In vitro and in vivo antibiofilm activity of a coral associated actinomycete against drug resistant Staphylococcus aureus biofilms

Bakkiyaraj, Dhamodharan; Pandian, Shunmugiah Karutha

doi:10.1080/08927014.2010.511200

Cited by 118 publications

(63 citation statements)

References 33 publications

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“…Apart from antimicrobials, coral associated bacteria including actinomycetes have been reported to produce quorum sensing inhibitors and antibiofilm compounds against the notorious human pathogen Streptococcus pyogenes (Thenmozhi et al 2009;Nithyanand et al 2010). As the incidence of drug resistant bacteria and its virulence mechanisms are on the rise, coral associated bacteria might be targeted for novel pharmaceutical and anti virulent compounds as it has recently been shown that coral associated actinomycetes efficiently inhibited the biofilms of methicillin resistant Staphylococcus aureus (MRSA) both in vitro and in vivo using the nematode Caenorhabditis elegans (Bakkiyaraj and Pandian 2010). Apart from this, coral associated microbial communities play a symbiotic role by defending the coral holobiont against pathogens and assimilating nutrients from the surrounding ecosystem to the coral holobiont.…”

Section: Discussionmentioning

confidence: 99%

Culture independent characterization of bacteria associated with the mucus of the coral Acropora digitifera from the Gulf of Mannar

Nithyanand

Indhumathi

Ravi

et al. 2010

World J Microbiol Biotechnol

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Corals are sessile eukaryotic hosts which provide a unique surface for microbial colonization. Culture independent studies show that the coral mucus and tissue harbour diverse and abundant prokaryotic communities. However, little is known about the diversity of bacteria associated with the corals of Gulf of Mannar. The present study characterised the bacterial diversity associated with the mucus of the coral Acropora digitifera from the Gulf of Mannar by 16S rRNA gene clone library construction. The bacterial communities of the mucus of A. digitifera were diverse, with representatives within the Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes and several unclassified bacteria. The culture independent bacterial population was totally different from our previous culture dependent study of the mucus and tissue of the same coral. 36% of the bacteria in the clone library of A. digitifera were found to be novel after full length sequencing of the 16S rRNA gene wherein several clones were found to be novel at the Genus and species level. The current study further supports the findings that Actinobacteria amount to a certain proportion among bacterial communities associated with corals.

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Section: Discussionmentioning

confidence: 99%

Culture independent characterization of bacteria associated with the mucus of the coral Acropora digitifera from the Gulf of Mannar

Nithyanand

Indhumathi

Ravi

et al. 2010

World J Microbiol Biotechnol

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“…As examples, an extract from Streptomyces albus inhibited biofilm formation of Vibrio harveyi (You et al 2007), and methanol extracts of coral-associated S. akiyoshiensis inhibited biofilm formation of S. aureus (Bakkiyaraj and Pandian 2010). However, no further information on these anti-biofilm compounds and their mechanisms of biofilm reduction has been reported.…”

Section: Introductionmentioning

confidence: 97%

Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation

et al. 2011

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Bacterial biofilms are associated with chronic infections due to their resistance to antimicrobial agents. Staphylococcus aureus is a versatile human pathogen and can form biofilms on human tissues and diverse medical devices. To identify novel biofilm inhibitors of S. aureus, the supernatants from a library of 458 Actinomycetes strains were screened. The culture supernatants (1% v/v) of more than 10 Actinomycetes strains inhibited S. aureus biofilm formation by more than 80% without affecting the growth. The culture supernatants of these biofilm-reducing Actinomycetes strains contained a protease (equivalent to 0.1 μg proteinase K ml(-1)), which both inhibited S. aureus biofilm formation and detached pre-existing S. aureus biofilms. This study suggests that protease treatment could be a feasible tool to reduce and eradicate S. aureus biofilms.

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“…Biofilm inhibitory concentration (BIC)-lowest concentration required to inhibit the biofilm formation-of the extracts was assessed against P. aeruginosa PAO1 with varying concentrations (5-2,500 lg mL -1 ) and found to be 1.75 mg mL -1 . Further, the activity of CAB extracts on the growth of P. aeruginosa PAO1 was studied and found no significant reduction in growth of CAB treated cultures which further prove the potential of CAB extracts as a candidate for QSI.Inhibition of biofilm formation by the CAB extracts was studied as earlier [11]. Both the light microscopic and confocal images have shown the reduction in surface area covered by the biofilms in slides treated with extracts (Fig.…”

mentioning

confidence: 99%

“…Inhibition of biofilm formation by the CAB extracts was studied as earlier [11]. Both the light microscopic and confocal images have shown the reduction in surface area covered by the biofilms in slides treated with extracts (Fig.…”

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confidence: 99%

Anti-pathogenic Potential of Coral Associated Bacteria Isolated from Gulf of Mannar Against Pseudomonas aeruginosa

2012

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Infections of Pseudomonas aeruginosa are of great concern because of its increasing resistance towards conventional antibiotics. Quorum sensing system of P. aeruginosa acts as a global regulator of almost all the virulence factors and majorly its biofilm formation. In the present study, quenching of QS system of P. aeruginosa has been explained with bioactives from bacteria associated with the coral Acropora digitifera. Isolated bioactives inhibited the expression of various virulence traits of P. aeruginosa like biofilm formation, and the production of extracellular enzymes like protease and elastase. This study also emphasises the potential of coral associated bacteria in producing bioactive agents with anti-pathogenic properties.Keywords Quorum sensing Á Pseudomonas aeruginosa Á Coral associated bacteria Á Biofilm Á Anti-QS Á Quorum sensing inhibitor Density dependent cell to cell communication between bacterial population known as quorum sensing (QS) is of great interest, since virulence and resistance being its major outcome in bacteria. It has been reported that QS controls the regulation of virulence and biofilm formation that are 1000-fold more resistant to antibiotics [1].Pseudomonas aeruginosa, an opportunistic pathogen causes infections in cystic fibrosis (CF), burns, wounds and pneumonia patients [1]. QS plays a major role in controlling the virulence of P. aeruginosa by regulating various virulence traits viz. LasA protease, LasB elastase, superoxide dismutase, alkaline protease, hydrogen cyanide, pyocyanin, pyoverdin, rhamnolipid and biofilm formation [1].QS being the major factor concerning enhanced virulence and broad spectrum resistance in P. aeruginosa, its inhibition will be of importance to treat infections caused by pathogenic drug resistant P. aeruginosa. Compounds with the ability to interfere the QS system of bacteria are called as QS inhibitors (QSI) or anti-pathogenic agents [2].In recent past, many sources were explored for QSI, and few with the potential to inhibit various pathogens were reported from plants, synthetic chemicals and microbes [3][4][5][6][7]. Because of the increasing rate of rediscovery of known drugs from terrestrial sources, marine microorganisms have gained much attention, since many remain unexplored [8]. In this study, coral associated bacteria (CAB) were screened for antibiofilm and anti-QS properties, since they play a defensive role in coral reefs in controlling the pathogens of the host [9].Out of 41 CAB screened, nine have shown anti-QS activity against the bioreporter strain Chromobacterium violaceum ATCC 12472 (Fig. 1a) [10]. CAB23 and 41 which showed around 90 % anti-QS activity against C. violaceum were studied further. Biofilm inhibitory concentration (BIC)-lowest concentration required to inhibit the biofilm formation-of the extracts was assessed against P. aeruginosa PAO1 with varying concentrations (5-2,500 lg mL -1 ) and found to be 1.75 mg mL -1 . Further, the activity of CAB extracts on the growth of P. aeruginosa PAO1 was studied and fou...

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In vitro and in vivo antibiofilm activity of a coral associated actinomycete against drug resistant Staphylococcus aureus biofilms

Cited by 118 publications

References 33 publications

Culture independent characterization of bacteria associated with the mucus of the coral Acropora digitifera from the Gulf of Mannar

Culture independent characterization of bacteria associated with the mucus of the coral Acropora digitifera from the Gulf of Mannar

Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation

Anti-pathogenic Potential of Coral Associated Bacteria Isolated from Gulf of Mannar Against Pseudomonas aeruginosa

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