<i>In situ</i> grown magnetic COF@MOF with a phosphoserine anchor for in-depth <i>N</i>-glycopeptide analysis in serum

Guo, Yimin; Hua, Shuwen; Wang, Baichun; Wang, Bing; Ding, Chuan-Fan; Yan, Yinghua

doi:10.1039/d3an01473h

Cited by 7 publications

(1 citation statement)

References 47 publications

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“…Glycoproteins play crucial roles in many fundamental physiological and disease processes, such as intercellular interactions, immune responses, and tumor development. , The glycosylated proteins occupy over 50% of human proteins, and the abnormal glycosylation patterns of proteins are closely related to the origin and progress of a number of diseases, like cancer, autoimmune disorders, cardiovascular diseases, and neurodegenerative diseases. So many glycoproteins have been regarded as biomarkers in clinical diagnosis. − The current methods to detect glycoproteins mainly rely on mass spectroscopy and enzyme-linked immunosorbent assays, which are either expensive or time-consuming. Moreover, skilled personnel and centralized instruments are typically needed to perform these measurements.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Impedimetric Platform Based on Con A@MIL-101 for Glycoprotein Detection

Li,

2024

Langmuir

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An electrochemical impedimetric biosensing platform with lectin as a molecular recognition element has been established for the sensitive detection of glycoproteins, a class of important biomarkers in clinical diagnosis. One of the representative metal−organic framework materials, MIL-101(Cr)-NH 2 , was utilized as the supporting matrix, and its amino groups served as the anchors to immobilize the lectins of concanavalin A (Con A), constituting Con A@MIL-101(Cr)-NH 2 for the determination of invertase (INV) as a model glycoprotein. The Con A concentration, immobilization time, and incubation time with INV were optimized. Under the optimal conditions, the degree of impedance increase was linearly proportional to the logarithm of INV concentration between 1.0 × 10 −16 and 1.0 × 10 −11 M, affording a limit of detection as low as 3.98 × 10 −18 M. Good specificity, stability, reproducibility, and repeatability were demonstrated for the fabricated biosensing platform. Moreover, real mouse serum samples were spiked with different concentrations of INV. Excellent recoveries were obtained, which demonstrated the biosensing platform's capability of analyzing glycoproteins within a complex matrix.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Impedimetric Platform Based on Con A@MIL-101 for Glycoprotein Detection

Li,

2024

Langmuir

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Hydrophilic materials based on hydrogel polymers for enrichment of glycopeptides from serum of colorectal cancer patients

Zhang,

Wang,

Shao

et al. 2024

J of Separation Science

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In this work, a composite hydrogel material consisting of chitosan‐based composite hydrogel was prepared by a simple and rapid synthetic method and will be named three‐dimensional (3D)‐IL‐COF‐1@CS hydrogel. Possessing a stable 3D network structure and outstanding hydrophilicity, the novel hydrogel is capable of capturing glycopeptides. The 3D‐IL‐COF‐1@CS hydrogel showed good sensitivity (0.1 fmol/µL) and selectivity (1:2000). In addition, 19 glycopeptides were captured in standard samples. In the analysis of human serum, 148 glycopeptides assigned to 72 glycoproteins were assayed in the serum of normal individuals, and 245 glycopeptides corresponding to 100 glycoproteins were found in the serum of colorectal cancer (CRC) patients. More importantly, several functional programs based on Gene Ontology analysis supported molecular biological processes that may be relevant to the pathogenesis of CRC, including aging, fibrinogen complex, and arylesterase activity. The low cost, simplicity, rapid synthesis, and good enrichment performance have a great future in glycoproteomics analysis and related diseases.

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Exploitation of porphyrin-based titanium-rich porous organic polymers for targeted phosphopeptide enrichment from the serum of colorectal cancer individuals

Wang,

Sheng,

Shao

et al. 2024

Microchim Acta

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In situ grown magnetic COF@MOF with a phosphoserine anchor for in-depth N-glycopeptide analysis in serum

Abstract: A hydrophilic phosphoserine-functionalized magnetic organic framework composite (termed Fe3O4@COF@MOF-PS) was synthesized by in situ growth strategy for effective capture of N-glycopeptides. Fe3O4@COF@MOF-PS exhibited high sensitivity (0.2 fmol μL-1), outstanding exclusion...

Cited by 7 publications

References 47 publications

Electrochemical Impedimetric Platform Based on Con A@MIL-101 for Glycoprotein Detection

Electrochemical Impedimetric Platform Based on Con A@MIL-101 for Glycoprotein Detection

Hydrophilic materials based on hydrogel polymers for enrichment of glycopeptides from serum of colorectal cancer patients

Exploitation of porphyrin-based titanium-rich porous organic polymers for targeted phosphopeptide enrichment from the serum of colorectal cancer individuals

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