In Vitroandin VivoEvaluation of the Efficacy of Bovine Colostrum against Human Rotavirus Infection

Abstract

We found that skimmed and concentrated bovine late colostrum (SCBLC) obtained from normal cows at 6-7 d after parturition exhibited high potency in inhibiting replication of human rotavirus (HRV) in vitro. Furthermore, prophylactic oral administration of SCBLC once before inoculation of HRV prevented the development of diarrhea in suckling mice in vivo. SCBLC from normal cows might be useful in the prevention of HRV-induced severe gastroenteritis in immunocompromised hosts.

A Preparation of Cow's Late Colostrum Fraction Containing αs1-Casein Promoted the Proliferation of Cultured Rat Intestinal IEC-6 Epithelial Cells

“…Recently, we reported that skimmed sterilized, concentrated bovine colostrum (SCBLC) from healthy lactating cows of a Holstein herd at 6-7 d post-parturition was effective at preventing and fostering early recovery from human rotavirus (HRV)-induced diarrhea in suckling mice, indicating the potential of SCBLC to be used as a prophylactic food additive against HRV infection. 10) We also observed that SCBLC stimulated the proliferation of immature epithelial cells in the intestinal crypt of the small intestine in mice when fed for 1 week. 11) Thereafter, with regard to indomethacin-induced enteropathy, both pre-feeding and postfeeding with SCBLC facilitated the growth of intestinal villi.…”

“…19,20) Whey proteins consist largely of two proteins, -lactoglobulin and -lactalbumin. 10) In the present study, we examined the effect of SCBLC by the WST-1 method on the proliferation of rat intestinal epithelial IEC6-cells. The results indicate that a fraction containing s1-casein was the active ingredient in SCBLC in the stimulation of cell growth.…”

Production and Functional Properties of Dairy Products Containing Lactophorin and Lactadherin

“…Unfortunately, the clinical use of bovine colostrum from hyper-immunized cows has been limited because of difficulties in largescale production. Recently, skimmed and concentrated bovine late colostrum (SCBLC) obtained from normal cows at 6-7 days after parturition exhibited high potency in inhibiting human rotaviral replication in vitro and in vivo (Inagaki et al, 2010a), indicating that SCBLC is likely to play an alternative role to colostrum of cows hyper-immunized with rotavirus.…”

An Assay for Detecting Neutralization of Rotavirus Infection by Quantitative Determination of VP6 Protein Fluorescence Intensity

“…1) Consequently, researchers have continued to investigate safe and effective inhibitors of infection, and many previous studies have suggested compounds to prevent infection. [6][7][8][9] Previously published rotavirus infection studies used the focus reduction assay extensively, is a method that involves staining infected cells with a fluorescenceconjugated antibody and counting the stained cells by means of fluorescence microscopy. For example, the embryonic rhesus monkey kidney epithelial MA104 cell line was inoculated with a mixture of sample protein and virus, the infected cells were stained by indirect immunoantibody assay, and they were finally examined microscopically to identify immunoperoxidase-stained foci, which indicate infection.…”

“…For example, the embryonic rhesus monkey kidney epithelial MA104 cell line was inoculated with a mixture of sample protein and virus, the infected cells were stained by indirect immunoantibody assay, and they were finally examined microscopically to identify immunoperoxidase-stained foci, which indicate infection. [7][8][9] A major limitation of this method is that it fails to provide information about the underlying cells. Furthermore, to do a focus reduction assay, considerable skill is required for accurate discrimination of infected cells from noninfected cells, which typically takes a long time to perform since it relies on manual counting.…”