In SituBiosynthesis of a Metal Nanoparticle Encapsulated in Alginate Gel for Imageable Drug-Delivery System

Abstract

Development of drug-delivery systems that allow simultaneous in vivo imaging has gained much interest. We report a novel strategy to encapsulate metal nanoparticles (NPs) within alginate gel for in vivo imaging. The cell lysate of recombinant Escherichia coli strain, expressing Arabidopsis thaliana phytochelatin synthase and Pseudomonas putida metallothionein genes, was encapsulated within the alginate gel. Incubation of alginate gel with metal ion precursors followed by UV irradiation resulted in the synthesis of high concentrations of metal NPs, such as Au, Ag, CdSe, and EuSe NPs, within the gel. The alginate gel with metal NPs was used as a drug-delivery system by further co-encapsulating doxorubicin and rifampicin, the release of which was made to be pH-dependent. This system can be conveniently and safely used for in vitro and in vivo bioimaging, enabled by the metal NPs formed within the gel matrix without using toxic reducing reagents or surfactants.

Peroxidase-Mimicking Activity of Biogenic Gold Nanoparticles Produced from Prunus nepalensis Fruit Extract: Characterizations and Application for the Detection of Mycobacterium bovis

“… 15 Additionally, new breakthroughs in biogenic synthesis of nanomaterials have allowed for applications beyond catalysis and biosensing, for example, applications in drug delivery and in bioimaging have been reported. 16 …”

“…Similarly, Li and co-workers synthesized stable Au NPs using kiwi extract with higher stability and intrinsic peroxidase-mimicking activity for the colorimetric detection of cysteine . Additionally, new breakthroughs in biogenic synthesis of nanomaterials have allowed for applications beyond catalysis and biosensing, for example, applications in drug delivery and in bioimaging have been reported …”

Material and Design Toolkit for Drug Delivery: State of the Art, Trends, and Challenges

“…Copyright 2021 American Chemical Society. (d) To create an imageable drug delivery system, a schematic representation of the in vitro synthesis of metal nanoparticles in alginate gel is shown, Reproduced with permission from ref, Copyright 2021, ACS. e. The synthesis of DA-NCPs from DA, Fe (AcO)­2, and BIX is shown schematically.…”

Gold nanoparticles supported on ionic‐liquid‐functionalized cellulose (Au@CIL): A heterogeneous catalyst for the selective reduction of aromatic nitro compounds

“…Because of its low cost, high oxygen content, and low greenhouse gas emissions, it can be used in the pharmaceutical industry, biofuels, and organic synthesis. [29,30] Because of the spherical and porous beads of cellulose, its catalytic efficacy can be significantly increased by embedding metal NPs. [28] Moreover, cellulose can be applied more widely in a chemically modified hydrophobic state.…”