In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas

Abstract

Efficient manufacturing of increasingly sophisticated biopharmaceuticals requires the development of new breeds of chromatographic materials featuring two or more layers, with each layer affording different functions. This letter reports the in situ modification of a commercial beaded anion exchange adsorbent using atmospheric pressure plasma generated within gas bubbles. The results show that exposure to He-O2 plasma in this way yields significant reductions in the surface binding of plasmid DNA to the adsorbent exterior, with minimal loss of core protein binding capacity; thus, a bi-layered chromatography material exhibiting both size excluding and anion exchange functionalities within the same bead is produced.

Temporal evolution of the relative vibrational population of N2 (C3∏u) and optical emission spectra of atmospheric pressure plasma jets in He mixtures

“…Atmospheric pressure plasma jets (APPJs) have attracted lots of interest because of their wide applications in material processing [1][2][3], surface modification [4,5], chemical reaction engineering [6,7], and especially in biological medicine [8][9][10][11][12]. As a kind of extreme non-equilibrium plasma, APPJs can generate plasmas in confinement of electrodes and di electric tubes, and then propagate into open air.…”

Marangoni flows induced by atmospheric-pressure plasma jets

“…Atmospheric-pressure plasma jets [1,2] have generated significant interest for their versatile applications in material processing [1][2][3][4], surface modification [5][6][7][8], medicine [9][10][11][12][13][14][15][16][17][18][19][20][21] and chemical reaction engineering [22][23][24][25]. Fricke et al studied the etching of polymers using atmosphericpressure Ar plasma jets with different oxygen admixtures and found etchrates in the order of 20 nm s −1 for polyethylene [4].…”