In vitro and in vivo characterization of the anticancer activity of Thai stingless bee (Tetragonula laeviceps) cerumen

Nugitrangson, Pongvit; Puthong, Songchan; Iempridee, Tawin; Pimtong, Wittaya; Pornpakakul, Surachai; Chanchao, Chanpen

doi:10.1177/1535370215600102

Cited by 25 publications

(24 citation statements)

References 35 publications

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“…33 Briefly, CIM-16 plates comprise an upper and a lower chamber separated by a microporous membrane (8 mm pores) with microelectrodes at the underside for impedance-based detection of migrated cells. Initially, 175 mL complete medium and 50 mL serum-free medium were added to the lower and upper chambers of the CIM-16 plate, respectively.…”

Section: Annexin V Stainingmentioning

confidence: 99%

Long non-coding RNA H19 enhances cell proliferation and anchorage-independent growth of cervical cancer cell lines

Iempridee

2016

Exp Biol Med (Maywood)

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Long non-coding RNA H19 is aberrantly expressed in multiple malignancies and its expression levels correlate with recurrence, metastasis, and patient survival. Despite numerous reports documenting the role of H19 in carcinogenesis, its contribution to cervical cancer development is still largely unknown. In this study, I observed that H19 expression was elevated in cervical cancer cell lines and could be detected in extracellular vesicles in the culture medium. In addition, I demonstrated, by overexpression and knockdown experiments, that H19 promoted cell proliferation and multicellular tumor spheroid formation without significantly affecting apoptosis and cell migration. Finally, treatment with transforming growth factor beta and hypoxia-mimetic CoCl 2 could modulate H19 levels in a cell line-specific manner. These findings indicate that H19 promotes both anchorage-specific andindependent growth of cervical cancer cell lines and may serve as a potential target for cancer diagnosis and therapy.

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Section: Annexin V Stainingmentioning

confidence: 99%

Long non-coding RNA H19 enhances cell proliferation and anchorage-independent growth of cervical cancer cell lines

Iempridee

2016

Exp Biol Med (Maywood)

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“…Other advantages include transparent embryos, which permit clear observations in the early stages of development, high fecundity, similar development to that of mammals, and their genetic similarities to humans. 12 In our previous work, we reported the effects of a-mangostin on the embryonic angiogenesis of zebrafish and did not find anti-angiogenic properties of a-mangostin in zebrafish embryos, despite the clear downregulation of the expression of vegfaa and vegfr2 transcripts; 21 however, we did not study other effects on zebrafish embryos than those on angiogenesis. In the present study, we therefore investigated the toxic effects of a-mangostin on embryonic development.…”

Section: Discussionmentioning

confidence: 73%

Acute toxicity and teratogenicity of α-mangostin in zebrafish embryos

Kittipaspallop

Taepavarapruk

Chanchao

et al. 2018

Exp Biol Med (Maywood)

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α-Mangostin is the most active compound derived from the pericarps of mangosteen. A number of studies have reported its anticancer activity. However, only a few studies have investigated the toxicity of this compound; moreover, the teratogenicity of α-mangostin has not been reported. In this study, we evaluated the effects of α-mangostin on the development of zebrafish embryos. The exposure of zebrafish embryos to α-mangostin for 72 h dose-dependently induced mortality and abnormal development. The derived LC50 value of α-mangostin to zebrafish embryos at 72 h was 5.75 ± 0.26 μmol/L. We observed teratogenic effects in α-mangostin-treated embryos, characterized by axis malformation, bent tail, pericardial edema, yolk sac edema, sluggish circulation, and heart malformation. The percentages of the malformed embryos were 78.79% and 100% at 4.5 and 6 μM, respectively. α-Mangostin exposure also caused hemostasis in the ducts of Cuvier and a decreased heart rate in zebrafish embryos at 48 and 72 h post-fertilization. These results indicated that α-mangostin induced cardiac dysfunction in zebrafish embryos. In addition, a sub-lethal concentration and a lethal concentration (3 and 6 μM, respectively) were used to assess the compound effects on oxidative stress and embryonic erythropoiesis. α-Mangostin was found to decrease the level of reactive oxygen species (ROS) in zebrafish embryos. Furthermore, hemoglobin staining revealed a decrease in hemoglobin, which suggested that α-mangostin disrupted embryonic erythropoiesis in zebrafish. To our knowledge, the results of this study, for the first time, demonstrated that α-mangostin was potentially teratogenic and could disrupt embryonic ROS balance and erythropoiesis. Impact statement α-Mangostin has been reported to have anticancer properties both in vitro and in vivo models. Although there are several studies that evaluated the toxicity of the compound in rodent models, we are the first to evaluate the teratogenicity of α-mangostin. In the present work, we found that α-mangostin induced mortality and malformations in zebrafish embryos. In addition, we exhibited that the compound also disrupted the reactive oxygen species and hemoglobin levels. These findings suggest that α-mangostin may possibly cause the same adverse effects on human health. The mechanisms of these toxicological effects of the compound will be further elucidated and the effects found in zebrafish embryos need to be verified in other animal models.

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“…Stingless bee cerumen extracts have also been used as a potential anticancer agent with human breast, lung, liver, stomach and colon cancer cell lines, inducing high cytotoxicity and an apoptotic like cell morphology [ 15 ]. This study also showed that α-mangostin isolated from cerumen induced in vitro cytotoxicity against the above cell lines and in-vivo cytotoxicity against zebrafish embryos [ 15 ].…”

Section: Stingless Bee Cerumenmentioning

confidence: 99%

“…It is important to note that stingless bee products are not restricted to honey, but can involve several products including propolis, beebread, cerumen and bee pollen ( Figure 2 ). Although these are not as common as honey, studies have reported several medicinal properties in those products too [ 14 , 15 , 16 ]. Based on a comparison between the studies on stingless bees and honeybees, a significant gap in research on stingless bee products has been identified compared to the common honeybee products that are well-studied ( Figure 3 ).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant-Based Medicinal Properties of Stingless Bee Products: Recent Progress and Future Directions

et al. 2020

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Stingless bees are a type of honey producers that commonly live in tropical countries. Their use for honey is being abandoned due to its limited production. However, the recent improvements in stingless bee honey production, particularly in South East Asia, have brought stingless bee products back into the picture. Although there are many stingless bee species that produce a wide spread of products, known since old eras in traditional medicine, the modern medical community is still missing more investigational studies on stingless bee products. Whereas comprehensive studies in the current era attest to the biological and medicinal properties of honeybee (Apis mellifera) products, the properties of stingless bee products are less known. This review highlights for the first time the medicinal benefits of stingless bee products (honey, propolis, pollen and cerumen), recent investigations and promising future directions. This review emphasizes the potential antioxidant properties of these products that in turn play a vital role in preventing and treating diseases associated with oxidative stress, microbial infections and inflammatory disorders. Summarizing all these data and insights in one manuscript may increase the commercial value of stingless bee products as a food ingredient. This review will also highlight the utility of stingless bee products in the context of medicinal and therapeutic properties, some of which are yet to be discovered.

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In vitro and in vivo characterization of the anticancer activity of Thai stingless bee (Tetragonula laeviceps) cerumen

Cited by 25 publications

References 35 publications

Long non-coding RNA H19 enhances cell proliferation and anchorage-independent growth of cervical cancer cell lines

Long non-coding RNA H19 enhances cell proliferation and anchorage-independent growth of cervical cancer cell lines

Acute toxicity and teratogenicity of α-mangostin in zebrafish embryos

Antioxidant-Based Medicinal Properties of Stingless Bee Products: Recent Progress and Future Directions

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