<i>In situ</i>monitoring of protein aggregation<i>via</i>clusteroluminescence

Zhang, Zhiming; Zhu, Longchen; Feng, Jianghua; Zhang, Haoke; Zhang, Xin; Sun, Jing; Tang, Benzhong

doi:10.1039/d2qm01032a

Cited by 16 publications

(11 citation statements)

References 39 publications

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“…The maximum intensity is observed when protein dimers begin to lose their original conformation. During aggregation, the fluorescence intensity decreases mainly due to the screening of some molecules by others [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

pH-Dependent HEWL-AuNPs Interactions: Optical Study

Molkova,

Pustovoy,

Stepanova

et al. 2023

Molecules

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Optical methods (spectroscopy, spectrofluorometry, dynamic light scattering, and refractometry) were used to study the change in the state of hen egg-white lysozyme (HEWL), protein molecules, and gold nanoparticles (AuNPs) in aqueous colloids with changes in pH, and the interaction of protein molecules with nanoparticles was also studied. It was shown that changing pH may be the easiest way to control the protein corona on gold nanoparticles. In a colloid of nanoparticles, both in the presence and absence of protein, aggregation–deaggregation, and in a protein colloid, monomerization–dimerization–aggregation are the main processes when pH is changed. A specific point at pH 7.5, where a transition of the colloidal system from one state to another is observed, has been found using all the optical methods mentioned. It has been shown that gold nanoparticles can stabilize HEWL protein molecules at alkaline pH while maintaining enzymatic activity, which can be used in practice. The data obtained in this manuscript allow for the state of HEWL colloids and gold nanoparticles to be monitored using one or two simple and accessible optical methods.

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Section: Discussionmentioning

confidence: 99%

pH-Dependent HEWL-AuNPs Interactions: Optical Study

Molkova,

Pustovoy,

Stepanova

et al. 2023

Molecules

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“…The similar phenomenon has also been observed in natural polymers, including rice, starch, cellulose, [ 13 ] and proteins. [ 14 ] These non‐ conjugated structures do not fluoresce in isolated state but show anomalous fluorescence upon aggregation, known as clusteroluminescence (CL). [ 15‐21 ] Luminogens with CL (CLgens) hold significant technological, theoretical, and practical value.…”

Section: Background and Originality Contentmentioning

confidence: 99%

Emerging Clusteroluminescence from Complexes between Carbonyl‐Based Polymers and Organic Base^†

Xie

Zhang

et al. 2023

Chin. J. Chem.

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Comprehensive SummaryThe study of luminescence phenomena in non‐conjugated systems, namely clusteroluminescence, has gained significant attention for the development of advanced luminescent materials. While conventional strategies to manipulate the luminescent performances are based on complicated chemical reactions. In contrast, nature employs complexation to modulate luminescence, inspiring researchers to adopt an engineering approach for the construction of efficient clusteroluminogens. In this work, we explore the complexation‐induced clusteroluminescence of carbonyl‐based polymers with nitrogen‐containing organic bases, exemplified by polyamide, polyester, polycarbonate, and poly(monothiocarbonate). The results demonstrate an increase in the intrinsic 440 nm emission of carbonyl groups and the emergence of new emission peaks upon complexation. The study proposes a through‐space n‧‧‧π complex mechanism, highlighting the potential of complexation as a strategy for modulating the clusteroluminescent properties of non‐conjugated systems. Further research is necessary to unravel underlying mechanisms, optimize cluster structures, and explore new materials for complexation, thereby advancing optoelectronics and photonics fields and enabling practical applications of clusteroluminescent materials.This article is protected by copyright. All rights reserved.

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“…Hence, the investigation of CL is of great theoretical significance and versatile advantages in biological applications. [14] Nevertheless, most reported CLgens can only emit blue or green light, and it is a great challenge to fabricate red-emission CLgens, let alone CLgens with nearinfrared (NIR) emission, which is ascribed to the weak TSIs and unclear structure-property relationship. Therefore, the manipulation of CL behaviors and constructing red/NIRemission CLgens are urgent problems in this area.…”

Section: Introductionmentioning

confidence: 99%

NIR Clusteroluminescence of Non‐conjugated Phenolic Resins Enabled by Through‐Space Interactions

Zhang

Xiong

et al. 2023

Angewandte Chemie

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Clusteroluminescence (CL) and through-space interactions (TSIs) of non-conjugated molecules have drawn more attention due to their unique photophysical behaviors that are different from largely conjugated luminogens. However, achieving red and even near-infrared (NIR) emission from such systems is still challenging due to the intrinsic drawbacks of non-conjugated molecules and the lack of theories for structure-property relationships. In this work, six phenolic resins are designed and synthesized based on two molecule-engineering strategies: increasing the number of TSIs units and introducing electron-donating/-withdrawing groups. All phenolic resins are verified as luminogens with CL property (CLgens), and the first example of CLgens with NIR emission (maximum emission wavelength � 680 nm) and high absolute quantum yield (47 %) is reported. Experiments and theoretical analysis reveal that two TSIs types, through-space locally excited state and through-space charge transfer state, play essential roles in achieving CL from these non-conjugated polymers, which could be manipulated via changing structural conformation and electron density or altering electron transition behaviors. This work not only provides an approach to manipulate TSIs and CL of nonconjugated polymers but also endows commercially available phenolic resins with high practical value as luminescence materials.

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In situmonitoring of protein aggregationviaclusteroluminescence

Abstract: Proteins, showing non-conjugated and nonaromatic structures, have been discovered with abnormal visible emission at the clustering state, namely clusteroluminescence (CL). Exploring the practical application of CL is one of the...

Cited by 16 publications

References 39 publications

pH-Dependent HEWL-AuNPs Interactions: Optical Study

pH-Dependent HEWL-AuNPs Interactions: Optical Study

Emerging Clusteroluminescence from Complexes between Carbonyl‐Based Polymers and Organic Base^†

NIR Clusteroluminescence of Non‐conjugated Phenolic Resins Enabled by Through‐Space Interactions

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In situmonitoring of protein aggregationviaclusteroluminescence

Abstract: Proteins, showing non-conjugated and nonaromatic structures, have been discovered with abnormal visible emission at the clustering state, namely clusteroluminescence (CL). Exploring the practical application of CL is one of the...

Cited by 16 publications

References 39 publications

pH-Dependent HEWL-AuNPs Interactions: Optical Study

pH-Dependent HEWL-AuNPs Interactions: Optical Study

Emerging Clusteroluminescence from Complexes between Carbonyl‐Based Polymers and Organic Base†

NIR Clusteroluminescence of Non‐conjugated Phenolic Resins Enabled by Through‐Space Interactions

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Product

Resources

About

Emerging Clusteroluminescence from Complexes between Carbonyl‐Based Polymers and Organic Base^†