In vitro and in vivo studies of Syzygium cumini-loaded electrospun PLGA/PMMA/collagen nanofibers for accelerating topical wound healing

Abdelazim, Esraa B.; Abed, Tasneem; Goher, Shaimaa S.; Alya, Shaza H.; El-Nashar, Heba A. S.; EL-Moslamy, Shahira H.; El-Fakharany, Esmail M.; Abdul-Baki, Enas A.; Shakweer, Marwa Mosaad; Eissa, Noura G.; Elsabahy, Mahmoud; Kamoun, Elbadawy A.

doi:10.1039/d3ra06355k

Cited by 13 publications

(2 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the natural products, plants are common sources of traditional healing agents worldwide, especially in the poor and less developed countries ( Ashmawy et al, 2023 ; Mia et al, 2023 ). On the other hand, people in developed countries are now conscious of the adverse events of modern drugs, thus there is a developing enthusiasm for research into and utilization of plant-mediated products or preparations ( Abdelazim et al, 2024 ; Shill et al, 2024 ). Plant extracts contain numerous groups and components, including alkaloids, glycosides, phenolics and flavonoids, vitamins, and minerals ( Gunathilake et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Phytochemical analysis and biological investigation of Cheilanthes tenuifolia (Burm.f.) Swartz

Juhi,

El-Nashar,

Al Faruq

et al. 2024

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

Introduction:Cheilanthes tenuifolia is an evergreen ornamental small fern, belonging to the family Pteridaceae, that grows in warm and rocky regions worldwide. Many species of Cheilanthes genus are evidently endowed with important phytochemicals and bioactivities. This study aimed to perform a preliminary phytochemical analysis of Cheilanthes tenuifolia leaves alongside an evaluation of free radical scavenging, anti-inflammatory, antimicrobial, and clot lysis activities of extract fractions.Materials and methods: A preliminary phytochemical analysis was done after fractionation of ethanolic extract (ECT) with n-hexane (HCT) and chloroform (CCT). Then, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, egg albumin and RBC membrane stabilization tests, disc diffusion, and human blood clot lysis assays were performed.Results: Phytochemical investigations suggested that the plant is rich in alkaloids, glycosides, tannins, and flavonoids. All obtained fractions exhibited concentration-dependent radical scavenging, inhibition of egg protein denaturation and RBC membrane lysis capacities. Except for antifungal tests, ECT exhibited better DPPH radical scavenging, anti-inflammatory, antibacterial, and clot lysis capacities than HCT and CCT fractions. However, all fractions exhibited a mild anti-inflammatory activity.Conclusion:C. tenuifolia might be a good source of antioxidant, anti-microbial, and anti-atherothrombotic agents. Further studies are required to isolate and characterize the active principles liable for each bioactivity, along with possible molecular interactions.

show abstract

Section: Discussionmentioning

confidence: 99%

Phytochemical analysis and biological investigation of Cheilanthes tenuifolia (Burm.f.) Swartz

Juhi,

El-Nashar,

Al Faruq

et al. 2024

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Natural products and their derivatives are promising sources of anti-inflammatory agents ( Hou et al, 2020 ; Ashmawy et al, 2023 ; Abdelazim et al, 2024 ). It may be due to their promising anti-microbial properties as well as the inhibitory or neutralizing capacity of pro-inflammatory and inflammatory mediators ( Azab et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory effects of thymol: an emphasis on the molecular interactions through in vivo approach and molecular dynamic simulations

Islam,

Bappi,

Bhuia

et al. 2024

Front. Chem.

Self Cite

View full text Add to dashboard Cite

Thymol (THY), as the natural monoterpene phenol, acts against oxidative stress and inflammatory processes. This study aimed to evaluate the anti-inflammatory effects and possible molecular mechanisms of THY via formalin-induced mouse and egg albumin-induced chick models alongside molecular docking and molecular dynamic (MD) simulations. THY (7.5, 15, and 30 mg/kg) was investigated, compared to celecoxib and ketoprofen (42 mg/kg), as anti-inflammatory standards. THY dose-dependently and significantly (p < 0.05) decreased paw-licking and edema diameter parameters in formalin (phases I and II) and egg albumin-induced models. Moreover, THY (15 mg/kg) exerted better anti-inflammatory effects in combination with the standard drug ketoprofen than alone and with celecoxib. In silico studies demonstrated elevated binding affinities of THY with cyclooxygenase-2 (COX-2) than the COX-1 enzyme, and the ligand binds at a similar location where ketoprofen and celecoxib interact. The results of MD simulations confirmed the stability of the test ligand. THY exerted anti-inflammatory effects on Swiss mice and young chicks, possibly by interacting with COX-2. As a conclusion, THY might be a hopeful drug candidate for the management of inflammatory disorders.

show abstract

Small Steps to the Big Picture for Health‐Promoting Applications Through the Use of Chickweed (Stellaria media): In Vitro, In Silico, and Pharmacological Network Approaches

Cusumano,

Flores,

Cetiz

et al. 2024

Food Science & Nutrition

View full text Add to dashboard Cite

Stellaria media L., also called chickweed, is widespread in all parts of the world. In the present study, we investigated the biological properties and chemical profiles of different extracts (ethyl acetate, ethanol, ethanol/water, and water) of S. media. The chemical profiles were examined using UHPLC/MS/MS technique. Regarding the biological properties, antioxidant properties as well as enzyme‐inhibiting and cytotoxic effects of the extracts were demonstrated by in vitro methods. To obtain further information about the structure‐ability relationship, network pharmacology and molecular docking were also performed. Twelve phenolic compounds were identified in the extracts and most of them were flavonoids (apigenin, kaempferol derivatives, etc.). The water extract showed the best free radical scavenging activity, while the ethanol was the most active in reducing power tests. When inhibiting AChE, the ethyl acetate extract showed the best inhibitory effect. The water extract has a good cytotoxic effect on HepG2 (cell viability: 33.9% at a concentration of 100 g/mL). The analysis, performed using the STRING database, included these 45 cancer‐associated targets. The identified hub genes were TP53, CDKN2A, PTEN, KRAS, and HRAS. In molecular docking analysis, acacetin‐O‐hexoside‐O‐deoxyhexoside and napigenin‐7‐O‐hexoside exhibit remarkable binding energies with proteins. Consequently, S. media can be potential raw materials for designing functional formulations in the pharmaceutical, nutraceutical, and cosmeceutical industries.

show abstract

In vitro and in vivo studies of Syzygium cumini-loaded electrospun PLGA/PMMA/collagen nanofibers for accelerating topical wound healing

Abstract: Fabrication of Syzygium cumini-loaded polymeric nanofibers for wound healing applications.

Cited by 13 publications

References 52 publications

Phytochemical analysis and biological investigation of Cheilanthes tenuifolia (Burm.f.) Swartz

Phytochemical analysis and biological investigation of Cheilanthes tenuifolia (Burm.f.) Swartz

Anti-inflammatory effects of thymol: an emphasis on the molecular interactions through in vivo approach and molecular dynamic simulations

Small Steps to the Big Picture for Health‐Promoting Applications Through the Use of Chickweed (Stellaria media): In Vitro, In Silico, and Pharmacological Network Approaches

Contact Info

Product

Resources

About