In vitro
 anticancer, antioxidant and enzyme inhibitory potentials of endemic
 Cephalaria elazigensis
 var.
 purpurea
 with
 in silico
 studies

Erdoğan, Mehmet Kadir; Gündoğdu, Ramazan; Yapar, Yakup; Geçibesler, İbrahim Halil; Kırıcı, Mahinur; Behçet, Lütfi; Tüzün, Burak; Tok, Tuğba Taşkın; Taslimi, Parham

doi:10.1080/07391102.2022.2163700

Cited by 11 publications

(4 citation statements)

References 53 publications

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“…The HPLC-MS/MS analysis of Nepeta species was explained in detail in our previously published article, and the same method was used in this study. [35] The GC-FID analysis of extracts was made in an Agilent 7820 A GC-FID. The procedure was set to an initial temperament of 100 °C for 5 minutes, a ramp at 4 °C/min to 240 °C, and 20 minutes at 240 °C, respectively.…”

Section: Profile Of Chemical Compositionmentioning

confidence: 99%

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In Vitro Bioactivities and In Silico ADME Profile for Potential Biological Target Prediction of Selected Phytochemicals Using Six Nepeta Species

Yenigun,

Basar,

Gül

et al. 2024

ChemistrySelect

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Several Nepeta species, used in traditional uses, represent sources of valuable phytochemical composition, as well as in vitro biological and in silico clinical activities. In the present study, the antioxidant and DNA protective properties of methanol: chloroform (1 : 1) extracts of six Nepeta species were evaluated by comparison with standards. In HPLC‐MS/MS analysis, the main components of the extracts were identified as rosmarinic acid, caffeic acid, chlorogenic acid, shikimic acid, and scutellarin. In the GC‐FID analysis, the highest components were palmitate and cis‐11‐eicosenoic acid. N. aristata has the greatest total phenol and flavonoid contents. N. baytopii, N. aristata, N. italica, N. stenantha, and N. nuda exhibited the highest antioxidant activities. The N. nuda, N. aristata, N. italica, and N. trachonitica extracts were shown to have the most potent DNA protection activity. According to the result of the GC‐FID and HPLC‐MS/MS analysis of Nepeta extracts, ADMET studies of the main components of the extracts were performed. It was evaluated that palmitic acid had BBB permeability, but oleic acid was toxic. The in vitro and in silico results confirmed that Nepeta L. extracts are potential natural products.

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Section: Profile Of Chemical Compositionmentioning

confidence: 99%

“…The extraction process of Nepeta species was explained in detail in our previously published article, and the same method was used in this study. [35]…”

Section: Experimental Section Extraction and Plant Materialsmentioning

confidence: 99%

In Vitro Bioactivities and In Silico ADME Profile for Potential Biological Target Prediction of Selected Phytochemicals Using Six Nepeta Species

Yenigun,

Basar,

Gül

et al. 2024

ChemistrySelect

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“…Biologically active compounds reduce enzyme activity by binding to the active site of target proteins. [11][12][13][14] Herbal products have conventionally used for curative properties and screening studies are carried out to clarify the biological activities of plants. [15][16][17] Scientific authentication of the inhibitory potency of the endemic plants has not been manifested in the literature survey.…”

Section: Introductionmentioning

confidence: 99%

“…Plant extracts and compounds have inhibitory ability and enzymes with overactivity could be controlled with inhibitory agents. Biologically active compounds reduce enzyme activity by binding to the active site of target proteins [11–14] . Herbal products have conventionally used for curative properties and screening studies are carried out to clarify the biological activities of plants [15–17] …”

Section: Introductionmentioning

confidence: 99%

Evaluation of Biological Potency of two Endemic Species Integrated with in vitro and in silico Approches: LC–MS/MS Analysis of the Plants

Kısa,

Baş Topcu,

Tunçkol

et al. 2024

Chemistry & Biodiversity

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In the present study, the main phytochemical components of endemic plant extracts and inhibitory potency were screened related to different biological activities. Seven compounds were quantified, and cyanidin‐3‐o‐glucoside was the dominant secondary metabolite in plant extracts. The extract from P. asiae‐minoris (PAM) exhibited the best enzyme inhibitory activity against BChE (1.73±0.23 µg mLˉˡ), tyrosinase (2.47±0.28 µg mLˉˡ), α‐glucosidase (5.28±0.66 µg mLˉˡ), AChE (8.66±0.86 µg mLˉˡ), and ACE (19.27±1.02 µg mLˉˡ). In vitro antioxidant assay, PAM extract possessed the highest activity in respect of DPPH radical scavenging (24.29±0.23 µg/mL), ABTS•+ scavenging (13.50±0.27 µg/mL) and FRAP reducing power (1.56±0.01 μmol TE/g extract). MIC values ranged from 1 ‐ 8 mg/mL for antibacterial ability, and the PAM extract showed a stronger effect for B. subtilis, E. faecalis, and E. coli at 1 mg/mL. The antiproliferative ability of AB extract demonstrated a suppressive effect (IC50: 70.26 µg/mL) for pancreatic cancer cell lines. According to the affinity scores, the cyanidin‐3‐o‐glucoside demonstrated the lowest docking scores against ACE, AChE, BChE, and collagenase. It was found that the PAM exhibited better inhibitory capabilities than A. bartinense. The P. asiae‐minoris, reported to be in the Critically Endangered (CR) category, should be conserved by culturing, considering its biological abilities.

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Verbascum gimgimense an Endemic Turkish Plant: Evaluation of In Vitro Anticancer, Antioxidant, Enzyme Inhibitory Activities, and Phytochemical Profile

Erdogan,

Sever,

Gundogdu

et al. 2024

Cell Biochemistry & Function

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The Verbascum genus has gained significant attention in the pharmaceutical field, particularly in recent years, due to its valuable medicinal properties, which are well‐recognized in complementary and alternative medicine. Certain species within this genus contain essential compounds and exhibit a wide range of therapeutic activities. In this study, the ethanolic extract of Verbascum gimgimense (VG) was analyzed for its cytotoxic, apoptotic, antioxidant, and enzyme inhibitory properties, as well as its phenolic and lipophilic compounds. The phenolic compounds in the extract were identified using Exactive Plus Orbitrap HPLC‐HRMS, while the lipophilic components were characterized by GC‐MS analysis. The Neutral Red Uptake (NRU) cell viability assay and colony formation assay were performed to assess the antiproliferative and anti‐colony survival effects of VG on the A549 human lung adenocarcinoma cell line. Additionally, a wound healing assay measured cell migration, and the apoptotic process was evaluated using Caspase‐3 ELISA and acridine orange/ethidium bromide staining. Protein expression levels were determined by western blot analysis. DPPH, ABTS FRAP, and CUPRAC assays were used to determine free radical scavenging, reducing power, and metal chelating activities, respectively. VG was rich in dominant phenolic components, including benzoic acid (6.809 mg/g extract), phloretic acid (1.279 mg/g extract), luteolin 7‐rutinoside (2.799 mg/g extract), luteoloside (3.300 mg/g extract), kuromanine (3.456 mg/g extract), and rutin hydrate (2.015 mg/g extract). Major fatty acids identified in VG included palmitic acid (17.3%), stearic acid (2.99%), linoleic acid (9.44%), and α‐linolenic acid (26.48%). VG treatment significantly reduced colony formation ability, decreased wound closure, and increased both apoptotic cell count and caspase‐3 activity compared to the control group. Protein levels of c‐PARP, p53, and p21 were substantially elevated compared to controls. In addition to its strong free radical scavenging, reducing power and metal chelating activity, VG exhibited strong inhibitory effects on α‐amylase, α‐glucosidase, AChE, BChE, and tyrosinase. Our study demonstrates that VG possesses antiproliferative, apoptotic, antioxidant, and enzyme‐inhibitory properties. V. gimgimense emerges as a promising natural antioxidant source with potentially significant regulatory effects on key enzymes and proteins, which could contribute to managing various human diseases and inspire the development of novel therapeutic strategies.

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In vitro anticancer, antioxidant and enzyme inhibitory potentials of endemic Cephalaria elazigensis var. purpurea with in silico studies

Cited by 11 publications

References 53 publications

In Vitro Bioactivities and In Silico ADME Profile for Potential Biological Target Prediction of Selected Phytochemicals Using Six Nepeta Species

In Vitro Bioactivities and In Silico ADME Profile for Potential Biological Target Prediction of Selected Phytochemicals Using Six Nepeta Species

Evaluation of Biological Potency of two Endemic Species Integrated with in vitro and in silico Approches: LC–MS/MS Analysis of the Plants

Verbascum gimgimense an Endemic Turkish Plant: Evaluation of In Vitro Anticancer, Antioxidant, Enzyme Inhibitory Activities, and Phytochemical Profile

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