<i>In‐field</i> detection and quantification of extracellular DNA

Ceccherini, Maria Teresa; Ascher, Judith; Pietramellara, Giacomo

doi:10.1002/jpln.200900081

Cited by 9 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plants may also employ PRR(s) to perceive extracellular DNA (eDNA), as eDNA induces PTI-like defenses and pathogen resistance (Wen et al, 2009;Heil, 2014, 2015;Mazzoleni et al, 2015;Gallucci and Maffei, 2017). Of note, substantial amounts of DNA are secreted from plant roots, and influence both plants and microbes in the soil (Ceccherini et al, 2009;Driouich et al, 2013). eDNA serves as a nutrient source (Paungfoo-Lonhienne et al, 2010), but also causes plant growth inhibition in a conspecific manner (Mazzoleni et al, 2015).…”

Section: Recognition Of Common Patterns Across Kingdomsmentioning

confidence: 99%

Pattern recognition receptors and signaling in plant–microbe interactions

2018

View full text Add to dashboard Cite

SUMMARYPlants solely rely on innate immunity of each individual cell to deal with a diversity of microbes in the environment. Extracellular recognition of microbe-and host damage-associated molecular patterns leads to the first layer of inducible defenses, termed pattern-triggered immunity (PTI). In plants, pattern recognition receptors (PRRs) described to date are all membrane-associated receptor-like kinases or receptor-like proteins, reflecting the prevalence of apoplastic colonization of plant-infecting microbes. An increasing inventory of elicitor-active patterns and PRRs indicates that a large number of them are limited to a certain range of plant groups/species, pointing to dynamic and convergent evolution of pattern recognition specificities. In addition to common molecular principles of PRR signaling, recent studies have revealed substantial diversification between PRRs in their functions and regulatory mechanisms. This serves to confer robustness and plasticity to the whole PTI system in natural infections, wherein different PRRs are simultaneously engaged and faced with microbial assaults. We review the functional significance and molecular basis of PRRmediated pathogen recognition and disease resistance, and also an emerging role for PRRs in homeostatic association with beneficial or commensal microbes.

show abstract

Section: Recognition Of Common Patterns Across Kingdomsmentioning

confidence: 99%

Pattern recognition receptors and signaling in plant–microbe interactions

2018

View full text Add to dashboard Cite

show abstract

“…Although the detection of the pathogen in plant tissues is improved by using polymerase chain reaction (PCR) methods compared with the isolation on growing media, quantification is still uncertain (Li et al 1994). Alternatively, real time quantitative PCR (qPCR) allows a simpler quantification, in addition to an increased sensitivity compared with conventional PCR assays (Ceccherini et al 2009;Luchi et al 2006;Mercado-Blanco et al 2003b). Sequences of the internal transcribed spacer region (ITS) of the ribosomal DNA (rDNA) are often used to design PCR primers in order to detect different fungal pathogens in host tissue, i.e., in potatoes (Mahuku et al 1999;Atallah et al 2007) and olive tissues (Markakis et al 2009;López-Escudero and Mercado-Blanco 2011).…”

Section: Introductionmentioning

confidence: 99%

Upward movement of Verticillium dahliae from soil to olive plants detected by qPCR

Ceccherini

Luchi

Pantani

et al. 2013

World J Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

Your article is protected by copyright and all rights are held exclusively by Springer Science +Business Media Dordrecht. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".

show abstract

Conversions and Pathways of Organic Carbon and Organic Nitrogen in Soils

Kalembasa

2016

Bioactive Compounds in Agricultural Soils

View full text Add to dashboard Cite

In‐field detection and quantification of extracellular DNA

Cited by 9 publications

References 17 publications

Pattern recognition receptors and signaling in plant–microbe interactions

Pattern recognition receptors and signaling in plant–microbe interactions

Upward movement of Verticillium dahliae from soil to olive plants detected by qPCR

Conversions and Pathways of Organic Carbon and Organic Nitrogen in Soils

Contact Info

Product

Resources

About