<i>In vitro</i> and <i>in vivo</i> antithrombotic and cytotoxicity effects of ferulic acid

Choi, Jong-In; Park, Jong Kook; Kim, Ki-Man; Lee, Hyo‐Jeong; Kim, Seung

doi:10.1002/jbt.22004

Cited by 80 publications

(54 citation statements)

References 40 publications

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“…Ferulic acid at the concentration of 300 µL/mL showed no significant cytotoxicity. Additionally, the inhibition of platelet aggregation and an anticoagulant effect was observed [113]. Ferulic acid is a free radical scavenger and inhibits the activity of the enzymes responsible for free radical generation catalysis.…”

Section: Collagen-ferulic Acidmentioning

confidence: 99%

Collagen-Based Materials Modified by Phenolic Acids—A Review

Kaczmarek

Mazur

2020

Materials

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Collagen-based biomaterials constitute one of the most widely studied types of materials for biomedical applications. Low thermal and mechanical parameters are the main disadvantages of such structures. Moreover, they present low stability in the case of degradation by collagenase. To improve the properties of collagen-based materials, different types of cross-linkers have been researched. In recent years, phenolic acids have been studied as collagen modifiers. Mainly, tannic acid has been tested for collagen modification as it interacts with a polymeric chain by strong hydrogen bonds. When compared to pure collagen, such complexes show both antimicrobial activity and improved physicochemical properties. Less research reporting on other phenolic acids has been published. This review is a summary of the present knowledge about phenolic acids (e.g., tannic, ferulic, gallic, and caffeic acid) application as collagen cross-linkers. The studies concerning collagen-based materials with phenolic acids are summarized and discussed.

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Section: Collagen-ferulic Acidmentioning

confidence: 99%

Collagen-Based Materials Modified by Phenolic Acids—A Review

Kaczmarek

Mazur

2020

Materials

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“…It exerts antitumor activity and inhibits metastasis of breast cancer cells by regulating epithelial to mesenchymal transition, and it exerts neuroprotective effects against cerebral ischemia/reperfusion‐induced injury via antioxidant and antiapoptotic mechanisms . Recently, FA was also shown to inhibit the clot retraction responsible for platelet development . However, the difference between the influence on the cell and the living body due to the introduction of a bromide onto FA is unclear.…”

Section: Resultsmentioning

confidence: 99%

“…15 Recently, FA was also shown to inhibit the clot retraction responsible for platelet development. 16 However, the difference between the influence on the cell and the living body due to the introduction of a bromide onto FA is unclear. Therefore, we investigated the cytotoxicity of FA and 6-BFA by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay at concentrations of 1, 10, 50, 100, and 1000 μM against PC12 cells.…”

Section: In Vitro Assay Of Fa and 6-bfamentioning

confidence: 99%

Characterization of 6‐bromoferulic acid as a novel common‐use matrix for matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Kato

Hirosawa

Sakamoto

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Ferulic acid (FA) is a standard matrix used for analyzing proteins. In this study, the ability of a halogenated FA to serve as an effective MALDI matrix was investigated. Various halogenated FAs were synthesized, and the characteristics and performance of each were compared with those of the standard matrices α‐cyano‐4‐hydroxycinnamic acid (CHCA) and 2,5‐dihydrobenzoic acid (DHBA). Methods The abilities of 6‐bromoferulic acid (6‐BFA), ferulic acid (FA), and eight other halogenated FA derivatives to ionize eight synthetic peptides were examined. Absorption measurements, MM2 structure optimizations, and proton affinity (PA) calculations were also performed for 6‐BFA and FA. The suitabilities of these compounds as matrices for matrix‐assisted laser desorption/ionization (MALDI) for lipids, sugar chains, polymers, cyanocobalamin, synthetic peptides, and tryptic peptides originating from two types of serum proteins were also tested. Results The 6‐position of FA was found to be the best site for introducing a bromine because the generated compound allowed facile detection of cyanocobalamin and several peptides. 6‐BFA exhibited good sensitivity for large peptides (3–5 kDa) and peptides containing acidic amino acids or proline. 6‐BFA was also shown to be a suitable matrix for tandem mass spectrometry (MS/MS) analysis when using MALDI time‐of‐flight (TOF) mass spectrometry (MS) with a quadrupole ion trap (QIT) system. Conclusions The properties of 6‐BFA as a MALDI matrix differed from those of DHBA and CHCA. 6‐BFA appears to be a useful matrix for de novo sequencing using MALDI‐QIT‐TOF‐MS.

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“…Choi et al . ( 38 ) found that a ferulate treatment up to 1.54 mM did not show any toxicity on NIH-3T3, HepG2, or 3T3-L1 cells. Additionally, 5.1 mM ferulate treatment for 7 days did not exhibit toxicity on human bone marrow stromal cells ( 39 ).…”

Section: Discussionmentioning

confidence: 97%

Ferulate, an Active Component of Wheat Germ, Ameliorates Oxidative Stress-Induced PTK/PTP Imbalance and PP2A Inactivation

et al. 2018

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Ferulate is a phenolic compound abundant in wheat germ and bran and has been investigated for its beneficial activities. The aim of the present study is to evaluate the efficacy of ferulate against the oxidative stress-induced imbalance of protein tyrosine kinases (PTKs), protein tyrosine phosphatases (PTPs), and serine/threonine protein phosphatase 2A (PP2A), in connection with our previous finding that oxidative stress-induced imbalance of PTKs and PTPs is linked with proinflammatory nuclear factor-kappa B (NF-κB) activation. To test the effects of ferulate on this process, we utilized two oxidative stress-induced inflammatory models. First, YPEN-1 cells were pretreated with ferulate for 1 hr prior to the administration of 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH). Second, 20-month-old Sprague-Dawley rats were fed ferulate for 10 days. After ferulate treatment, the activities of PTKs, PTPs, and PP2A were measured because these proteins either directly or indirectly promote NF-κB activation. Our results revealed that in YPEN-1 cells, ferulate effectively suppressed AAPH-induced increases in reactive oxygen species (ROS) and NF-κB activity, as well as AAPH-induced PTK activation. Furthermore, ferulate also inhibited AAPH-induced PTP and PP2A inactivation. In the aged kidney model, ferulate suppressed aging-induced activation of PTKs and ameliorated aging-induced inactivation of PTPs and PP2A. Thus, herein we demonstrated that ferulate could modulate PTK/PTP balance against oxidative stress-induced inactivation of PTPs and PP2A, which is closely linked with NF-κB activation. Based on these results, the ability of ferulate to modulate oxidative stress-related inflammatory processes is established, which suggests that this compound could act as a novel therapeutic agent.

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In vitro and in vivo antithrombotic and cytotoxicity effects of ferulic acid

Cited by 80 publications

References 40 publications

Collagen-Based Materials Modified by Phenolic Acids—A Review

Collagen-Based Materials Modified by Phenolic Acids—A Review

Characterization of 6‐bromoferulic acid as a novel common‐use matrix for matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Ferulate, an Active Component of Wheat Germ, Ameliorates Oxidative Stress-Induced PTK/PTP Imbalance and PP2A Inactivation

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