In VitroandIn VivoEvaluation of Polyherbal Formulation against Russell’s Viper and Cobra Venom and Screening of Bioactive Components by Docking Studies

Sakthivel, G; Dey, Amitabha; Nongalleima, Kh.; Chavali, Murthy; Isaac, R. S. Rimal; Singh, Nidhan; Deb, Lokesh

doi:10.1155/2013/781216

Cited by 19 publications

(13 citation statements)

References 32 publications

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“…Antivenin activities of a few medicinal plants such as Andrographis paniculata, Andrographis lineata, A. bracteolata, Clerodendrum viscosum and Mimosa pudica against the crude venom of Indian cobra have been reported using animal models in the literature (Kumar et al, 2010;Sakthivel et al, 2013;Richard et al, 2006;Meenatchisundaram et al, 2009Meenatchisundaram et al, , 2010. However, it is not a straightforward task to carryout a comparative analysis on the outcomes of the works as the studies are differing from each other in their treatments (pre-treatment/ post-treatment/pre-incubation/etc.…”

Section: Resultsmentioning

confidence: 99%

In vivo studies on detoxifying actions of aqueous bark extract of Prosopis cineraria against crude venom from Indian cobra (Naja naja)

Sivaraman

Rajesh

Elango

2013

Bangladesh J Pharmacol

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Detoxification effect of aqueous, methanol and petroleum ether extracts of medicinal plants such as Aristolochia bracteolata, Mucuna pruriens, Prosopis cineraria and Rauvolfia tetraphylla was systematically screened against lethality of crude venom of Naja naja using Swiss albino mice as animal models. We have herein demonstrated that aqueous bark extract of P. cineraria has substantial anti-venom potential vis-à-vis other extracts used in the present study. The aqueous extract at the dose of 14 mg/kg b.w. was able to almost completely neutralize the lethal activity of 3LD50 (1.1 mg/kg b.w.) of the cobra venom and the extract did not cause any types of adverse side-effects to the animal models. The investigation justifies not only the veraciousness of the extract used by traditional healers of Asian subcontinent as antidotes to snake venoms and also suggests that the aqueous extract should contain specific inhibitors to most principle toxic components of the crude venom. Article Info

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Section: Resultsmentioning

confidence: 99%

In vivo studies on detoxifying actions of aqueous bark extract of Prosopis cineraria against crude venom from Indian cobra (Naja naja)

Sivaraman

Rajesh

Elango

2013

Bangladesh J Pharmacol

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“…A small pilot study was carried out to assess the potency of the reconstituted lyophilized N. naja venom to test if it was comparable to published reports of other commercially available unfractionated, frozen, or lyophilized N. naja venom at 0.3 mg/kg [ 15 , 17 – 19 ].…”

Section: Methodsmentioning

confidence: 99%

Early Treatment with Intranasal Neostigmine Reduces Mortality in a Mouse Model ofNaja naja(Indian Cobra) Envenomation

Lewin

Samuel

Wexler

et al. 2014

Journal of Tropical Medicine

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Objective. Most snakebite deaths occur prior to hospital arrival; yet inexpensive, effective, and easy to administer out-of-hospital treatments do not exist. Acetylcholinesterase inhibitors can be therapeutic in neurotoxic envenomations when administered intravenously, but nasally delivered drugs could facilitate prehospital therapy for these patients. We tested the feasibility of this idea in experimentally envenomed mice. Methods. Mice received intraperitoneal injections of Naja naja venom 2.5 to 10 times the estimated LD50 and then received 5 μL neostigmine (0.5 mg/mL) or 5 μL normal saline by nasal administration. Animals were observed up to 12 hours and survivors were euthanized. Results. 100% of control mice died. Untreated mice injected with 2.5× LD50 Naja naja died at average 193 minutes after injection, while 10 of 15 (67%) of treated mice survived and were behaviorally normal by 6 hours (P < 0.02). In the 5× LD50 group, survival was prolonged from 45 minutes to 196 minutes (P = 0.01) and for 10× LD50 mice, survival increased from 30 to 175 minutes (P < 0.02). Conclusion. This pilot suggests that intranasal drugs can improve survival and is the first direct demonstration that such an approach is plausible, suggesting means by which treatment could be initiated before reaching the hospital. Further investigation of this approach to neurotoxic and other types of envenomation is warranted.

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“…Then the absorbance was measured at 517 nm in Thermo Multiscan Spectrum (Thermo Scientific). Lower absorbance of the reaction mixture indicated higher free radical scavenging activity [20].…”

Section: Antioxidant Activity Dpph Radical Scavenging Assaymentioning

confidence: 99%

“…In the past decades, the therapeutic potential of essential oils and their constituents has been the target of researchers in the pursuit of novel drugs of plant origin, particularly those exhibiting antiinflammatory action, to be used in the prevention or treatment of diseases [20].…”

Section: Introductionmentioning

confidence: 99%

GCMS Based Metabolic Profiling of Essential Oil of Citrus Macroptera Montruz. Leaves and Peel, Assessment of in Vitroantioxidant and Anti-Inflammatory Activity

Nongalleima

Ajungla

Singh

2017

Int J Pharm Pharm Sci

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Objective: The present investigation was designed for Gas Chromatography Mass Spectrometry (GCMS) based metabolite profiling of Citrus macroptera Montruz. Leaves and peel oils followed by assessment of in vitro antioxidant and anti-inflammatory activity. Methods:Essential oil was extracted from leaves and peels of Citrus macroptera Montruz. The oil samples were subjected to GCMS analysis using Shimadzu GCMS-QP2010 equiped with an AOC-2oi auto-injector and AOC-2os autosampler units. In vitro antioxidant activities were evaluated using DPPH radical scavenging, reducing power and nitric oxide reducing method. In vitro anti-inflammatory activity was evaluated using protease inhibitory assay, heat induced haemolysis and albumin denaturation assay. Results:Both the peels and leaves of Citrus macroptera Montruz. Yielded good amount of essential oil. 57 compounds each were identified from leaves as well as peel of C. macroptera. 10 common compounds have been detected in both the oil samples. Peels oil showed IC50 at 118.07 µg/ml and that of leaves showed IC50 at 252.93 µg/ml in DPPH (1, 1-diphenyl-2-picrylhydrazyl) assay. In reducing assay, peel and leaves oil showed IC50 at 122.5 µg/ml and 208.24 µg/ml. In albumin denaturation, the peels showed IC50 at 73.91 µg/ml and that of leaves showed IC50 at 87.48 µg/ml. Conclusion:The oil yield denotes peel as better source of volatile oil than leaves. Essential oil of peel showed more anti-oxidant and antiinflammatory activity than that of leaves essential oil.

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In VitroandIn VivoEvaluation of Polyherbal Formulation against Russell’s Viper and Cobra Venom and Screening of Bioactive Components by Docking Studies

Cited by 19 publications

References 32 publications

<i>In vivo</i> studies on detoxifying actions of aqueous bark extract of <i>Prosopis cineraria</i> against crude venom from Indian cobra (Naja naja)

<i>In vivo</i> studies on detoxifying actions of aqueous bark extract of <i>Prosopis cineraria</i> against crude venom from Indian cobra (Naja naja)

Early Treatment with Intranasal Neostigmine Reduces Mortality in a Mouse Model ofNaja naja(Indian Cobra) Envenomation

GCMS Based Metabolic Profiling of Essential Oil of Citrus Macroptera Montruz. Leaves and Peel, Assessment of in Vitroantioxidant and Anti-Inflammatory Activity

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