In vitroantibacterial effect of berberine hydrochloride and enrofloxacin to fish pathogenic bacteria

Abstract

Berberine the main antibacterial substance in the rhizome of Coptis chinensis Franch, is used as an antimicrobial agent for a long time. This study was carried out to investigate the potential of berberine alone or in combination with enro£oxacin against six common ¢sh pathogens for use in ¢sh disease management in aquaculture. The minimum inhibitory concentrations (MICs) of berberine hydrochloride against Aeromonas hydrophila, Pseudomonas £uorescens, Vibrio vulni¢cus, Edwardsiella ictaluri, Escherichia coli and Streptococcus agalactiae were determined and to be 4500, 4500, 4500, 300, 400 and 100 mg mL À 1 respectively. The minimal bactericidal concentrations (MBCs) of berberine hydrochloride to E. coli, E. ictaluri and S. agalactiae were 300^500 mg mL À 1. In combination with enro£oxacin, the MICs and MBCs of berberine hydrochloride signi¢cantly decreased against E. coli and E. ictaluri but not against Streptococcus dysgalactiae. These results demonstrated that the berberine hydrochloride enhanced the bactericidal e¡ect of enro£oxacin and vice versa. The synergistic bactericidal e¡ect of berberine hydrochloride and enro£oxacin suggest its potential use in ¢sh disease management in aquaculture. This is the ¢rst study on the inhibitory e¡ect of berberine hydrochloride against ¢sh pathogenic bacteria.

Antibiotic Use by Small‐Scale Farmers for Freshwater Aquaculture in the Upper Mekong Delta, Vietnam

“…; Zhang et al. ); however, its major use has been in poultry production. Enrofloxacin is rapidly metabolised to ciprofloxacin (Anadon et al.…”

Integrated pharmacokinetics/pharmacodynamics parameters-based dosing guidelines of enrofloxacin in grass carp Ctenopharyngodon idella to minimize selection of drug resistance

“…Until now, antibacterial drugs through oral delivery are the popular and effective choice for the control of pandemics causing by A. hydrophila . In China, enrofloxacin is one of the effective antimicrobials in grass carp farms and has been widely applied in fish ponds nationwide for over 10 years [7]. …”

Does the gastrointestinal tract serve as the infectious route ofAeromonas hydrophilain crucian carp (Carassius carassius)?

“…Aeromonas hydrophila, an opportunistic pathogen, is prevalent in aquatic habitats with cosmopolitan distribution and has resulted in heavy mortalities in farmed and feral fish (Janda & Abbott 1998;Harikrishnan & Balasundaram 2005; Da Silva, Mourino, Vieira, Jatoba, Seiffert & Martins 2012). As a major aquaculture country, China accounts for nearly 70% of the world's total aquaculture production (Zhang, Li, Xie & Ji 2010); however, MAS caused by A. hydrophila has frequently caused huge economical loss in cyprinid fish industry throughout China since the 1980s (Nielsen et al 2001). Clarifying the infectious route of A. hydrophila in fish and finding an efficient measure to prevent or treat this disease are of utmost importance.…”