cellular localization. Indeed, HOXB9 appeared to be progressively downregulated in epiblast cells and only reappeared after gastrulation had well progressed. The protein was also detected in the primitive endoderm and its derivatives with a distinctive presence in apical vacuoles of mouse visceral endoderm cells.ConclusionsTogether, these results could suggest the existence of unsuspected functions for HOXB9 during early embryonic development in mammals.

show abstract

Characterization of TALE genes expression during the first lineage segregation in mammalian embryos

Sonnet

Rezsöhazy

Donnay

2012

Developmental Dynamics

Self Cite

View full text Add to dashboard Cite

Background: Three amino acid loop extension (TALE) homeodomain-containing transcription factors are generally recognized for their role in organogenesis and differentiation during embryogenesis. However, very little is known about the expression and function of Meis, Pbx, and Prep genes during early development. Results: In order to determine whether TALE proteins could contribute to the early cell fate decisions in mammalian development, this study aimed to characterize in a systematic manner the pattern of expression of all Meis, Pbx, and Prep genes from the precompaction to blastocyst stage corresponding to the first step of cell differentiation in mammals. To reveal to what extent TALE genes expression at these early stages is a conserved feature among mammals, this study was performed in parallel in the bovine and mouse models. We demonstrated the transcription and translation of TALE genes, before gastrulation in the two species. At least one member of Meis, Pbx, and Prep subfamilies was found expressed at the RNA and protein levels but different patterns of expression were observed between genes and between species, suggesting specific gene regulations. Conclusions: Taken together, these results suggest a previously unexpected involvement of these factors during the early development in mammals. Developmental Dynamics 241:1827-1839, 2012. V C 2012 Wiley Periodicals, Inc.Key words: Meis; Pbx; Prep; TALE; homeodomain proteins; preimplantation development; bovine; mouse Key findings:By a systematic approach, we demonstrated the transcription and translation of TALE genes before gastrulation during the first steps of cell differentiation in bovine and in mouse embryos. TALE genes are expressed during the first cell lineage specification in mammals. TALE proteins are present before gastrulation in mammalian morulae and blastocysts. TALE genes expression is differently regulated in mouse and bovine early embryos. TALE mRNA and protein levels are well correlated in early mammalian embryos. TALE proteins are found both in the inner cell mass and trophectoderm cells of mammalian blastocysts.

show abstract

HOX genes are expressed in bovine and mouse oocytes and early embryos

Cited by 20 publications

References 41 publications

Diversity of human and mouse homeobox gene expression in development and adult tissues

Diversity of human and mouse homeobox gene expression in development and adult tissues

Dynamic Pattern of HOXB9 Protein Localization during Oocyte Maturation and Early Embryonic Development in Mammals

Characterization of TALE genes expression during the first lineage segregation in mammalian embryos

Contact Info

Product

Resources

About