<i>In Vitro</i> and <i>in Vivo</i> Demonstration of Photodynamic Activity and Cytoplasm Imaging through TPE Nanoparticles

Jayaram, Dhanya T.; Ramos-Romero, Sara; Shankar, Balaraman H.; Garrido, Cristina; Rubio, Núria; Sánchez-Cid, Lourdes; Borrós, Salvador; Blanco, José L. Jiménez; Ramaiah, Danaboyina

doi:10.1021/acschembio.5b00537

Cited by 53 publications

(31 citation statements)

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“…To achieve the mitochondrial targeting, various cationic lipophilic fluorescent dyes, attracted to mitochondria due to large values of the membrane potential (ΔΨ m ), have been developed. The ΔΨ m value is a critical parameter reflecting the mitochondrial functional status and cell viability [54]. However, the photostability of these dyes is extremely low due to the effect of concentration on the emission quenching [55].…”

Section: Photosensitizersmentioning

confidence: 99%

“…The using of TPE conjugated with dicyanovinyl promoted the production of high twophoton absorption cross section, thereby a promising two-photon imaging technology was established [57]. The conjugation of TPE with benzothiazole derivatives, which facilitates the self-assembly with NPs, was shown to be effective towards PC3 human prostate cancer cells, both in vitro and in vivo [54].…”

Section: Photosensitizersmentioning

confidence: 99%

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Nanoparticles-based photosensitizers with effect of aggregation-induced emission

Korneev¹,

Сахно

Короткова

2019

Biopolym. Cell

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Photodynamic therapy (PDT) is a method of the treatment of localized cancers, based on a photochemical reaction between a light-activated molecule or photosensitizer (PS), light, and molecular oxygen. Correct choice of PS is of fundamental importance for PDT efficacy. Despite numerous studies in this field, most known PS have some drawbacks, e.g. lack of specificity and aggregation in aqueous media. Consequently, the search for an ideal PS is essential for further development of PDT. Here we review classification and analyse main features of different generations of PS and describe the mechanisms of their action. Various methods of targeted delivery of PS to tumor cells are discussed. The advantages of PS nanoparticles with the effect of aggregation-induced emission (AIE) over the classic photosensitizers are presented. A possibility of practical application of such light-emitting structures in cancer phototherapy is shown. K e y w o r d s: photodynamic therapy (PDT), photosensitizer, aggregation-induced emission ABBrEVIATIONS AEMA -2-aminoethyl methacrylate ALA -5-aminolevulinic acid AlPc -aluminum-phthalocyanine chloride AlPcS4 -aluminium-phthalocyanine tetrasulphonated (Photosens) AlS2Pc -disulfonated phthalocyanine BDP -borondipyrromethene BHQ3 -black hole quencher3 BSA -bovine serum albumin c(rGDfc) -Arg-Gly-Asp-d-Phe-Cys cyclicpeptide crGD -cyclic arginine-glycine-aspartic acid DCF-DA -dichlorofluoresceindiacetate DMSO -Dimethylsulfoxide DPBA-TPE -(3,3'-(2,5-dimethoxy-1,4-phenylene)bis(2-(4bromophenyl)acrylonitrile)-tetraphenylethene DSPE-PEG-Mal -1,2-distearoyl-sn-glycero3-phosphoethanolamine-N-[maleimide(polyethylene glycol)] DTPEBBTD -Donor-tetraphenylethene-benzo[1,2-c:4,5-c']bis([1,2,5]thiadiazole) Fr/NIr -far-red/ near-infrared GFLG -Gly-Phe-Leu-Gly-peptide HPMA -N-(2-hydroxypropyl)methacrylamide m-THPP -meta-tetra(hydroxyphenyl)porphyrin (Foscan)

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Section: Photosensitizersmentioning

confidence: 99%

Section: Photosensitizersmentioning

confidence: 99%

Nanoparticles-based photosensitizers with effect of aggregation-induced emission

Korneev¹,

Сахно

Короткова

2019

Biopolym. Cell

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“…Tetraphenylethene nanoparticles showed a maximum absorption of 450 nm with a maximum emission of approximately 700 nm. 166 Another study investigated PEGylated graphene oxide nanoparticles with encapsulated sinoporphyrin sodium (DVDMS). These nanoparticles exhibited increased accumulation in cancer cells than DVDMS alone.…”

Section: Polymer-core and Fluorophore Nanoparticlesmentioning

confidence: 99%

Mini-review: fluorescence imaging in cancer cells using dye-doped nanoparticles

2016

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Fluorescence imaging has gained increased attention over the past two decades as a viable means to detect a variety of cancers. Fluorescence imaging has the potential to provide physicians with high resolution images with enhanced contrast, which will allow them to be able to better diagnose and treat patients with cancer. Early detection and treatment are key to erradicating cancer in a patient, and fluorescence imaging has the ability to identify non-advanced, even pre-cancerous, tumors where imaging based on white light or radiation overlooked them. Several fluorescent dyes have been identified as possible fluorophores for enhanced fluorescence imaging, such as cyanine, squaraine, porphyrin, phthalocyanine, and borondipyrromethane dyes. These dyes have high fluorescence quantum yields, which provides a high target to background ratio; however, these dyes are often plagued by low water solubility. This low solubility can be ameliorated by conjugating or covalently attaching these dyes to polymeric crosslinked micelles, polymersomes, or polymer-core nanoparticles. These particle & dye systems then can become platforms on which secondary components can be attached to enhance the systems functionality. For example, dyes attached to these nanocarriers can target tumors through passive targeting; however, active targeting can be achieved by further modifying these nanocarriers with ligands that have a binding affinity for receptors overexpressed in tumor cells, cell surface receptors located on the tumor cell membrane, or endothelium. Fluorescence activation of the probes is another promising technology for the early detection of cancer. Activation requires that there be a change in fluorescence, whether it be an emission wavelength change or a fluorescence "on/off" signal when in the presence of some external stimuli. Activation increase the target to background ratio and enhances the contrast of the obtained image. This review serves to highlight the recent developments of (1) improved fluorescent dyes for the detection of cancer, with a specific focus on dyes that are being coupled to nanocarriers; (2) dye & nanocarrier systems that target, both actively and passively, tumors, and (3) fluorescence activation of these fluorophore systems for better image quality.

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“…In the field of photodynamic therapy, AIEgens have been reported to be combined with commonly used PSs, such as PpIX, acting as a sensing probe [42] or an enhancer for ROS production [43,44]. AIE nanoparticles have also been used only as a PS (not a fluorophore) to achieve in vivo PDT via intratumoral injection [45].…”

mentioning

confidence: 99%

Targeted and imaging-guided in vivo photodynamic therapy for tumors using dual-function, aggregation-induced emission nanoparticles

et al. 2018

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Imaging-guided photodynamic therapy (PDT) has been regarded as a promising strategy for precise cancer treatment. Because of their excellent modifiability and drug loading capacity, nanoparticles have played an important role in PDT. However, when traditional photosensitizers are made into nanoparticles, both their fluorescence and reactive oxygen species (ROS) generation efficiency are decreased due to a phenomenon known as aggregationcaused quenching. Fortunately, in recent years, several kinds of organic dyes have been developed with "abnormal" properties termed aggregation-induced emission (AIE). With enhanced fluorescence emission in the nano-aggregation state, the traditional obstacles mentioned above could be overcome by AIE luminogens (AIEgens). Herein, we provide a better combination of photosensitizers and nanoparticles, a kind of dual-functional AIE nanoparticle capable of producing ROS, to achieve targeted and imaging-guided in vivo PDT.Good contrast in in vivo imaging and obvious therapeutic efficiency were realized with a low dose of AIE nanoparticles as well as a low power density of light, resulting in negligible side effects. Our work demonstrates that AIE nanoparticles could play a promising role for imagingguided clinical photodynamic cancer therapy in the near future.Cancer has increasingly become a primary threat to human health, and more efficient treatment methods are urgently needed [1][2][3][4][5]. Conventional cancer treatment methods, including surgery, chemotherapy, and radiotherapy, lack accuracy and have significant side effects [6][7][8][9][10]. Thus, much attention has been paid to the development of alternative novel treatment modalities [11][12][13][14][15]. Imaging-guided photodynamic therapy (PDT) has been developed and shown to be an efficient, precise, and non-invasive medical technique for cancer therapy [16][17][18][19][20]. The photodynamic effect consists of three elements: a photosensitizer (PS), light of a suitable wavelength, and oxygen. Briefly, the energy of the light can be utilized to transfer the non-toxic triplet oxygen to toxic reactive oxygen species (ROS). Because the process occurs only when both the PS and light of a particular wavelength are present together, this method can provide good selectivity for the treatment region [21][22][23]. Meanwhile, most PSs demonstrate fluorescence emission [23,24], and with the guidance of fluorescence images, PDT can be a rather precise treatment modality.Along with the development of nanotechnology, various series of colloidal nanoparticles have become powerful tools in PDT [25][26][27][28]. Since the nanometer scale provides a high surfaceto-volume ratio, nanoparticles can ensure high drug-loading capacity as well as efficient surface chemical modification. A high drug-loading capacity leads to fewer side effects, while surface chemical modification allows for customized designs, which can greatly improve the specific properties of drugs, such as hydrophilicity [29], targeting ability [30][31][32][33] etc. In addition, ...

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In Vitro and in Vivo Demonstration of Photodynamic Activity and Cytoplasm Imaging through TPE Nanoparticles

Cited by 53 publications

References 54 publications

Nanoparticles-based photosensitizers with effect of aggregation-induced emission

Nanoparticles-based photosensitizers with effect of aggregation-induced emission

Mini-review: fluorescence imaging in cancer cells using dye-doped nanoparticles

Targeted and imaging-guided in vivo photodynamic therapy for tumors using dual-function, aggregation-induced emission nanoparticles

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