<i>In vitro</i>and<i>in vivo</i>effects of clove on pro-inflammatory cytokines production by macrophages

Rodrigues, T. G. B.; Fernandes, Ana Angélica Henrique; Sousa, João Paulo Barreto de; Bastos, Jairo Kenupp; Sforcin, José Maurício

doi:10.1080/14786410802242679

Cited by 67 publications

(43 citation statements)

References 25 publications

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“…In vivo, treatment with clove/eugenol reduced the ability of mice exposed to thioacetamide (and subsequently undergoing hepatic damage) to produce/release TNF into their systemic circulation (Yogalakshmi et al, 2010). Based on in vitro and in vivo studies of the effects of clove on pro-inflammatory cytokine production by macrophages, Rodrigues et al (2009) claimed eugenol was the causative agent for the particular changes in cytokine formation by cells cultured for 24 h. Those results, like those in the present study, built on evidence from Lee et al (2007) showing that eugenol suppressed production of LPS-induced TNF formation by phagocytes treated in culture for 20 h.…”

Section: Discussionmentioning

confidence: 99%

“…While some biologic effects from exposure to clove have been reported (and noted above), apart from the above-noted antiinflammatory effects, effects by clove or key constituents (like eugenol) on specific immune system components/mechanisms have only recently begun to be examined in detail (Bachiega et al, 2012;Grespan et al, 2012;Lee et al, 2007;Rodrigues et al, 2009;Yogalakshmi et al, 2010). As many of those studies have often yielded conflicting results, we undertook a series of studies that would hopefully provide some clarity regarding immunotoxicity/immunomodulatory effects of clove-associated materials.…”

Section: Introductionmentioning

confidence: 99%

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Immunomodulatory effects of clove (Syzygium aromaticum) constituents on macrophages:In vitroevaluations of aqueous and ethanolic components

Dibazar

Fateh

Daneshmandi

2014

Journal of Immunotoxicology

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The present work sought to investigate potential suppressive effects on mouse macrophages by in vitro treatment with clove (Syzygium aromaticum) ethanolic extracted essential oil (containing eugenol) or its water-soluble extract. Using doses (ranging from 0.001-1000 mg/ml) of each material freshly prepared in the laboratory, cell survival and production of nitric oxide (NO), tumor necrosis factor (TNF)-, interleukin (IL)-6, and IL-12 by the treated cells (that in all cases also had received LPS stimulation) were measured. Results indicated that, except at doses !100 mg/ml, viability was unaffected in all groups. NO release by LPS-stimulated macrophages was generally significantly suppressed by either material; in contrast, low (i.e. 0.001-1 mg/ml) doses of either extract class appeared to enhance NO release by non-LPS (unstimulated)-treated macrophages. Among LPS-stimulated cells, TNF release was also significantly affected by each extract; the ethanolic extract was suppressive at all doses tested, while the aqueous material was so up to 1 mg/ml and then became stimulatory. In contrast, nearly every dose of either extract appeared to stimulate IL-6 release from the LPS-treated cells. Effects on IL-12 production were overall inconsistent; in general, the ethanolic extract tended to be stimulatory of production by the LPS-treated cells. The data for the aqueous material showed no discernable pattern of effect. The results suggest that clove extracts do not have a distinct cytotoxic activity, but do impart potential anti-and pro-oxidant effects in cells, depending on their concentrations and on the activation state of the macrophages themselves at the time of exposure to the extracts. The impact of the extracts on macrophage cytokine release also displays a pattern of dose-relatedness.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunomodulatory effects of clove (Syzygium aromaticum) constituents on macrophages:In vitroevaluations of aqueous and ethanolic components

Dibazar

Fateh

Daneshmandi

2014

Journal of Immunotoxicology

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“…It is possible that macrophages from various species respond differently to eugenol. Thus, while in our hands unstimulated human mononuclears did not respond to eugenol as for cytokine production, Rodrigues et al [18] have reported that eugenol inhibited the secretion of IL-1β and IL-6 by mouse macrophages. Apparently, Th1 and Th2 cells react adversely to eugenol -while Th1 cytokines TNF-α and IL-2 production by nicotine-induced murine macrophages were down-regulated by eugenol, the generation of Th-2 cytokines IL-10 and TGF-β was activated [19].…”

Section: Discussionmentioning

confidence: 77%

Eugenol as a modulator of the immune dialogue between human mononuclears and colon cancer cells

Djaldetti¹,

Bessler²

2017

J Mol Pathophysiol

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Objective: Cloves (Syzygium aromaticum), the dried flower buds of a tree belonging to the Myrtaceae family have achieved a top place in herbal medicine. To its main active compound eugenol have been attributed definite anti-anaphylactic, anti-inflammatory, and even anticancer properties. It was the object of the study to elucidate the immunomodulatory potential of eugenol and its ability to alter the crosstalk between human peripheral blood mononuclear cells (PBMC) and cells from two human colon carcinoma lines. Materials and Methods: Eugenol, at concentrations of 3.75, 7.5, and 15 µg/ml, was added for 24 h to PBMC alone or to cells co-incubated with either HT-29 or RKO colon carcinoma cells. Subsequently, the generation of the following cytokines was examined: Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IFN-γ, IL-1ra, and IL-10. Results: Eugenol did not affect the capacity of unstimulated PBMC or that of cancer cells to produce any of the cytokines examined. However, when eugenol was added to PBMC co-incubated with HT-29 cells, the production of all cytokines was inhibited, except for IL-6 and IFNγ. The capacity for cytokine generation by PBMC co-incubated with RKO cells was reduced concerning TNF-α and IL-1β. Conclusions: The results indicate that eugenol exerts an immunomodulatory effect on PBMC to produce inflammatory cytokines when stimulated by colon carcinoma cells from the two lines examined. This effect has been shown to be cancer cell type dependent. The capacity of eugenol to interfere with the crosstalk between immune and colon cancer cells may further explain the restraining effect of this compound on cancer development.

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“…[ [96][97][98][99] Eugenol Inhibits IL-1β, TNF and IL-6 release in macrophages. [100,101] Gallic acid Inhibits TNF and IL-6 in HMC-1 cells (10 μM). [102] Gingerol Inhibits IL-1β and IL-12 release in peritoneal macrophages 100 ng/mL) [103] Hydroxytyrosol Inhibits LPS-induced TNF production in THP-1 cells (25, 50 & 100 μM).…”

Section: Lipid Mediator Blockersmentioning

confidence: 99%

Asthma in the 21st Century — Unexpected Applications of Ancient Treatments

Pundir¹,

Kulka²

2013

Using Old Solutions to New Problems - Natural Drug Discovery in the 21st Century

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In vitroandin vivoeffects of clove on pro-inflammatory cytokines production by macrophages

Cited by 67 publications

References 25 publications

Immunomodulatory effects of clove (Syzygium aromaticum) constituents on macrophages:In vitroevaluations of aqueous and ethanolic components

Immunomodulatory effects of clove (Syzygium aromaticum) constituents on macrophages:In vitroevaluations of aqueous and ethanolic components

Eugenol as a modulator of the immune dialogue between human mononuclears and colon cancer cells

Asthma in the 21st Century — Unexpected Applications of Ancient Treatments

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