In Vitro Anti-tumoral and Anti-bacterial Activity of an Octamolybdate Cluster-Based Hybrid Solid Incorporated with a Copper Picolinate Complex

Abstract

Inorganic drugs, especially polyoxometalate-based hybrids, are expected to be developed as promising future metallodrugs. Herein, an organic–inorganic hybrid solid based on pyridine-2-carboxylic acid or picolinic acid (pic), [(Cu­(pic)2)2(Mo8O26)]·8H2O (1), was synthesized. A single-crystal structure of a solid possesses a discrete β-type octamolybdate cluster that supramolecularly aggregates with a {Cu2(pic)4}4– complex and eight lattice water molecules. The study indicates that the solid is stable in aqueous medium and less toxic toward normal cell lines. The in vitro anti-bacterial and anti-tumor properties of the solid 1 were investigated. The results of the anti-tumor action against various human cancer cell lines, namely, lung (A549), breast (MCF-7), and liver (HepG2) cancer cells suggest that this β-octamolybdate-based solid yielded the lowest IC50 value reported so far among octamolybdate anion-based hybrid solids, i.e., 24.24 μM for MCF-7, 21.56 μM for HepG2, and 25 μM for A549, indicating significant anti-cancer activity. The cell cycle analysis further reveals the observed anti-tumor effect to be governed by the arrest of breast cancer cells in the G2/M phase while that of lung and liver cancer cells in the S phase of the cell cycle. A fluorescence quenching study suggests the binding interaction between solid and ctDNA, which in turn induces apoptosis and necrosis pathways leading to cancer cell death. This is also the first study of {Mo8O26}4– cluster-based solids as an anti-bacterial agent against Escherichia coli, and it was found to be very effective with a minimal inhibitory concentration value of ∼135 μg/mL, which is the lowest so far reported for any octamolybdate-based solid.

Fabrication of novel Fe (III), Co (II), Hg (II), and Pd (II) complexes based on water‐soluble ligand (NaH2PH): structural characterization, cyclic voltammetric, powder X‐ray diffraction, zeta potential, and biological studies

“…In vitro anticancer potencies of the isolated Schiff base (NaH 2 PH) ligand and Co (II), Fe (III), Hg (II), and Pd (II) complexes were evaluated by MTT assay against (HePG-2) liver and (MCF-7) breast cancer cell lines. [22,23] Briefly, 100-μl cell suspension containing 2.5 Â 10 3 cells/well was seeded in 96-well culture plate. The (HePG-2) and (MCF-7) cells were incubated at 37 C for 24 h in 5% CO 2 conditions for settle down and adherence.…”

A polyoxomolybdate-based hybrid nano capsule as an antineoplastic agent

“…1. 6,38,39,41,42,[44][45][46] The elemental questions regarding the mechanism behind POM-mediated antiproliferative activity are still up for debate. However, it is well accepted that POMs can show cancer cell penetration as their location has been experimentally conrmed within cytoplasmic space.…”

“…11,51 So in some of the reports DNA interaction with POM was elucidated to be the mechanism of anticancer activity. 6,11,48,52,53 Few more mechanisms of anti-tumor activity were found to be inhibition of angiogenesis and interaction of polyoxometalate with proteins. 4,6…”

“…6,11,48,52,53 Few more mechanisms of anti-tumor activity were found to be inhibition of angiogenesis and interaction of polyoxometalate with proteins. 4,6…”

Polyoxometalate-Derived Cu-MoO2 Nanosheets as Electrocatalysts for Enhanced Acidic Water Oxidation

“…The precursor octamolybdate-based POM chosen for this study was earlier synthesized in our lab. 36 The catalyst was prepared by annealing the precursor [(Cu(pic) 2 ) 2 (Mo 8 O 26 )]•8H 2 O (POM) at 500 °C for 2 h in Ar gas atmosphere (Scheme 1). The resultant nanosheet-like catalyst [Cu 0.1 -{MoO 2 } 0.9 ] (PS-55) was cooled to room temperature in the Ar flow.…”