<i>In vitro</i> antagonistic activity of <i>Trichoderma</i> spp. against fungal pathogens causing black point disease of wheat

Yassin, Mohamed Taha; Mostafa, Ashraf Abdel‐Fattah; Al-Askar, Abdulaziz A.

doi:10.1080/16583655.2022.2029327

Cited by 27 publications

(10 citation statements)

References 51 publications

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“…Cultural filtrates of the antagonistic fungal strains Trichoderma harzianum and Trichoderma viride showed antifungal potency against different pathogenic fungal strains [ 56 ]. The culture filtrate of Trichoderma longibrachiatum SFC100166 could be a valuable source for the enhancement of natural agents to control late blight caused by Phytophthora infestans [ 57 ].…”

Section: Resultsmentioning

confidence: 99%

“…The best effect in suppressing cantaloupe root rot was shown by the growth of plants treated with Trichoderma spp., which was greater than the growth of those under the pathogen alone. In a pot culture assay, it was discovered that adding Cultural filtrates of the antagonistic fungal strains Trichoderma harzianum and Trichoderma viride showed antifungal potency against different pathogenic fungal strains [56]. The culture filtrate of Trichoderma longibrachiatum SFC100166 could be a valuable source for the enhancement of natural agents to control late blight caused by Phytophthora infestans [57].…”

Section: Growth Parametersmentioning

confidence: 99%

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Polyphenols-Rich Extract of Calotropis procera Alone and in Combination with Trichoderma Culture Filtrate for Biocontrol of Cantaloupe Wilt and Root Rot Fungi

Nofal,

Hamouda,

Rizk

et al. 2023

Molecules

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Fungal diseases have always been a major problem for cantaloupe crops; however, synthetic fungicides are hazardous to humans and the environment. Consequently, a feasible alternative to fungicides without side effects could be by using bio agents and naturally occurring plants with antibacterial potential. This study has achieved a novel procedure for managing wilt and root rot diseases by potentially using Trichoderma sp. culture filtrates in consortium with plant extract of Calotropis procera, Rhizoctonia solani, Fusarium oxysporum, and Pythium ultimum, which were isolated from infected cantaloupe roots with identified root rot symptoms. The antagonistic activity of four Trichoderma isolates and analysis of antibiotics and filtrate enzymes of the most active Trichoderma isolate were determined as well as phytochemical analysis of C. procera plant extract using HPLC-UV. The obtained results showed that all Trichoderma isolates considerably lowered the radial growth of P. ultimum, R. solani, and F. oxysporum in varying degrees. The scanning electron micrographs illustrate the mycoparasitic nature of Trichoderma sp. on F. oxysporum. The phytochemical analysis of C. procera indicated that phenolic contents were the major compounds found in extracts, such as vanillin (46.79%), chlorogenic acid (30.24%), gallic acid (8.06%), and daidzein (3.45%) but including only a low amount of the flavonoid compounds rutin, naringenin, and hesperetin. The Pot experiment’s findings showed that cantaloupe was best protected against wilting and root rot diseases when it was treated with both Trichoderma sp. culture filtrates (10%) and C. procera extract of (15 mg/mL), both alone and in combination. This study demonstrates that the application of bio agent Trichoderma spp. filtrate with C. procera phenol extract appears useful for controlling wilting and root rot disease in cantaloupe. This innovative approach could be used as an alternative to chemical fungicide for the control of wilting and rot root diseases.

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Section: Resultsmentioning

confidence: 99%

Section: Growth Parametersmentioning

confidence: 99%

Polyphenols-Rich Extract of Calotropis procera Alone and in Combination with Trichoderma Culture Filtrate for Biocontrol of Cantaloupe Wilt and Root Rot Fungi

Nofal,

Hamouda,

Rizk

et al. 2023

Molecules

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“…The attack on pathogenic fungi involving conidia and coiling hyphae could be prompted by organic compounds and/or nutrient gradients emanating from the pathogens and sensed by Trichoderma 92 . Meanwhile, Trichoderma harzianum and T. viride strains performed best in a dual culture experiment against A. alternata with inhibition percentages of 75.04 and 67.83%, respectively 93 . Additionally, the hyphal penetration of T. harzianum into the hyphae of A. alternata , F. proliferatum and B. sorokiniana was demonstrated to be another mycoparasitic mechanism of action 94 , 95 .…”

Section: Discussionmentioning

confidence: 98%

Trichoderma afroharzianum TRI07 metabolites inhibit Alternaria alternata growth and induce tomato defense-related enzymes

Philip,

Behiry,

Salem

et al. 2024

Sci Rep

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Identifying a viable substitute for the limited array of current antifungal agents stands as a crucial objective in modern agriculture. Consequently, extensive worldwide research has been undertaken to unveil eco-friendly and effective agents capable of controlling pathogens resistant to the presently employed fungicides. This study explores the efficacy of Trichoderma isolates in combating tomato leaf spot disease, primarily caused by Alternaria alternata. The identified pathogen, A. alternata Alt3, was isolated and confirmed through the ITS region (OQ888806). Six Trichoderma isolates were assessed for their ability to inhibit Alt3 hyphal growth using dual culture, ethyl acetate extract, and volatile organic compounds (VOCs) techniques. The most promising biocontrol isolate was identified as T. afroharzianum isolate TRI07 based on three markers: ITS region (OQ820171), translation elongation factor alpha 1 gene (OR125580), and RNA polymerase II subunit gene (OR125581). The ethyl acetate extract of TRI07 isolate was subjected to GC–MS analysis, revealing spathulenol, triacetin, and aspartame as the main compounds, with percentages of 28.90, 14.03, and 12.97%, respectively. Analysis of TRI07-VOCs by solid-phase microextraction technique indicated that the most abundant compounds included ethanol, hydroperoxide, 1-methylhexyl, and 1-octen-3-one. When TRI07 interacted with Alt3, 34 compounds were identified, with major components including 1-octen-3-one, ethanol, and hexanedioic acid, bis(2-ethylhexyl) ester. In greenhouse experiment, the treatment of TRI07 48 h before inoculation with A. alternata (A3 treatment) resulted in a reduction in disease severity (16.66%) and incidence (44.44%). Furthermore, A3 treatment led to improved tomato growth performance parameters and increased chlorophyll content. After 21 days post-inoculation, A3 treatment was associated with increased production of antioxidant enzymes (CAT, POD, SOD, and PPO), while infected tomato plants exhibited elevated levels of oxidative stress markers MDA and H2O2. HPLC analysis of tomato leaf extracts from A3 treatment revealed higher levels of phenolic acids such as gallic, chlorogenic, caffeic, syringic, and coumaric acids, as well as flavonoid compounds including catechin, rutin, and vanillin. The novelty lies in bridging the gap between strain-specific attributes and practical application, enhancing the understanding of TRI07’s potential for integrated pest management. This study concludes that TRI07 isolate presents potential natural compounds with biological activity, effectively controlling tomato leaf spot disease and promoting tomato plant growth. The findings have practical implications for agriculture, suggesting a sustainable biocontrol strategy that can enhance crop resilience and contribute to integrated pest management practices.

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“…Biological management with bacteria, fungi, and other predators is a common technique. Fungi are the most diversified natural enemies of pathogenic fungi, and they utilize a range of ways to eliminate pathogenic fungi [62][63][64][65][66]. Trichoderma species are well adapted to survive various stress conditions.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol of Trichoderma gamsii induces soil suppressive and growth‐promoting impacts and rot disease‐protecting activities

Arasu

Vijayaraghavan²,

Al-Dhabi

et al. 2023

J Basic Microbiol

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Plant growth-promoting rhizosphere of associated fungi is often used to improve productivity and crop health. Some biocontrol fungi showed less disease protection efficiency due to environmental factors. Hence, the analysis of biocontrol fungi strains in the field is valuable to analyze their performance in the natural environment. The main objective of this study is to isolate the growth-promoting, rot disease-protecting activities of Trichoderma gamsii RH4 obtained from the rhizosphere of black gram (Vigna mungo). In this study, we evaluated the protective role of 13 fungal strains, including T. gamsii against Fusarium oxysporum in the laboratory experiment (antagonistic assay), greenhouse. The growth-promoting activity of the isolated 13 fungal strains was initially screened and the lytic property was assayed. The T. gamsii showed excellent growth-promoting and lytic properties. Lytic enzyme assays revealed a significant difference in the enzyme yield than other isolates (p < 0.05). The influence of selected plant-growth-promoting fungal strains on the suppression of F. oxysporum rot and wilt black gram was analyzed. Results revealed that these fungal isolates improved black gram growth in greenhouse and significantly reduced wilt and rot disease. The improved growth and yield registered in this study proved growth-promoting and biocontrol properties.The potent T. gamsii was applied in the open field and its effect on green gram was observed. The field trial experiment revealed several growth-promoting effects such as the weight and length of the root and shoot system. The isolated native T. gamsii has the potential to improve the biocontrol properties against rot disease.

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In vitro antagonistic activity of Trichoderma spp. against fungal pathogens causing black point disease of wheat

Cited by 27 publications

References 51 publications

Polyphenols-Rich Extract of Calotropis procera Alone and in Combination with Trichoderma Culture Filtrate for Biocontrol of Cantaloupe Wilt and Root Rot Fungi

Polyphenols-Rich Extract of Calotropis procera Alone and in Combination with Trichoderma Culture Filtrate for Biocontrol of Cantaloupe Wilt and Root Rot Fungi

Trichoderma afroharzianum TRI07 metabolites inhibit Alternaria alternata growth and induce tomato defense-related enzymes

Biocontrol of Trichoderma gamsii induces soil suppressive and growth‐promoting impacts and rot disease‐protecting activities

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