<i>In situ</i> lipid dip‐pen nanolithography under water

Lenhert, Steven; Mirkin, Chad A.; Fuchs, Harald

doi:10.1002/sca.20166

Cited by 29 publications

(24 citation statements)

References 64 publications

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“…There are many techniques to create SLBs, including Langmuir-Blodgett/Schäfer deposition,[1] spin coating,[2] microcontact printing,[3] solvent-exchange deposition,[4] lipid-surfactant micelles,[5] evaporation induced assembly,[6] bubble collapse deposition,[7*] lipid dip-pen nanolithography,[8] and vesicle fusion. [9*] Langmuir-Blodgett/Schäfer (LB/LS) deposition and vesicle fusion are perhaps the most commonly used techniques to form SLBs.…”

Section: Introductionmentioning

confidence: 99%

Model cell membranes: Techniques to form complex biomimetic supported lipid bilayers via vesicle fusion

Hardy

Nayak

Zauscher

2013

Current Opinion in Colloid & Interface Science

194

183

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Vesicle fusion has long provided an easy and reliable method to form supported lipid bilayers (SLBs) from simple, zwitterionic vesicles on siliceous substrates. However, for complex compositions, such as vesicles with high cholesterol content and multiple lipid types, the energy barrier for the vesicle-to-bilayer transition is increased or the required vesicle-vesicle and vesicle-substrate interactions are insufficient for vesicle fusion. Thus, for vesicle compositions that more accurately mimic native membranes, vesicle fusion often fails to form SLBs. In this paper, we review three approaches to overcome these barriers to form complex, biomimetic SLBs via vesicle fusion: (i) optimization of experimental conditions (e.g., temperature, buffer ionic strength, osmotic stress, cation valency, and buffer pH), (ii) α-helical (AH) peptide-induced vesicle fusion, and (iii) bilayer edge-induced vesicle fusion. AH peptide-induced vesicle fusion can form complex SLBs on multiple substrate types without the use of additional equipment. Bilayer edge-induced vesicle fusion uses microfluidics to form SLBs from vesicles with complex composition, including vesicles derived from native cell membranes. Collectively, this review introduces vesicle fusion techniques that can be generalized for many biomimetic vesicle compositions and many substrate types, and thus will aid efforts to reliably create complex SLB platforms on a range of substrates.

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Section: Introductionmentioning

confidence: 99%

Model cell membranes: Techniques to form complex biomimetic supported lipid bilayers via vesicle fusion

Hardy

Nayak

Zauscher

2013

Current Opinion in Colloid & Interface Science

194

183

View full text Add to dashboard Cite

show abstract

“…The study of these systems showed the presence of liquid disordered, liquid ordered and gel phases in dry lipid samples, with morphological properties (e.g. layer thicknesses) in similarity to those observed on the same systems under hydrated conditions and prepared by different techniques such as, the vesicle fusion [23], Langmuir-Blodgett/Schäfer [24], microcontact printing [25] or lipid dippen nanolithography [26].…”

Section: Introductionmentioning

confidence: 64%

Interdigitation in spin-coated lipid layers in air

Dols-Perez

Fumagalli

Gomila

2018

Colloids and Surfaces B: Biointerfaces

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In this study, we show that dry saturated phospholipid layers prepared by the spin-coating technique could present thinner regions associated to interdigitated phases under some conditions. The morphological characteristics of lipid layers of saturated phosphocholines, such as dilauroylphosphatidylcholine (DLPC), dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC) and distearoylphosphatidylcholine (DSPC), have been measured by Atomic Force Microscopy and revealed that the presence of interdigitated regions is not induced by the same parameters that induce them in hydrated samples. To achieve these results the effect of the lipid hidrocabonated chain length, the presence of alcohol in the coating solution, the spinning velocity and the presence of cholesterol were tested. We showed that DPPC and DSPC bilayers, on the one side, can show structures with similar height than interdigitated regions observed in hydrated samples, while, on the other side, DLPC and DMPC tend to show no evidence of interdigitation. Results indicate that the presence of interdigitated areas is due to the presence of lateral tensions and, hence, that they can be eliminated by releasing these tensions by, for instance, the addition of cholesterol. These results demonstrate that interdigitation in lipid layers is a rather general phenomena and can be observed in lipid bilayers in dry conditions.

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“…Phospholipids are a particularly useful as inks for DPN because of their physicochemical properties as well as innate biofunctionality. (1)(2)(3) Here the dynamic adhesion properties of lipid multilayer structures fabricated by DPN is studied. Short-timescale kinetics of lipid spreading and other shape changes within the micro-and nanostructured lipid multilayers is studied.…”

Section: -Pos Board B745 Adhesion Of Lipid Multilayer Micro-and Nmentioning

confidence: 99%

Lipid Membrane Domains Promote In-Vitro Presynapse Formation

Gopalakrishnan¹,

Yam²,

Rouiller³

et al. 2011

Biophysical Journal

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Regeneration of damaged neurons has been one of the main challenges in the attempt to cure brain damages occur from neurodegenerative diseases and head injuries. Successful formation of functional synapses on artificial substrates is a very important step in the development of engineered in vitro neural networks. We have recently shown that spherical supported bilayer lipid membranes (SS-BLMs) can be used as a novel substrate to achieve presynaptic accumulation at an in vitro synaptic junction. 1 The results indicate that lipid membrane domains may play a role in the observed phenomenon, in addition to the chemical and electrostatic interactions between the neurons and SS-BLMs. 2 With the help of domain specific fluorescent dyes, micron-sized lipid phase domains were specifically labeled on Giant Unilamellar Vesicles (GUVs) and on silica beads. Experiments, in vitro, clearly show that specific membrane domains play a role in the synapse formation. These results as well as Cryo-TEM based molecular level confirmation of presynapse formation on artificial substrates will be presented.The aspect of lipid membrane domains in synapse formation is key in developing many neuroengineering approaches to design functional artificial synapse formation as well as for synaptogenesis studies in vivo. 1. Lipid Bilayer Membrane-Triggered Presynaptic Vesicle Assembly'' Gopa

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In situ lipid dip‐pen nanolithography under water

Cited by 29 publications

References 64 publications

Model cell membranes: Techniques to form complex biomimetic supported lipid bilayers via vesicle fusion

Model cell membranes: Techniques to form complex biomimetic supported lipid bilayers via vesicle fusion

Interdigitation in spin-coated lipid layers in air

Lipid Membrane Domains Promote In-Vitro Presynapse Formation

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