<i>In vitro</i>antifungal activity of terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol against mycotoxigenic plant pathogens

Morcia, Caterina; Malnati, Mauro S.; Terzi, Valeria

doi:10.1080/19440049.2011.643458

Cited by 119 publications

(127 citation statements)

References 35 publications

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“…RC of each source of variation showed that the oil concentration is the main factor affecting model variation. Our findings are in harmony with the obtained results by Morcia et al (2012). When 1, 5, and 10 % aqueous emulsions of clove extracts were applied for control Fusarium wilt disease in muskmelon, the 10 % formulation caused stunting and other phytotoxic symptoms (Bowers and Locke 2000).…”

Section: Discussionsupporting

confidence: 79%

“…The highest antifungal activity of this essential oil was observed against DQ086833 (zone of inhibition of 5 cm mm at 2 % concentration) followed by AY264267 with zone of inhibition of 4.5 cm. The phenolic component of eugenol showed high level of toxicity against plant pathogenic fungi (Morcia et al 2012;Matan et al 2014). There are several possibilities for antifungal mechanism of EOs described by researchers but the exact mechanism has not been verified.…”

Section: Discussionmentioning

confidence: 99%

“…10 Effectiveness of EON in controlling Fusarium wilt under greenhouse conditions. a Cotton seedlings treated with eugenol oil nanoemulsion b, Severely affected seedlings after planting in artificially infested soil in a greenhouse test (untreated cottonseed) (Vein discoloration began at the margin of cotyledonary leaf) a Relative contribution (RC) to variation in radicle lengths calculated as percentage of sum of square of the explained (model) variation of food-borne (Ghosh et al 2014), of plant pathogens (Wang et al 2010), and mycotoxigenic fungi (Morcia et al 2012). On the other hand, little attention has been paid to its antifungal activity against Fusarium wilt in cotton.…”

Section: Discussionmentioning

confidence: 99%

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Eugenol oil nanoemulsion: antifungal activity against Fusarium oxysporum f. sp. vasinfectum and phytotoxicity on cottonseeds

2015

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The current research deals with the formulation and characterization of bio-based oil-in-water nanoemulsion. The formulated eugenol oil nanoemulsion was characterized by dynamic light scattering, stability test, transmission electron microscopy and thin layer chromatography. The nanoemulsion droplets were found to have a Z-average diameter of 80 nm and TEM study reveals the spherical shape of eugenol oil nanoemulsion (EON). The size of the nanoemulsion was found to be physically stable up to more than 1-month when it was kept at room temperature (25°C). The TEM micrograph showed that the EON was spherical in shape and moderately mono or di-dispersed and was in the range of 50-110 nm. Three concentrations of the nanoformulation were used to evalute the anti-fusarium activity both in vitro and in vivo experiments. SDS-PAGE results of total protein from the Fusarium oxysporum f. sp. vasinfectum (FOV) isolate before and after treatment with eugenol oil nanoemulsion indicate that the content of extra cellular soluble small molecular proteins decreased significantly in EON-treated fungus. Light micrographs of mycelia and spores treated with EON showed the disruption of the fungal structures. The analysis of variance (ANOVA) for Fusarium wilt incidence indicated highly significant (p = 0.000) effects of concentration, genotype, and their interaction. The difference in wilt incidence between concentrations and control was not the same for each genotype, that is, the genotypes responded differently to concentrations. Effects of three EON concentration on germination percentage, and radicle length, were determined in the laboratory. One very interesting finding in the current study is that cotton genotypes was the most important factors in determining wilt incidence as it accounted for 93.18 % of the explained (model) variation. In vitro experiments were conducted to evaluate the potential phytotoxic effect of three EON concentrations. Concentration, genotype and concentration x genotype interaction were all highly significant sources of variation in seed germination; however, interaction was the first in importance as a source of variation followed by the concentration, while genotype was the least important source of variation. These results suggest the potential use of eugenol oil nanoemulsion for protecting seedcotton from Fusarium wilt infection.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Eugenol oil nanoemulsion: antifungal activity against Fusarium oxysporum f. sp. vasinfectum and phytotoxicity on cottonseeds

2015

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“…Numbers and letters in bold type indicate statistically significant differences (REML, fixed = treatment, random = genotype, paired Tukey's post-hoc test, P<0.05) and marginally significant differences (P<0.10, in italics) (Bridges 1987;De Alwis et al 2009;Espinosa-garcia and Langenheim 1991;Kohzaki et al 2009;Paine and Hanlon 1994). Herbicide application also can increase the concentration of terpinen-4-ol in P. ponderosa (Kidd and Reid 1979), a compound with remarkable biological activity on fungi (Kusumoto et al 2014;Morcia et al 2012;Nenoff et al 1996) and bacteria (Kotan et al 2007). Manool concentrations can increase in conifers under biotic attack (Hanari et al 2002;Tomlin et al 2000), and can inhibit growth of several canker agents (Yamamoto et al 1997) and pathogenic bacteria (Ulubelen et al 1994).…”

Section: Discussionmentioning

confidence: 99%

Strong Induction of Minor Terpenes in Italian Cypress, Cupressus sempervirens, in Response to Infection by the Fungus Seiridium cardinale

Achotegui-Castells

Danti²,

Llusià

et al. 2015

J Chem Ecol

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Seiridium cardinale, the main fungal pathogen responsible for cypress bark canker, is the largest threat to cypresses worldwide. The terpene response of canker-resistant clones of Italian cypress, Cupressus sempervirens, to two differently aggressive isolates of S. cardinale was studied. Phloem terpene concentrations, foliar terpene concentrations, as well as foliar terpene emission rates were analyzed 1, 10, 30, and 90 days after artificial inoculation with fungal isolates. The phloem surrounding the inoculation point exhibited de novo production of four oxygenated monoterpenes and two unidentified terpenes. The concentrations of several constitutive mono-and diterpenes increased strongly (especially α-thujene, sabinene, terpinolene, terpinen-4-ol, oxygenated monoterpenes, manool, and two unidentified diterpenes) as the infection progressed. The proportion of minor terpenes in the infected cypresses increased markedly from the first day after inoculation (from 10 % in the control to 30-50 % in the infected treatments). Foliar concentrations showed no clear trend, but emission rates peaked at day 10 in infected trees, with higher δ-3-carene (15-fold) and total monoterpene (10-fold) emissions than the control. No substantial differences were found among cypresses infected by the two fungal isolates. These results suggest that cypresses activate several direct and indirect chemical defense mechanisms after infection by S. cardinale.

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“…CIN is one of the most valuable and important monoterpenes because its antimicrobial (Van Vuuren and Viljoen, 2007) and antifungal (Morcia et al, 2012) effects. There are many reports on antimicrobial effects of both leaves of L. nobilis and 1,8-cineole (Van Vuuren and Viljoen, 2007).…”

Section: Introductionmentioning

confidence: 99%

Priming effects of leaves of Laurus nobilis L. and 1,8-cineole on carbonmineralization

Koçak

Darici

2016

Chilean J. Agric. Res.

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In vitroantifungal activity of terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol against mycotoxigenic plant pathogens

Cited by 119 publications

References 35 publications

Eugenol oil nanoemulsion: antifungal activity against Fusarium oxysporum f. sp. vasinfectum and phytotoxicity on cottonseeds

Eugenol oil nanoemulsion: antifungal activity against Fusarium oxysporum f. sp. vasinfectum and phytotoxicity on cottonseeds

Strong Induction of Minor Terpenes in Italian Cypress, Cupressus sempervirens, in Response to Infection by the Fungus Seiridium cardinale

Priming effects of leaves of Laurus nobilis L. and 1,8-cineole on carbonmineralization

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