<i>Ift88</i> regulates enamel formation via involving Shh signaling

Kudo, Takehisa; Kawasaki, Maiko; Kawasaki, Kazumasa; Meguro, Fumiya; Nihara, Jun; Honda, Izumi; Kitamura, Madoka; Fujita, Akira; Osawa, Kazuaki; Ichikawa, Kaya; Nagai, Takahiro; Ishida, Yasushi; Sharpe, Paul T.; Maeda, Takeyasu; Saito, Isao; Ohazama, Atsushi

doi:10.1111/odi.14162

Cited by 5 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SA00852), and all procedures were performed under protocols approved by the Institutional Animal Care and Use Committee and followed the Animal Research: Reporting of In Vivo Experiments (ARRIVE 2.0) guidelines. Atg7 f/f mice (Komatsu et al 2005) were bred with KRT14-Cre mice (Kudo et al 2022) to generate Atg7 f/f ;KRT14-Cre mice. To induce type 1 diabetes, streptozotocin (STZ) (Sigma-Aldrich) was injected intraperitoneally in these mice.…”

Section: Micementioning

confidence: 99%

Loss of Autophagy Disrupts Stemness of Ameloblast-Lineage Cells in Aging

Ida-Yonemochi,

Otsu,

Irié

et al. 2023

J Dent Res

Self Cite

View full text Add to dashboard Cite

Autophagy is one of the intracellular degradation pathways and maintains cellular homeostasis, regulating the stress response, cell proliferation, and signal transduction. To elucidate the role of autophagy in the maintenance of dental epithelial stem cells and the subsequent enamel formation, we analyzed autophagy-deficient mice in epithelial cells (Atg7f/f;KRT14-Cre mice), focusing on the influence of aging and stress environments. We also performed in vitro cell and organ culture experiments with an autophagy inhibitor. In young Atg7f/f;KRT14-Cre mice, morphological change was not obvious in maxillary incisors, except for the remarkable cell death in the stratum intermedium of the transitional stage. However, under stress conditions of hyperglycemia, the incisor color changed to white in diabetes Atg7f/f;KRT14-Cre mice. Regarding dental epithelial stem cells, the shape of the apical bud region of the incisor became irregular with age, and odontoma was formed in aged Atg7f/f;KRT14-Cre mice. In addition, the shape of apical bud culture cells of Atg7f/f;KRT14-Cre mice became irregular and enlarged atypically, with epigenetic changes during culture, suggesting that autophagy deficiency may induce tumorigenesis in dental epithelial cells. The epigenetic change and upregulation of p21 expression were induced by autophagy inhibition in vivo and in vitro. These findings suggest that autophagy is important for the regulation of stem cell maintenance, proliferation, and differentiation of ameloblast-lineage cells, and an autophagy disorder may induce tumorigenesis in odontogenic epithelial cells.

show abstract

Section: Micementioning

confidence: 99%

Loss of Autophagy Disrupts Stemness of Ameloblast-Lineage Cells in Aging

Ida-Yonemochi,

Otsu,

Irié

et al. 2023

J Dent Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…The role of primary cilia in enamel production has also been evaluated using a conditional deletion of Ift88 in epithelial cells ( Keratin14 ; Ift88 fl/fl ) [ 30 ]. K14 ; Ift88 fl/fl mice had chalky incisors and roughened enamel surfaces on all molars due to abrasions that became more severe with time.…”

Section: Primary Cilia In Craniofacial Developmentmentioning

confidence: 99%

Primary Cilia: The New Face of Craniofacial Research

Moore

2022

Biomolecules

View full text Add to dashboard Cite

The primary cilium is a solitary, sensory organelle that extends from the surface of nearly every vertebrate cell, including craniofacial cells. This organelle converts chemical and physical external stimuli into intracellular signaling cascades and mediates several well-known signaling pathways simultaneously. Thus, the primary cilium is considered a cellular signaling nexus and amplifier. Primary cilia dysfunction directly results in a collection of diseases and syndromes that typically affect multiple organ systems, including the face and teeth. Despite this direct connection, primary cilia are largely unexplored in craniofacial research. In this review, I briefly summarize craniofacial abnormalities tied to the primary cilium and examine the existing information on primary cilia in craniofacial development and repair. I close with a discussion on preliminary studies that motivate future areas of exploration that are further supported by studies performed in long bone and kidney cells.

show abstract

Primary Cilia Regulate the Homeostasis and Regeneration of the Stem Cell Niche in the Tooth

Zhang,

Cao,

Wang

et al. 2024

Journal Cellular Physiology

View full text Add to dashboard Cite

Primary cilia, functioning as crucial hubs for signal sensing and transduction, are integral to the development and maintenance of homeostasis across various organs. However, their roles in tooth homeostasis and repair remain inadequately understood. In this study, we reveal an indispensable role for primary cilia in regulating the homeostasis and regeneration of teeth, primarily through the regulation of cell proliferation. Using cilium‐deficient mice, we demonstrate that disruption of ciliary homeostasis leads to abnormal tooth morphology, stunted growth and notably impaired tooth repair. RNA sequencing reveals a dysregulation in genes associated with various biological processes such as cell proliferation, differentiation, and cycle regulation. Furthermore, we show that cilium‐deficient mice display reduced cell proliferation. Our findings highlight a critical function for primary cilia in the regulation of tooth homeostasis and regeneration and have important implications for the development of tooth regeneration therapies.

show abstract

Ift88 regulates enamel formation via involving Shh signaling

Cited by 5 publications

References 40 publications

Loss of Autophagy Disrupts Stemness of Ameloblast-Lineage Cells in Aging

Loss of Autophagy Disrupts Stemness of Ameloblast-Lineage Cells in Aging

Primary Cilia: The New Face of Craniofacial Research

Primary Cilia Regulate the Homeostasis and Regeneration of the Stem Cell Niche in the Tooth

Contact Info

Product

Resources

About