<i>In Vitro</i>and<i>Ex Vivo</i>Effects of Recent and New Macrolide Antibiotics on Chemotaxis of Human Polymorphonuclear Leukocytes

Torre, Donato; Broggini, M.; Botta, V.; Sampietro, Carmen; Busarello, R.; Garberi, C.

doi:10.1080/1120009x.1991.11739097

Cited by 26 publications

(8 citation statements)

References 9 publications

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“…Less is understood about the mechanism of the clinically discrepant effects between 14‐ and 16‐membered ring macrolides in long‐term low‐dose therapy 9 . A number of researchers examined the potency of several kinds of macrolides against neutrophil activity such as motility, phagocytosis, and oxygen radical generation, and did not find any significant differences between josamycin and 14‐membered ring macrolides 9,46‐50 . This suggests that the effectiveness of long‐term low‐dose macrolide therapy does not depend on the direct effects on neutrophil functions.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Macrolides on Interleukin‐8 Secretion From Cultured Human Nasal Epithelial Cells

et al. 1997

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The mechanism of macrolide therapy in chronic sinusitis patients is unclear. The authors studied the effect of macrolides on interleukin (IL)-8 secretion from cultured human nasal epithelial cells. Epithelial cells harvested from the nasal polyps of patients with chronic sinusitis were primary-cultured, and secreted IL-8 in culture media was measured by enzyme immunoassay. The cells secreted considerable amounts of IL-8 constitutively and in response to lipopolysaccharide. The secretion was significantly inhibited by 10(-5) M of erythromycin, clarithromycin, roxithromycin, and josamycin. 10(-6) M erythromycin still showed the inhibitory effect, whereas the same concentration of josamycin did not. These results indicate that macrolide antibiotics may act as an immunomodulator to reduce IL-8 in inflammatory sites and, at least partially, account for the clinically discrepant effects between 14- and 16-membered ring macrolides in long-term low-dose therapy for chronic sinusitis.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Macrolides on Interleukin‐8 Secretion From Cultured Human Nasal Epithelial Cells

et al. 1997

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“…Further evidence comes from Torre et al, who studied ex vivo neutrophil chemotaxis in 5 normal adults following ingestion of therapeutic doses of erythromycin, josamycin, miokamycin, and roxithromycin for 4 days. 31 They demonstrated a reduction in neutrophil chemotaxis to chemoattractant casein following isolation of neutrophils from the volunteers. This was con®rmed in vitro only at greater-than-therapeutic concentrations, which, the authors conclude, may have re¯ected a toxic effect of the macrolides on the neutrophils.…”

Section: Chemotaxismentioning

confidence: 99%

Anti‐inflammatory effects of macrolides in lung disease*

Jaffé¹,

Bush²

2001

Pediatric Pulmonology

188

106

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During the past five decades there has been increasing interest in the potential anti-inflammatory effects of macrolide antibiotics. Low-dose macrolide therapy has dramatically increased survival in patients with diffuse panbronchiolitis, a disease with many similarities to cystic fibrosis (CF). This has led to further investigation into the potential use of macrolides in chronic lung diseases with an inflammatory component. This review summarizes the proposed anti-inflammatory mechanisms for this group of antibiotics, and examines the effect of macrolides on modulation of the inflammatory pathways, neutrophil function, bronchoconstriction, Pseudomonas biofilm, mucus rheology, bacterial adherence, and multidrug-resistant protein. It discusses the current status of clinical studies in children with CF, bronchiectasis, and asthma. While there are much in vitro data for different proposed anti-inflammatory effects, randomized controlled clinical trials in children with CF, bronchiectasis, and asthma are still just beginning. The benefits and potential side effects need to be determined before routine use can be advised.

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“…The concentration of macrolide needed in vitro to inhibit chemotaxis of peripheral blood neutrophils from healthy volunteers was much greater than therapeutic doses (293). Anderson (6) demonstrated that erythromycin increased neutrophil migration in response to a leukocyte attractant in vitro.…”

Section: Effects On Inflammationmentioning

confidence: 99%

Mechanisms of Action and Clinical Application of Macrolides as Immunomodulatory Medications

2010

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SUMMARY Macrolides have diverse biological activities and an ability to modulate inflammation and immunity in eukaryotes without affecting homeostatic immunity. These properties have led to their long-term use in treating neutrophil-dominated inflammation in diffuse panbronchiolitis, bronchiectasis, rhinosinusitis, and cystic fibrosis. These immunomodulatory activities appear to be polymodal, but evidence suggests that many of these effects are due to inhibition of extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation and nuclear factor kappa B (NF-κB) activation. Macrolides accumulate within cells, suggesting that they may associate with receptors or carriers responsible for the regulation of cell cycle and immunity. A concern is that long-term use of macrolides increases the emergence of antimicrobial resistance. Nonantimicrobial macrolides are now in development as potential immunomodulatory therapies.

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In VitroandEx VivoEffects of Recent and New Macrolide Antibiotics on Chemotaxis of Human Polymorphonuclear Leukocytes

Cited by 26 publications

References 9 publications

Inhibitory Effect of Macrolides on Interleukin‐8 Secretion From Cultured Human Nasal Epithelial Cells

Inhibitory Effect of Macrolides on Interleukin‐8 Secretion From Cultured Human Nasal Epithelial Cells

Anti‐inflammatory effects of macrolides in lung disease*

Mechanisms of Action and Clinical Application of Macrolides as Immunomodulatory Medications

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