In situ bioimaging of Lactobacillus by photoluminescence of MoS₂

Abstract

Due to unique properties arising from its 2D nature, molybdenum disulfide (MoS2) has been studied widely toward its application for biosensing. While MoS2 field effect transistor has been utilized for electrical detection of biological events in many studies, photoluminescence (PL) characteristics of MoS2 have been poorly employed. MoS2 PL can provide not only information of interactions between biological moieties and the surface, but also their spatial distribution. In this work, we utilized PL of single-layer MoS2 as a label-free bioimaging sensor. To fabricate the imaging device, we synthesized MoS2 by chemical vapor deposition and transferred it on a glass substrate with patterned electrodes. We employed Lactobacillus known as a probiotic microorganism to demonstrate the ability of bioimaging. We found that the MoS2 PL can be modulated by the concentration of lactic acid. Finally, we succeeded in monitoring PL image of MoS2 modulated by Lactobacillus, which can be correlated with the production of lactic acid by Lactobacillus.

Resolution of MoS2 Nanosheets‐Induced Pulmonary Inflammation Driven by Nanoscale Intracellular Transformation and Extracellular‐Vesicle Shuttles

“…Inorganic 2D materials particularly transition metal dichalcogenides (TMDs) have raised significant attention due to their exceptional electronic, optical, mechanical, and chemical properties. [1,2] Notably, the large surface area with tunable electronic properties, the intercalable layers and biofunctionalization, holds great promise in biosensing, [3][4][5] bioimaging, [6,7] and drug delivery, [8][9][10] among others applications. [11] In particular, molybdenum disulfide, a graphene analogue consisting of SMoS covalent arrangements [12] is one of the commercially attractive materials used as catalyst, lubricant, welding, and combustion.…”

Self-assembled GA-Repeated Peptides as a Biomolecular Scaffold for Biosensing with MoS2 Electrochemical Transistors

“…Electrical detection of the target molecules with transistor-based biosensors is currently a ubiquitous mechanism for biosensing. To enhance the transistor-based biosensors’ sensitivity, nanomaterials such as Si nanowires and carbon nanotubes are often used for the detection-channel part of the transistor because of their high specific surface area. − More recently, biosensors with two-dimensional (2D) nanomaterials represented by graphene and MoS 2 have demonstrated a high sensitivity to a variety of target molecules. − While graphene is semi-metallic, MoS 2 is a new member of the 2D materials with a semiconducting property, allowing for higher electrical sensitivity for biosensing, in principle, than graphene biosensors …”

“…The aligned structures have been proven to be stable under an aqueous solution and even under an applied electrical bias . Furthermore, biosensing has been demonstrated by immobilizing bioprobes supported by self-assembled peptides on graphene/graphite surfaces in a controlled manner. , Because of increasing interest in the application of MoS 2 for biosensing, ,, it is essential to unravel the electronic interaction of self-assembled peptides and MoS 2 and their ability as a molecular scaffold for biosensing.…”

Resolution of MoS2 nanosheets-induced pulmonary inflammation driven by nanoscale intracellular transformation and extracellular-vesicle shuttles

“…Inorganic two-dimensional (2D) materials particularly transition metal dichalcogenides (TMDs) have raised significant attention due to their exceptional electronic, optical, mechanical, and chemical properties. [1,2] Notably, the large surface area with tunable electronic properties, the intercalable layers and bio-functionalization holds great promise in biosensing, [3][4][5] bioimaging, [6,7] and drug delivery, [8][9][10] among others applications. [11] In particular, molybdenum disulfide, a graphene analogue consisting of S-Mo-S covalent arrangements [12] is one of the commercially attractive materials used as catalyst, lubricant, welding, and combustion.…”