In Vitro Antidiabetic Effects of Isolated Triterpene Glycoside Fraction from Gymnema sylvestre

Shenoy, Rashmi S.; Prashanth, K. V. Harish; Manonmani, H. K.

doi:10.1155/2018/7154702

Cited by 29 publications

(26 citation statements)

References 34 publications

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“…The efficiency of GA in inhibiting glucose absorption in the small intestine was increased by a combined effect with acarbose and voglibose [55]. GA and PC have potent inhibitory effects on α-amylase and α-glucosidase [55,56]. We hypothesize that the presence of GA II and PC in CuO NPs elicited by CSC extracts of G. sylvestre could significantly inhibit α-amylase and glycosylation activities.…”

Section: Antidiabetic Activitymentioning

confidence: 91%

“…GA obtained from G. sylvestre inhibits glucose absorption in the small intestine and suppresses hyperglycemia and hyperinsulinemia in oral glucose tolerance test. The efficiency of GA in inhibiting glucose absorption in the small intestine was increased by a combined effect with acarbose and voglibose [55]. GA and PC have potent inhibitory effects on α-amylase and α-glucosidase [55,56].…”

Section: Antidiabetic Activitymentioning

confidence: 99%

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Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br

et al. 2019

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Gymnema sylvestre is a plant that is enriched in bioactive compounds. In particular, gymnemic acids (GA) and phenolic compounds (PC) are pharmaceutically important. There is a commercial demand for naturally occurring bioactive compounds, but their availability is limited due to geographical and seasonal variations. The elicitation approach can enhance the biosynthesis of phytochemicals during in vitro culture of G. sylvestre. Here, to further improve gymnemic acid II (GA II) and phenolic compounds (PC) production by G. sylvestre, cell suspension cultures (CSC), which has attracted attention for the production of essential phytochemicals, was explored using copper oxide nanoparticles (CuO NPs). Callus was obtained on MS medium containing 2,4-dichlorophenoxyacetic acid, kinetin, phytoagar, and sucrose. Agar-free MS medium was used to initiate CSC, which was treated with three concentrations of CuO NPs (1, 3 or 5 mg/L). Treatment for 48 h with 3 mg/L CuO NPs resulted in the greatest yields of GA II, total phenolics, and flavonoids. The cultures also displayed pronounced antioxidant, antidiabetic, anti-inflammatory, antibacterial, antifungal, and anticancer activities. The use of CuO NPs (3 mg/L) significantly increased the production of GA II (nine-fold) and PC compared to unamended CSC. We propose that CSC and use of nanoparticles (NPs) as a new generation of elicitors, offer a suitable prospect for the production of bioactive compounds.

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Section: Antidiabetic Activitymentioning

confidence: 91%

Section: Antidiabetic Activitymentioning

confidence: 99%

Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br

et al. 2019

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“…Mangiferin can stimulate β cell function via dipeptidyl peptidase-IV (DPP-4) inhibition [28]. Gymnemic acid has been proposed to protect β cells against oxidative damage and stimulate glucose-induced insulin secretion by recruiting glucose transporter type (GLUT)2 signalling [29]. Stevioside can directly target pancreatic β cells and promote insulin secretion [30].…”

Section: Plant-derived Small Molecules As Antidiabetic Agentsmentioning

confidence: 99%

Plant-Based Antidiabetic Nanoformulations: The Emerging Paradigm for Effective Therapy

Dewanjee

Chakraborty

Mukherjee

et al. 2020

IJMS

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Diabetes mellitus is a life-threatening metabolic syndrome. Over the past few decades, the incidence of diabetes has climbed exponentially. Several therapeutic approaches have been undertaken, but the occurrence and risk still remain unabated. Several plant-derived small molecules have been proposed to be effective against diabetes and associated vascular complications via acting on several therapeutic targets. In addition, the biocompatibility of these phytochemicals increasingly enhances the interest of exploiting them as therapeutic negotiators. However, poor pharmacokinetic and biopharmaceutical attributes of these phytochemicals largely restrict their clinical usefulness as therapeutic agents. Several pharmaceutical attempts have been undertaken to enhance their compliance and therapeutic efficacy. In this regard, the application of nanotechnology has been proven to be the best approach to improve the compliance and clinical efficacy by overturning the pharmacokinetic and biopharmaceutical obstacles associated with the plant-derived antidiabetic agents. This review gives a comprehensive and up-to-date overview of the nanoformulations of phytochemicals in the management of diabetes and associated complications. The effects of nanosizing on pharmacokinetic, biopharmaceutical and therapeutic profiles of plant-derived small molecules, such as curcumin, resveratrol, naringenin, quercetin, apigenin, baicalin, luteolin, rosmarinic acid, berberine, gymnemic acid, emodin, scutellarin, catechins, thymoquinone, ferulic acid, stevioside, and others have been discussed comprehensively in this review.

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“…GA obtained from G. sylvestre not only inhibits glucose absorption in the small intestine but also suppresses hyperglycemia and hyperinsulinemia in an oral glucose tolerance test. The efficiency of GA in inhibiting glucose absorption in the small intestine was found to be increased by a combined effect with acarbose and voglibose [49]. GA and PC have potent inhibitory effects on α-amylase and α-glucosidase [49,50].…”

Section: Antidiabetic Activitymentioning

confidence: 98%

Impact of Copper Oxide Nanoparticles on Enhancement of Gymnemic Acid and Phenolic Compounds Using Cell Suspension Culture of Gymnema sylvestre (Retz.) R. Br

Chung¹,

Thiruvengadam²

2019

Preprint

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Gymnema sylvestre is a pharmacological plant which has a rich source of bioactive compounds specifically gymnemic acid (GA) and phenolic compounds (PC) that used for pharmaceutical industries. Sources for naturally occurring bioactive compounds are limited, due to geographical and seasonal variations; on the other hand, it is commercially in demand. Biosynthesis of G. sylvestre phytochemicals through in vitro culture often enhanced by elicitation. The use of cell suspension cultures (CSC) has interested serious attention on the production of essential phytochemicals. The current study is aimed at improving the contents of GA and PC in G. sylvestre CSC using the copper oxide nanoparticles (CuO NPs). Callus was obtained on MS medium with 2.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), 0.1 mg/L kinetin (KIN), phytoagar (8.0 g/L), and sucrose (30 g/L). The above medium devoid of agar was used for the initiation of CSC. The CSC was treated with three levels of CuO NPs (1, 3 or 5 mg/L) to enhance the production of GA and PC. The greatest amount of GA (89.25 mg/g dry cell mass, DCM), total phenolic (245.10 mg/g), and flavonoid (4.57 mg/g) in CSC were achieved when G. sylvestre cells were treated for 48 h with 3 mg/L CuO NPs. Also, the biomedical potential (antioxidant, antidiabetic, anti-inflammatory, antibacterial, antifungal and anticancer activities) were also high in the CuO NPs (3 mg/L) treated CSC extracts of G. sylvestre. CuO NPs elicitation of CSC significantly increased production of GA (9-fold), and PC than non-elicited CSC in G. sylvestre.

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In Vitro Antidiabetic Effects of Isolated Triterpene Glycoside Fraction from Gymnema sylvestre

Cited by 29 publications

References 34 publications

Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br

Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br

Plant-Based Antidiabetic Nanoformulations: The Emerging Paradigm for Effective Therapy

Impact of Copper Oxide Nanoparticles on Enhancement of Gymnemic Acid and Phenolic Compounds Using Cell Suspension Culture of <em>Gymnema sylvestre</em> (Retz.) R. Br

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