<i>In situ</i> tissue pathology from spatially encoded mass spectrometry classifiers visualized in real time through augmented reality

Woolman, Michael; Qiu, Jimmy; Kuzan-Fischer, Claudia M.; Ferry, Isabelle; Dara, Delaram; Katz, Lauren; Daud, Fowad; Wu, Megan; Ventura, Manuela; Bernards, Nicholas; Chan, Harley; Fricke, Inga B.; Zaidi, Mark; Wouters, Brad; Rutka, James T.; Das, Sunit; Irish, Jonathan C.; Weersink, Robert A.; Ginsberg, Howard J.; Jaffray, David A.; Zarrine‐Afsar, Arash

doi:10.1039/d0sc02241a

Cited by 25 publications

(24 citation statements)

References 47 publications

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“…In this way, the automated robot-assisted system will move and screen the desired position indicated by the medical staff and obtained a real-time feedback that could be monitored directly by the medical team though augmented-reality. Some efforts have already been made in this direction by the preliminary in situ analysis . While the available intraoperative hand-held MS devices have proven useful in analysis of numerous cancer tissues, margin delineation and debulking efficiency remain scarce.…”

Section: Conclusion and Perspectivementioning

confidence: 99%

Robot-Assisted SpiderMass for In Vivo Real-Time Topography Mass Spectrometry Imaging

et al. 2021

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Mass Spectrometry Imaging (MSI) has shown to bring invaluable information for biological and clinical applications. However, conventional MSI is generally performed ex vivo from tissue sections. Here, we develop a novel MS-based method for in vivo mass spectrometry imaging. By coupling the SpiderMass technology -that provides in vivo minimally invasive analysis -to a robotic arm of high accuracy, we demonstrate that images can be acquired from any surface by moving the laser probe above the surface. By equipping the robotic arm with a sensor, we are also able to both get the topography image of the sample surface and the molecular distribution, and then and plot back the molecular data, directly to the 3D topographical image without the need for image fusion. This is shown for the first time with the 3D topographic MS-Based whole-body imaging of a mouse. Enabling fast in vivo MSI bridged to topography pave the way for surgical applications to excision margins.

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Section: Conclusion and Perspectivementioning

confidence: 99%

Robot-Assisted SpiderMass for In Vivo Real-Time Topography Mass Spectrometry Imaging

et al. 2021

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“…Finally, the future vision for surgery 2.0 is certainly based on autonomous robotic devices for guidance of the MS probe in minimally invasive surgery. Such systems are foreseen to be coupled to augmented reality, as the PIRL-MS in preliminary ex vivo experiments with the optical surgical tracking system [88], to provide surgeons with the right decision-making. While there are still many roadblocks ahead before the probes can be widely accepted as medical tools in routine clinical use, the recently developed MS probes are moving toward rapid, ease-of use, and costeffective solutions for improved patient care.…”

Section: Discussionmentioning

confidence: 99%

Cancer Surgery 2.0: Guidance by Real-Time Molecular Technologies

Ogrinc

Saudemont

Takáts

et al. 2021

Trends in Molecular Medicine

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In vivo cancer margin delineation during surgery remains a major challenge. Despite the availability of several image guidance techniques and intraoperative assessment, clear surgical margins and debulking efficiency remain scarce. For this reason, there is particular interest in developing rapid intraoperative tools with high sensitivity and specificity to help guide cancer surgery in vivo. Recently, several emerging technologies including intraoperative mass spectrometry have paved the way for molecular guidance in a clinical setting. We evaluate these techniques and assess their relevance for intraoperative surgical guidance and how they can transform the future of molecular cancer surgery, diagnostics, patient management and care. HighlightsCancer surgery is the first treatment in many solid tumors. Quality of the initial surgery has a strong influence on the patient outcome. Tumors must be excised at best, but accurate delineation of their edges and margin definition is a huge challenge.

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“…A hand-held mass spectrometry desorption probe using picosecond infrared laser (PIRL) was developed to couple with an optical surgical tracking system ( Fig. 1 f) [ 36 ]. This direct sampling MS system enabled in situ tissue pathology and the histopathological analysis of neuronal tissues in a mouse model showed comparable results between PIRL and the surgical scalpel method ( Fig.…”

Section: Ms Analysis Using Ambient Ionization: Transferring Ms From Laboratories To Clinicsmentioning

confidence: 99%

Recent advances in on-site mass spectrometry analysis for clinical applications

Zhou¹,

Zhang²,

Ouyang³

2022

TrAC Trends in Analytical Chemistry

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In recent years, mass spectrometry (MS) is increasingly attracting interests for clinical applications, which also calls for technical innovations to make a transfer of MS from conventional analytical laboratories to clinics. The system design and analysis procedure should be friendly for novice users and appliable for on-site clinical diagnosis. In addition, the analysis result should be auto-interpreted and reported in formats much simpler than mass spectra. This motivates new ideas for developments in all the aspects of MS. In this review, we report recent advances of direct sampling ionization and miniature MS system, which have been developed targeting clinical and even point-of-care analysis. We also discuss the trend of the development and provide perspective on the technical challenges raised by diseases such as coronavirus SARS-CoV-2.

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In situ tissue pathology from spatially encoded mass spectrometry classifiers visualized in real time through augmented reality

Abstract: Integration between a hand-held mass spectrometry desorption probe based on picosecond infrared laser technology (PIRL-MS) and an optical surgical tracking system demonstrates in situ tissue pathology from point-sampled mass spectrometry...

Cited by 25 publications

References 47 publications

Robot-Assisted SpiderMass for In Vivo Real-Time Topography Mass Spectrometry Imaging

Robot-Assisted SpiderMass for In Vivo Real-Time Topography Mass Spectrometry Imaging

Cancer Surgery 2.0: Guidance by Real-Time Molecular Technologies

Recent advances in on-site mass spectrometry analysis for clinical applications

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