In Silico Signature Prediction Modeling in Cytolethal Distending Toxin-Producing Escherichia coli Strains

Abstract

In this study, cytolethal distending toxin (CDT) producer isolates genome were compared with genome of pathogenic and commensal Escherichia coli strains. Conserved genomic signatures among different types of CDT producer E. coli strains were assessed. It was shown that they could be used as biomarkers for research purposes and clinical diagnosis by polymerase chain reaction, or in vaccine development. cdt genes and several other genetic biomarkers were identified as signature sequences in CDT producer strains. The identified signatures include several individual phage proteins (holins, nucleases, and terminases, and transferases) and multiple members of different protein families (the lambda family, phage-integrase family, phage-tail tape protein family, putative membrane proteins, regulatory proteins, restriction-modification system proteins, tail fiber-assembly proteins, base plate-assembly proteins, and other prophage tail-related proteins). In this study, a sporadic phylogenic pattern was demonstrated in the CDT-producing strains. In conclusion, conserved signature proteins in a wide range of pathogenic bacterial strains can potentially be used in modern vaccine-design strategies.

Molecular exploring of multidrug resistance update of E.coli causing neonatal calves’ diarrhea.

“…E. coli can attain resistance genes by horizontal gene transfer (HGT) through interspecies and intra-species routes (Poirel et al 2018). The E. coli's HGT mechanisms include plasmids, transposons, and pathogenicity islands (PAIs) insertion into the recipient E. coli genome (Javadi et al 2017). Complicating this harmful scenario, E. coli can work not only as a recipient of HGT from other bacteria but also as a donor of resistance genes to other bacteria (Poirel et al, 2018), thus acting as an essential player in the widespread drug-resistance genes among pathogenic bacteria.…”

“…E. coli can attain resistance genes by horizontal gene transfer (HGT) through interspecies and intraspecies routes (Poirel et al 2018). The HGT mechanism varies, including plasmids, transposons, and pathogenicity islands (PAIs) insertion into the recipient E. coli genome (Javadi et al 2017). In this concern, controlling E. coli-induced neonatal diarrhea is still important.…”