<i>In Vitro</i> Antifungal Activity of Silver Nanoparticles Against Ocular Pathogenic Filamentous Fungi

Xu, Yan; Gao, Chuanwen; Li, Xiaohua; He, Yihong; Zhou, Le; Pang, Guangren; Sun, Shiping

doi:10.1089/jop.2012.0155

Cited by 80 publications

(39 citation statements)

References 27 publications

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“…Corneal collagen cross-linking (CXL) is a relatively new technique currently been investigated in conjunction with photo-activated riboflavin (PAR) in refractory keratitis cases [102,137]. Investigations into the use of nanoparticle technology with terbinafine and silver have also shown promising results [189,214]. In addition, topical aqueous garlic extracts have been tested on rabbit keratitis models infected with A. flavus [76].…”

Section: Treatmentmentioning

confidence: 99%

Pathogenesis of microbial keratitis

Lakhundi

Siddiqui

Khan

2017

Microbial Pathogenesis

181

121

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Microbial keratitis is a sight-threatening ocular infection caused by bacteria, fungi, and protist pathogens. Epithelial defects and injuries are key predisposing factors making the eye susceptible to corneal pathogens. Among bacterial pathogens, the most common agents responsible for keratitis include Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumonia and Serratia species. Fungal agents of corneal infections include both filamentous as well as yeast, including Fusarium, Aspergillus, Phaeohyphomycetes, Curvularia, Paecilomyces, Scedosporium and Candida species, while in protists, Acanthamoeba spp. are responsible for causing ocular disease. Clinical features include redness, pain, tearing, blur vision and inflammation but symptoms vary depending on the causative agent. The underlying molecular mechanisms associated with microbial pathogenesis include virulence factors as well as the host factors that aid in the progression of keratitis, resulting in damage to the ocular tissue. The treatment therefore should focus not only on the elimination of the culprit but also on the neutralization of virulence factors to minimize the damage, in addition to repairing the damaged tissue. A complete understanding of the pathogenesis of microbial keratitis will lead to the rational development of therapeutic interventions. This is a timely review of our current understanding of the advances made in this field in a comprehensible manner. Coupled with the recently available genome sequence information and high throughput genomics technology, and the availability of innovative approaches, this will stimulate interest in this field.

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Section: Treatmentmentioning

confidence: 99%

Pathogenesis of microbial keratitis

Lakhundi

Siddiqui

Khan

2017

Microbial Pathogenesis

181

121

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“…Results demonstrated that AgNPs had higher activity as compared to natamycin. 258 Similarly, Dar et al studied the biocidal potential of AgNPs synthesized from Cryphonectria spp. against S. typhi, E. coli, S. aureus, and C. albicans, concluding that AgNPs can be used as potential antifungal agents.…”

mentioning

confidence: 99%

Nanosilver: new ageless and versatile biomedical therapeutic scaffold

Khan¹,

Saleh²,

Wahab³

et al. 2018

IJN

170

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Silver nanotechnology has received tremendous attention in recent years, owing to its wide range of applications in various fields and its intrinsic therapeutic properties. In this review, an attempt is made to critically evaluate the chemical, physical, and biological synthesis of silver nanoparticles (AgNPs) as well as their efficacy in the field of theranostics including microbiology and parasitology. Moreover, an outlook is also provided regarding the performance of AgNPs against different biological systems such as bacteria, fungi, viruses, and parasites (leishmanial and malarial parasites) in curing certain fatal human diseases, with a special focus on cancer. The mechanism of action of AgNPs in different biological systems still remains enigmatic. Here, due to limited available literature, we only focused on AgNPs mechanism in biological systems including human (wound healing and apoptosis), bacteria, and viruses which may open new windows for future research to ensure the versatile application of AgNPs in cosmetics, electronics, and medical fields.

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“…Investigations into the use of nanoparticle technology with terbinafine and silver have shown promise [66,67]. There has been an attempt to develop an antimicrobial contact lens incorporating melimine into the lens substance [68].…”

Section: Methodsmentioning

confidence: 99%

Fungal Keratitis: Update for 2014

Stone

Tan

2014

Curr Ophthalmol Rep

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Fungal keratitis is a challenging problem worldwide, and many obstacles remain to the effective prevention, diagnosis, and therapy of this vision-threatening condition. Recent research has highlighted progress in each of these facets of the clinical approach to keratomycosis. Increased awareness of contact lens-associated fungal keratitis has prompted regulators to pay greater attention to testing of contact lens solutions under realworld conditions. Related research has also delved into the mechanisms of fungal biofilm formation and related therapeutic targets. Advances in polymerase chain reaction methodologies promise to increase the rapidity and accuracy of diagnosis, thereby decreasing the delay in instituting appropriate therapy. Antifungal pharmacologic agents remain the first line of therapy, with the newer azole antifungal voriconazole being utilized in innovative ways, such as via intrastromal injection. Large prospective studies such as the Mycotic Ulcer Treatment Trial have helped delineate optimal treatment algorithms, and variations in surgical techniques and postoperative regimens continue to be refined. Natamycin may still be more effective than other recently utilized antifungals for the treatment of Fusarium infections, but treatment failure is still a concern. Post-keratoplasty immunosuppression may benefit from the use of topical calcineurin inhibitors such as cyclosporine and tacrolimus, given their intrinsic antifungal properties. It can be argued that the prevention, diagnosis, and treatment of fungal keratitis lag behind the state of the art for bacterial keratitis, but dramatic improvements are undoubtedly on the horizon.

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In Vitro Antifungal Activity of Silver Nanoparticles Against Ocular Pathogenic Filamentous Fungi

Abstract: Nano-Ag, relative to natamycin, exhibits potent in vitro activity against ocular pathogenic filamentous fungi.

Cited by 80 publications

References 27 publications

Pathogenesis of microbial keratitis

Pathogenesis of microbial keratitis

Nanosilver: new ageless and versatile biomedical therapeutic scaffold

Fungal Keratitis: Update for 2014

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