<i>In situ</i> based surface-enhanced Raman spectroscopy (SERS) for the fast and reproducible identification of PHB producers in cyanobacterial cultures

Hermann, Daniel-Ralph; Lilek, David; Daffert, Christina; Fritz, Ines; Weinberger, Simone; Rumpler, Vanessa; Herbinger, Birgit; Prohaska, Katerina

doi:10.1039/d0an00969e

Cited by 10 publications

(6 citation statements)

References 26 publications

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“…This induced adhesion to the cell wall was also reported most recently for cyanobacteria. 50 The cell wall intrinsic SERS patterns that are achieved by label-free detection are employed for the simultaneous detection of different pathogens. 48,51,52 However, the sensitivity and validity of this label-free (direct) SERS-based approach is dependent on the SERS substrate, and this technique can be challenging in complex conditions, such as biofluid samples.…”

Section: Rational Design Of High-performance Sers Substratesmentioning

confidence: 99%

Surface-enhanced Raman spectroscopy for bioanalysis and diagnosis

et al. 2021

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Section: Rational Design Of High-performance Sers Substratesmentioning

confidence: 99%

Surface-enhanced Raman spectroscopy for bioanalysis and diagnosis

et al. 2021

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“…Raman spectroscopy has already been proven as a powerful tool for the characterization of PHB as it only requires small quantities of the sample and minimal sample preparation (Hermann et al, 2020). Analyses of Raman spectra are precise and less time-consuming.…”

Section: Raman Spectroscopymentioning

confidence: 99%

Poly-β-hydroxybutyrate (PHB) production potential of naturally existing cyanobacterial blooms

Wijerathne

Manage

Idroos

2022

J. Natn. Sci. Foundation Sri Lanka

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Detrimental effects imposed by petrochemical plastics on the environment are among the most often discussed concerns in the current era. Although the scientific community has discovered numerous eco-friendly alternatives, the cost of manufacture has restricted the usage of such material. The present study focused on the direct utilization of naturally existing cyanobacterial blooms to extract biodegradable poly-β-hydroxybutyrate (PHB) aiming to minimize the cost of production by eliminating the need for growing of cyanobacterial monocultures. This attempt provides remedies for plastic pollution and hazardous cyanobacterial blooms simultaneously. Fresh cyanobacterial bloom samples were collected from the hypereutrophic Beira Lake, Colombo. They were maintained under in vitro conditions for 7 days, provided with a 12/12 hours light/dark cycle, and deprived of an external supply of nutrients. PHB production was optimized for Nitrogen, Phosphate, and Carbon sources: glucose, sucrose, and lactose. The extracted PHB was quantified by spectrophotometric analysis. Structure confirmation was carried out using FTIR and Raman spectroscopy. The mean percentage weight of PHB yield was 7.13 ± 0.12% w/w. Optimization studies showed that nitrate deficiency and the presence of glucose as an exogenous carbon source imposed a stimulatory effect for PHB accumulation by cyanobacteria. The maximum amount of PHB (9.6% w/w) was found to be accumulated by cyanobacteria on the fourth day following the bloom sample collection. Hence, the present study proposes a sustainable utilization method of cyanobacterial blooms as a promising source for PHB production.

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“…[ 200 ] Crotonic acid, produced by sulfuric acid treatment of PHB, can be determined with HPLC. [ 201,202 ] PHA copolymer composition has successfully been verified in HPLC with alkali‐hydrolyzed samples. [ 203 ] Monomeric diols, following reductive depolymerization of mcl‐ PHA, can also be measured by HPLC/MS as a simple, fast alternative to that in 1 H NMR.…”

Section: Identification Quantification and Characterization Of Phamentioning

confidence: 99%

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

Lhamo

Behera

Mahanty

2021

Biotechnology Journal

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Background Microbial polyhydroxyalkanoates (PHAs) produced using renewable resources could be the best alternative for conventional plastics. Despite their incredible potential, commercial production of PHAs remains very low. Nevertheless, sincere attempts have been made by researchers to improve the yield and economic viability of PHA production by utilizing low‐cost agricultural or industrial wastes. In this context, the use of efficient microbial culture or consortia, adoption of experimental design to trace ideal growth conditions, nutritional requirements, and intervention of metabolic engineering tools have gained significant attention. Purpose and scope This review has been structured to highlight the important microbial sources for PHA production, use of conventional and non‐conventional substrates, product optimization using experimental design, metabolic engineering strategies, and global players in the commercialization of PHA in the past two decades. The challenges about PHA recovery and analysis have also been discussed which possess indirect hurdle while expanding the horizon of PHA‐based bioplastics. Summary Selection of appropriate microorganism and substrate plays a vital role in improving the productivity and characteristics of PHAs. Experimental design‐based bioprocess, use of metabolic engineering tools, and optimal product recovery techniques are invaluable in this dimension. Conclusion Optimization strategies, which are being explored in isolation, need to be logically integrated for the successful commercialization of microbial PHAs.

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In situ based surface-enhanced Raman spectroscopy (SERS) for the fast and reproducible identification of PHB producers in cyanobacterial cultures

Abstract:
Reproducible in situ SERS delivers a significantly reduced analysis time compared to HPLC, allowing timely decisions regarding Synechocystis PHB production.

Cited by 10 publications

References 26 publications

Surface-enhanced Raman spectroscopy for bioanalysis and diagnosis

Surface-enhanced Raman spectroscopy for bioanalysis and diagnosis

Poly-β-hydroxybutyrate (PHB) production potential of naturally existing cyanobacterial blooms

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

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In situ based surface-enhanced Raman spectroscopy (SERS) for the fast and reproducible identification of PHB producers in cyanobacterial cultures

Abstract: Reproducible in situ SERS delivers a significantly reduced analysis time compared to HPLC, allowing timely decisions regarding Synechocystis PHB production.

Cited by 10 publications

References 26 publications

Surface-enhanced Raman spectroscopy for bioanalysis and diagnosis

Surface-enhanced Raman spectroscopy for bioanalysis and diagnosis

Poly-β-hydroxybutyrate (PHB) production potential of naturally existing cyanobacterial blooms

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

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Resources

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Abstract:
Reproducible in situ SERS delivers a significantly reduced analysis time compared to HPLC, allowing timely decisions regarding Synechocystis PHB production.