<i>Hsp60C</i> is required in follicle as well as germline cells during oogenesis in <i>Drosophila melanogaster</i>

Sarkar, Surajit; Lakhotia, S. C.

doi:10.1002/dvdy.21524

Cited by 16 publications

(16 citation statements)

References 49 publications

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“…The close proximity of DIAP1 with F-actin, which plays a critical role in photoreceptor morphogenesis (Benlali et al 2000;Tepass and Harris 2007), suggests that, as reported in other cell types Montell 2006;Oshima et al 2006), a nonapoptotic function of DIAP1 in eye discs may relate to actin dynamics and thus photoreceptor morphogenesis. Other studies in our laboratory (Sarkar and Lakhotia 2008) have revealed an essential role of Hsp60C in organizing F-actin and other cytoskeletal structures in follicle and germ cells in developing egg chambers. It is, therefore, possible that in differentiating photoreceptor cells, the Hsp60D, together with DIAP1 may regulate the cytoskeletal remodeling of the specific architecture of these highly specialized cells.…”

Section: Discussionmentioning

confidence: 71%

Hsp60D is essential for caspase-mediated induced apoptosis in Drosophila melanogaster

Arya

Lakhotia

2008

Cell Stress and Chaperones

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Apart from their roles as chaperones, heat shock proteins are involved in other vital activities including apoptosis with mammalian Hsp60 being ascribed proapoptotic as well as antiapoptotic roles. Using conditional RNAi or overexpression of Hsp60D, a member of the Hsp60 family in Drosophila melanogaster, we show that the downregulation of this protein blocks caspase-dependent induced apoptosis. GMR-Gal4-driven RNAi for Hsp60D in developing eyes dominantly suppressed cell death caused by expression of Reaper, Hid, or Grim (RHG), the key activators of canonical cell death pathway. Likewise, Hsp60D-RNAi rescued cell death induced by GMR-Gal4-directed expression of full-length and activated DRONC. Overexpression of Hsp60D enhanced cell death induced either by directed expression of RHG or DRONC. However, the downregulation of Hsp60D failed to suppress apoptosis caused by unguarded caspases in DIAP1-RNAi flies. Furthermore, in DIAP1-RNAi background, Hsp60D-RNAi also failed to inhibit apoptosis induced by RHG expression. The Hsp60 and DIAP1 show diffuse and distinct granular overlapping distributions in the photoreceptor cells with the bulk of both proteins being outside the mitochondria. Depletion of either of these proteins disrupts the granular distribution of the other. We suggest that in the absence of Hsp60D, DIAP1 is unable to dissociate from effecter and executioner caspases, which thus remain inactive.

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Section: Discussionmentioning

confidence: 71%

Hsp60D is essential for caspase-mediated induced apoptosis in Drosophila melanogaster

Arya

Lakhotia

2008

Cell Stress and Chaperones

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“…temperature), but is also essential during many developmental processes, including oogenesis. It is thought that Hsps are important for both developmental buffering and differentiation [72,186](further references in Additional file 1). The functional contexts in which Hsps operate are incredibly varied [186].…”

Section: Discussionmentioning

confidence: 99%

“…It is thought that Hsps are important for both developmental buffering and differentiation [72,186](further references in Additional file 1). The functional contexts in which Hsps operate are incredibly varied [186]. In D. melanogaster , for example, Hsp60C is essential in organising and maintaining cytoskeletal and cell adhesion components and thus for establishing AP and DV oocyte polarity [186], whilst Hsp70 affects border cell migration through its effects on the actin cytoskeleton [187].…”

Section: Discussionmentioning

confidence: 99%

“…The functional contexts in which Hsps operate are incredibly varied [186]. In D. melanogaster , for example, Hsp60C is essential in organising and maintaining cytoskeletal and cell adhesion components and thus for establishing AP and DV oocyte polarity [186], whilst Hsp70 affects border cell migration through its effects on the actin cytoskeleton [187]. A large number of genes encoding Hsps and related proteins have been described in a functional context during D. melanogaster oogenesis (references in Additional file 1) and orthologs of all of these were transcribed during P. aegeria ovarioles, often very abundantly (e.g.…”

Section: Discussionmentioning

confidence: 99%

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Unscrambling butterfly oogenesis

Carter

Baker²,

Pink

et al. 2013

BMC Genomics

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BackgroundButterflies are popular model organisms to study physiological mechanisms underlying variability in oogenesis and egg provisioning in response to environmental conditions. Nothing is known, however, about; the developmental mechanisms governing butterfly oogenesis, how polarity in the oocyte is established, or which particular maternal effect genes regulate early embryogenesis. To gain insights into these developmental mechanisms and to identify the conserved and divergent aspects of butterfly oogenesis, we analysed a de novo ovarian transcriptome of the Speckled Wood butterfly Pararge aegeria (L.), and compared the results with known model organisms such as Drosophila melanogaster and Bombyx mori.ResultsA total of 17306 contigs were annotated, with 30% possibly novel or highly divergent sequences observed. Pararge aegeria females expressed 74.5% of the genes that are known to be essential for D. melanogaster oogenesis. We discuss the genes involved in all aspects of oogenesis, including vitellogenesis and choriogenesis, plus those implicated in hormonal control of oogenesis and transgenerational hormonal effects in great detail. Compared to other insects, a number of significant differences were observed in; the genes involved in stem cell maintenance and differentiation in the germarium, establishment of oocyte polarity, and in several aspects of maternal regulation of zygotic development.ConclusionsThis study provides valuable resources to investigate a number of divergent aspects of butterfly oogenesis requiring further research. In order to fully unscramble butterfly oogenesis, we also now also have the resources to investigate expression patterns of oogenesis genes under a range of environmental conditions, and to establish their function.

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“…The Zdisc in light bands also showed presence of Hsp60. In addition, a close interaction was also evident between Hsp60 and cytoskeletal components during Drosophila oogenesis being essential for microtubule remodeling, cell-cell adhesion and determination of oocyte polarity (Sarkar and Lakhotia, 2008). Abnormal arrangement of F-actin-based cytoskeleton in Drosophila Hsp60C mutant allele suggests a pivotal role of this gene in maintenance of cellular integrity (Fig.…”

Section: Hsp60 and Cytoskeleton Proteinsmentioning

confidence: 92%

Heat shock proteins: Molecules with assorted functions

et al. 2011

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Heat shock proteins (Hsps) or molecular chaperones, are highly conserved protein families present in all studied organisms. Following cellular stress, the intracellular concentration of Hsps generally increases several folds. Hsps undergo ATP-driven conformational changes to stabilize unfolded proteins or unfold them for translocation across membranes or mark them for degradation. They are broadly classified in several families according to their molecular weights and functional properties. Extensive studies during the past few decades suggest that Hsps play a vital role in both normal cellular homeostasis and stress response. Hsps have been reported to interact with numerous substrates and are involved in many biological functions such as cellular communication, immune response, protein transport, apoptosis, cell cycle regulation, gametogenesis and aging. The present review attempts to provide a brief overview of various Hsps and summarizes their involvement in diverse biological activities.

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Hsp60C is required in follicle as well as germline cells during oogenesis in Drosophila melanogaster

Cited by 16 publications

References 49 publications

Hsp60D is essential for caspase-mediated induced apoptosis in Drosophila melanogaster

Hsp60D is essential for caspase-mediated induced apoptosis in Drosophila melanogaster

Unscrambling butterfly oogenesis

Heat shock proteins: Molecules with assorted functions

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