In situpreparation of programmable curli nanomaterials as fine-tuned sustainable supports enabling selective and oriented incorporation of enzymes

Abstract

Pollution caused by phthalic acid esters (PAEs) has shown interference with hormone homeostasis. To reduce the influence of PAEs on the environment, a two-step method for magnetic curli nanomaterial preparation...

Genetically Decorating Microbial Surface Curli Fiber with Biocatalytic Properties

“…Curli, the primary proteinaceous component of biofilms of Escherichia coli, serves as a functional adhesive amyloid . This protein can act as a protein scaffold, enabling the synthesis of diverse hybrid biomaterials. − The Mr cp19k protein, the most crucial interface adhesion protein in barnacle cement, plays a crucial role in ensuring the surface coupling of the cement on the substrate. , SpyCatcher and SpyTag have the ability to form a spontaneous and irreversible isopeptide bond, serving as a powerful tool for bioconjugation and creating protein architecture through covalent interactions. − Although there are a few limited instances of immobilizing α-amylase, threonine aldolase and esterase using curli-SpyCatcher/SpyTag for their respective catalytic reactions, − the need for a new functional module persists to further enrich technological advancements and enhance biocatalytic performances. While barnacles utilize self-assembled amyloids in the design of biomimetic adhesives, , their application in developing biocatalysis systems remains underutilized.…”