In Situ Biodiesel Production from Fast‐Growing and High Oil Content Chlorella pyrenoidosa in Rice Straw Hydrolysate

Abstract

Rice straw hydrolysate was used as lignocellulose-based carbon source for Chlorella pyrenoidosa cultivation and the feasibility of in situ biodiesel production was investigated. 13.7 g/L sugar was obtained by enzymatic hydrolyzation of rice straw. Chlorella pyrenoidosa showed a rapid growth in the rice straw hydrolysate medium, the maximum biomass concentration of 2.83 g/L was obtained in only 48 hours. The lipid content of the cells reached as high as 56.3%. In situ transesterification was performed for biodiesel production. The optimized condition was 1 g algal powder, 6 mL n-hexane, and 4 mL methanol with 0.5 M sulfuric acid at the temperature of 90°C in 2-hour reaction time, under which over 99% methyl ester content and about 95% biodiesel yield were obtained. The results suggested that the method has great potential in the production of biofuels with lignocellulose as an alternative carbon source for microalgae cultivation.

Isolation and Characterization of Native Microalgae from the Peruvian Amazon with Potential for Biodiesel Production

“…Microalgae species of these genera were also isolated from different countries of the world, such as Brazil [47], Argentina [46], United States [48], India [20], Egypt [49], Taiwan [50], South Korea [45] and China [51]. Some of these microalgae genera, such as Ankistrodesmus, Chlorella, Desmodesmus and Scenedesmus, were used in large-scale biodiesel production using photobioreactors and raceway pond systems [52,53]. public GenBank database.…”

Effects of Light Exposure and Nitrogen Source on the Production of Oil from Freshwater and Marine Water Microalgae

“…Fried et al (2003) stated a significant positive effect on algae growth with both nitrogen and phosphorus as nutrient sources. Li et al (2011) noted that the species Scenedesmus sp. produced a higher cell productivity of 2.21×10 6 cells/mL/d with nitrogen and phosphorous contents of 12.1 and 0.27 mg/L, respectively.…”

Biodiesel Production from Scenedesmus sp. through Optimized in situ Acidic Transesterification Process

“…[1][2][3] Extensive study has been conducted on using edible oils, which limited by the availability of oil inventories, but these materials result in high sensitivity of prices to oil demand from industry. [4][5][6][7] In order to not compete with edible oils, the lowcost and profitable biodiesel should be produced from low-cost feedstocks such as non-edible oils (used frying oils, animal fats and greases etc.). However the available quantities of waste oils and animal fats are not enough to match the today demands for biodiesel.…”

“…8 In addition, liquid culture of the microalgae can be controlled easily and even used for a waste treatment as a new source having renewable, environmental and economical sustainability. 4,6,[9][10][11] However, in making biodiesel, actually Fatty Acid Methyl Esters (FAME), generally lipid extraction and its transesterification process produce large amount of hazardous solvent waste and are also very cumbersome. 6,12,13 Automated extraction equipment such as the Soxhlet apparatus has been designed, but they require the long time of extraction process.…”

“…4,6,[9][10][11] However, in making biodiesel, actually Fatty Acid Methyl Esters (FAME), generally lipid extraction and its transesterification process produce large amount of hazardous solvent waste and are also very cumbersome. 6,12,13 Automated extraction equipment such as the Soxhlet apparatus has been designed, but they require the long time of extraction process. To overcome this limitation, one step to make biodiesel, in situ transesterification (or direct transesterification from the biomass) process, has been considered where intact biomas rather than pre-extracted oil directly contacts with acidified or alkalized alcohol that acts as both an extraction solvent and an esterification reagent.…”