<i>In vitro</i> antigonococcal activity of urogenital coagulase-negative staphylococci

Lafond, L; Bisaillon, Jean‐Guy; Saheb, Samir A.; Beaudet, Réjean; Sylvestre, Michel

doi:10.1139/m81-021

Cited by 4 publications

(7 citation statements)

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“…Bacterial strains. S. haemolyticus 7 and 62 and S. epidermidis 56 were isolated from urogenital flora (3,18) and used for production of the respective antigonococcal and hemolytic substances H7, H62, and E56. Neisseria gonorrhoeae G-10 was the reference target strain (3), and it was cultivated as previously described (9).…”

Section: Methodsmentioning

confidence: 99%

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Comparative study of hemolytic substances produced by coagulase-negative Staphylococcus strains

1990

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Hemolytic substances H7, H62, and E56, produced by Staphylococcus haemolyticus 7 and 62 and S. epidermidis 56, respectively, were purified. H7 and H62 are probably similar on the basis of their isoelectric focusing profiles in 8 M urea and complete immunological identity as revealed by immunodiffusion with rabbit anti-H7 and anti-E56 sera. For E56, we observed seven bands instead of three in isoelectric focusing and only partial immunological identity with H7 and H62. However, H7 and E56 were similar with regard to the following characteristics: hemolytic spectra against different erythrocytes, kinetics of erythrocyte lysis, heat stability, and inhibition by phosphatidylcholine. E56 was not active at a temperature lower than or equal to 25°C, and its activity increased more rapidly with increased temperature compared with H7. For both substances, the complexes obtained by molecular filtration on Ultrogel AcA54 and the purified peptides by reverse-phase high-pressure liquid chromatography showed some hemolytic activity. These results suggest that a particular association or the presence of a given peptide could enhance the activity.

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Section: Methodsmentioning

confidence: 99%

“…The protein components were separated from the lipids by chromatography on Ultrogel AcA54 in 4 M urea. Purification of the different substances was monitored by quantitative determination of the antigonococcal activities of the different preparations as already described (18).…”

Section: Methodsmentioning

confidence: 99%

Comparative study of hemolytic substances produced by coagulase-negative Staphylococcus strains

1990

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“…7 was used for the production of the bactericidal inhibitor (Bisaillon et al, 1980;Lafond et al, 1981;Beaudet et al, 1982). The reference target strain was Neisseria gonorrhoeae G-10 and it was cultivated as previously described (Frenette et al, 1984).…”

Section: Materials and Methods Bacterial Strainsmentioning

confidence: 99%

The amino acid sequence of a gonococcal growth inhibitor from Staphylococcus haemolyticus

Watson

Yaguchi

Bisaillon

et al. 1988

Biochemical Journal

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A gonococcal inhibitor produced by Staphylococcus haemolyticus was separated into three components by reverse-phase h.p.l.c. The amino acid composition analysis of each of the three components indicated extensive similarities. N-Terminal sequence analysis of all three components allowed the identification of the first 27-30 residues of each. The complete primary structure of each component was determined from the sequence analysis of trypic peptides and peptides generated by mild acid hydrolysis. Each component is composed of 44 amino acid residues, with evidence suggesting the presence of an N-terminal formylmethionine residue in each. The components I, II and III have respectively 33, 29 and 33 identical amino acid residues in their sequences, which represents 75 %, 65.9 % and 75 % homology. These components contain a high proportion of hydrophobic amino acids, and their hydrophobicity profiles are closely related. Also, each of the three components contains a positively charged residue (lysine) as the third residue, followed by a core of hydrophobic residues. These results suggest that the three components are possible signal sequences of one or more secreted or membrane-associated proteins.

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“…7 of our collection and was selected from the urogenital flora (2) for its broad antigonococcal spectrum and narrow antibacterial spectrum. It was chosen mainly because it has been previously shown to produce an antigonococcal activity on semisolid agar cultures that can be recovered in the supernatant after centrifugation (18). The target strain, N. gonorrhoeae G-10, was previously described (2,18).…”

Section: Methodsmentioning

confidence: 99%

“…The crude supernatant was obtained by centrifuging frozen and thawed semisolid brain heart infusion (BHI) medium (GIBCO) which had supported a 24-h growth of staphylococcus no. 7 strain at 37°C as previously described (18). Supernatants from uninoculated semisolid media were used as controls.…”

Section: Methodsmentioning

confidence: 99%

Production, purification, and preliminary characterization of a gonococcal growth inhibitor produced by a coagulase-negative staphylococcus isolated from the urogenital flora

Beaudet¹,

Bisaillon²,

Saheb³

et al. 1982

Antimicrob Agents Chemother

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Coagulase-negative staphylococcus no. 7, isolated from the urogenital flora, was grown on semisolid brain heart infusion medium. Supernatants were obtained by centrifuging frozen and thawed media which had supported the growth of the staphylococci at 37 degrees C. The kinetic of production revealed that the antigonococcal activity was detected at the end of the logarithmic phase of growth and that the maximum activity was obtained after 24 h of incubation. Production of inhibitory activity was detected in cultures grown between 35 and 39 degrees C and in a pH range of 6.9 to 9.4. The inhibitory substance was purified by methanol extraction, acetone fractionation, dialysis, and chromatography on Ultrogel AcA 54. The characterization of the inhibitor showed that it was a lipoprotein or a lipid-associated-protein and that the protein component could be separated from the lipids when chromatographed on Ultrogel AcA 54 in the presence of urea. The inhibitory activity was associated with the protein component which had a molecular weight of approximately 15,900. In polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and urea, the protein component was dissociated in a subunit estimated to be approximately 1,400 daltons.

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In vitro antigonococcal activity of urogenital coagulase-negative staphylococci

Cited by 4 publications

References 0 publications

Comparative study of hemolytic substances produced by coagulase-negative Staphylococcus strains

Comparative study of hemolytic substances produced by coagulase-negative Staphylococcus strains

The amino acid sequence of a gonococcal growth inhibitor from Staphylococcus haemolyticus

Production, purification, and preliminary characterization of a gonococcal growth inhibitor produced by a coagulase-negative staphylococcus isolated from the urogenital flora

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