<i>In vitro</i>ageing of pig oocytes: effects of the histone deacetylase inhibitor trichostatin A

Ješeta, Michal; Petr, Jaroslav; Krejcova, Tereza; Eva, Carola; Jílek, F.

doi:10.1017/s0967199408004668

Cited by 27 publications

(15 citation statements)

References 54 publications

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“…To delay or even prevent the ageing process in oocytes stored in vitro, treating them with some chemicals and antioxidant agents, such as caffeine in pigs (Kikuchi et al 2002), nitric oxide (Goud et al, 2005 a, b) and DL-dithiothreitol in mice (Rausell et al, 2007) and humans (Tarin et al, 1998) and trichostatine A in pig (Jeseta et al, 2008) and mice (Huang et al, 2007) has been proposed and applied. In addition, melatonin has been reported to reduce oxidative stress in aged mouse oocytes and to delay the onset of apoptosis, which is the end point of the oocyte ageing process .…”

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confidence: 99%

Fish Oocyte Ageing and its Effect on Egg Quality

Samarin

Policar

Lahnsteiner

2015

Reviews in Fisheries Science & Aquaculture

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In fish, delayed spawning in nature, delayed egg collection in capture and delayed fertilization after egg stripping can lead to oocyte ageing and over-ripening. Oocyte ageing is reported as one of the most important factors affecting the egg quality. During over-ripening, morphological, physiological, biochemical, histological, cellular, and molecular changes occur inside the eggs and ovarian fluid. These alterations can negatively affect the egg fertilization capacity and successive developmental stages. The time period during which eggs remain viable after ovulation or stripping has been reported from a few minutes to a few weeks depending on the fish species and the storage temperature. Although several biomarkers that characterize the over-ripening phenomenon have been defined, the reliability of these parameters in the field of aquaculture is controversial. For future researches, inhibition of oocyte ageing by antioxidants could be a valuable research topic. In the present article, the morphological, physiological, biochemical, histological, cellular, and molecular changes that occur during oocyte over-ripening under in vivo conditions and during in vitro storage are reviewed and possible reasons for the decrease in viability of eggs are presented. Species-specific changes in the egg viability and larval development are also considered.

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confidence: 99%

Fish Oocyte Ageing and its Effect on Egg Quality

Samarin

Policar

Lahnsteiner

2015

Reviews in Fisheries Science & Aquaculture

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“…In addition, it was recently shown that it inhibits histone deacetylase (HDAC; Marks and Breslow 2007). Moreover, Jeseta et al (2008) showed that HDAC inhibition delays spontaneous aging in pig oocytes. In the present study, mouse oocytes were treated with HDAC inhibitors such as trichostatin A and sodium butyrate to determine whether the effects of DMSO are linked to HDAC inhibition.…”

Section: Discussionmentioning

confidence: 99%

Dimethyl sulfoxide inhibits spontaneous oocyte fragmentation and delays inactivation of maturation promoting factor (MPF) during the prolonged culture of ovulated murine oocytes in vitro

Choi

2011

Cytotechnology

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In this study, the effects of dimethyl sulfoxide (DMSO) on the spontaneous aging of ovulated murine oocyte were evaluated in vitro. When ovulated oocytes were cultured continuously in vitro without fertilization stimulation, they underwent several phenotypic changes, including non-activation, activation, fragmentation, and lysis. To investigate the effects of DMSO on these changes, I cultured ovulated oocytes with various concentrations of DMSO and evaluated the phenotypic changes for up to 3 days. After 3 days of culture, the frequency of oocyte fragmentation was significantly lower in oocytes treated with 2 and 4% DMSO (7 and 5%, respectively) than in control oocytes (80%). All control oocytes were activated or fragmented after 3 days of culture in vitro. However, more than 80% of the oocytes cultured with 4% DMSO for 3 days contained spindles and condensed chromosomes, although they displayed abnormal spindle structures. Next Cdk1 activity in DMSO-treated oocytes was examined. The results showed that DMSO treatment prevented the reduction in Cdk1 activity during prolonged culture. Moreover, DMSO inhibited the degradation of cyclin B. These results suggest that DMSO inhibits spontaneous oocyte fragmentation and maintains Cdk1 activity in ovulated murine oocytes during prolonged culture in vitro, possibly by inhibiting cyclin B degradation.

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“…Akiyama et al (2006) demonstrated that manipulation of histone acetylation in maturing oocytes induces aneuploidy and has a detrimental effect on subsequent embryonic development. On the other hand, TSA is able to enhance the viability of oocytes matured to metaphase II that were not fertilized and underwent a spontaneous detrimental process designated as aging (Jeseta et al, 2008). Also, Rybouchkin et al (2006) described the positive effects of histone deacetylase inhibition on the development of embryos that had been created using somatic cell nuclear transfer.…”

Section: Discussionmentioning

confidence: 99%

Histone deacetylase inhibition improves meiotic competence but not developmental competence in growing pig oocytes

Petr

Eva

Kheilová

et al. 2009

Zygote

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SummaryIn fully grown pig oocytes, meiotic maturation in vitro is retarded by inhibition of histone deacetylases by trichostatin A (TSA). In growing oocytes with partial meiotic competence, culture with TSA has no significant effect on the meiotic maturation. Growing oocytes treated with TSA mature mainly to metaphase I. The ratio of oocytes that mature to metaphase II is very limited. After transient exposure to TSA, the maturation of growing oocytes with partial meiotic competence takes a different course. When these oocytes are first cultured in a TSA-free medium, then cultured for another 24 h with 100 nM TSA and finally again in a TSA-free medium for 24 h, the ratio of oocytes that mature to metaphase II significantly increases reaching 59%. When oocytes were cultured for the same length of time without transient exposure to TSA, only 19% matured to metaphase II. Those oocytes that matured to metaphase II after transient exposure to TSA were successfully activated using calcium ionophore. However, the subsequent cleavage was very limited. We can conclude that transient exposure of growing pig oocytes with partial meiotic competence to TSA increases oocyte meiotic competence, but it does not enhance developmental competence after parthenogenetic activation.

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In vitroageing of pig oocytes: effects of the histone deacetylase inhibitor trichostatin A

Cited by 27 publications

References 54 publications

Fish Oocyte Ageing and its Effect on Egg Quality

Fish Oocyte Ageing and its Effect on Egg Quality

Dimethyl sulfoxide inhibits spontaneous oocyte fragmentation and delays inactivation of maturation promoting factor (MPF) during the prolonged culture of ovulated murine oocytes in vitro

Histone deacetylase inhibition improves meiotic competence but not developmental competence in growing pig oocytes

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