In vitroantimicrobial effects and mechanism of atmospheric-pressure He/O2plasma jet onStaphylococcus aureusbiofilm

Xu, Zimu; Shen, Jie; Cheng, Cheng; Hu, Shuheng; Liu, Yan; Chu, Paul K.

doi:10.1088/1361-6463/aa593f

Cited by 54 publications

(33 citation statements)

References 67 publications

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“…The VBNC state occurs when bacteria face environmental stress such as oxidative stress, desiccation, starvation, osmotic stress, and other adverse conditions [38]. It has been reported that non-thermal plasmas also convert micro-organisms into the VBNC state [8,39,40], acting as an environmental stress. Since the RS in the liquid inhibit bacterial growth, RS such as OH − , H 2 O 2 , NO , 3 and O 3 can be classified as environmental stress.…”

Section: Metabolic Activity Of Bacteria After the Plasma Treatmentmentioning

confidence: 99%

Control of multidrug-resistant planktonicAcinetobacter baumannii: biocidal efficacy study by atmospheric-pressure air plasma

Ruan

Guo

Gao

et al. 2018

Plasma Sci. Technol.

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In this research, an atmospheric-pressure air plasma is used to inactivate the multidrug-resistant Acinetobacter baumannii in liquid. The efficacy of the air plasma on bacterial deactivation and the cytobiological variations after the plasma treatment are investigated. According to colony forming units, nearly all the bacteria (6-log) are inactivated after 10 min of air plasma treatment. However, 7% of the bacteria enter a viable but non-culturable state detected by the resazurin based assay during the same period of plasma exposure. Meanwhile, 86% of the bacteria lose their membrane integrity in the light of SYTO 9/PI staining assay. The morphological changes in the cells are examined by scanning electron microscopy and bacteria with morphological changes are rare after plasma exposure in the liquid. The concentrations of the long-living RS, such as H 2 O 2 , NO , 3 and O 3 , in liquid induced by plasma treatment are measured, and they increase with plasma treatment time. The changes of the intracellular ROS may be related to cell death, which may be attributed to oxidative stress and other damage effects induced by RS plasma generated in liquid. The rapid and effective bacteria inactivation may stem from the RS in the liquid generated by plasma and air plasmas may become a valuable therapy in the treatment of infected wounds.

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Section: Metabolic Activity Of Bacteria After the Plasma Treatmentmentioning

confidence: 99%

Control of multidrug-resistant planktonicAcinetobacter baumannii: biocidal efficacy study by atmospheric-pressure air plasma

Ruan

Guo

Gao

et al. 2018

Plasma Sci. Technol.

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“…In this paper, a model tissue made of gelatin gel was used to investigate the spatial‐temporal distribution of ROS generated by a He + O 2 plasma jet. The volume fraction of O 2 was controlled to be 0.5% in the working gas, because such working gas was reportedly easy to generate diffuse plasma and effective to produce ROS . KI‐starch reagent was mixed with gelatin gel to indicate the ROS distribution in the model tissue by color‐forming reactions .…”

Section: Introductionmentioning

confidence: 99%

“…The volume fraction of O 2 was controlled to be 0.5% in the working gas, because such working gas was reportedly easy to generate diffuse plasma and effective to produce ROS. [16] KI-starch reagent was mixed with gelatin gel to indicate the ROS distribution in the model tissue by color-forming reactions. [17] The spatialtemporal distribution of ROS was obtained as a function of several important parameters including the gas flow rate, the plasma treatment time, the water content in model tissue, and the inclination angle of plasma irradiation.…”

Section: Introductionmentioning

confidence: 99%

Spatial‐temporal distributions of ROS in model tissues treated by a He+O₂ plasma jet

Liu

et al. 2018

Plasma Processes & Polymers

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The transportation of reactive oxygen species (ROS) to lesion site through human tissue is crucial for plasma medicine. For this reason, the spatial‐temporal distributions of ROS in model tissues irradiated by a He + O2 plasma jet were investigated in this paper. It was found that the ROS formed a ring‐shaped surface pattern on the model tissue, of which the diameter increased with the gas flow rate but remained unchanged with the plasma treatment time. The surface pattern changed significantly with the inclination angle of plasma irradiation, suggesting that the ROS dosage is difficult to precisely control for clinical applications. Moreover, the penetration depth of O3 increased linearly with the plasma treatment time or the water content of the model tissue.

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“…These oxygen species play a significant role in the sterilization process because these species have strong oxidative effects on the outer membrane of the bacterial cell [39,54,115]. The addition of oxygen in the working gas contributes to the deactivation efficacy of the plasma jet by the combined action of plasma-induced endogenous ROS and the plasma generated ROS as reveal by the study [116]. This combined effect damages the bacterial membrane leading to biofilm deactivation [116].…”

Section: Role Of the Gas Or The Gas Mixture With Its Flow Ratementioning

confidence: 99%

“…The addition of oxygen in the working gas contributes to the deactivation efficacy of the plasma jet by the combined action of plasma-induced endogenous ROS and the plasma generated ROS as reveal by the study [116]. This combined effect damages the bacterial membrane leading to biofilm deactivation [116]. Nitrogen has also been added to the noble gases to increase the formation of reactive nitrogen species [107].…”

Section: Role Of the Gas Or The Gas Mixture With Its Flow Ratementioning

confidence: 99%

Application of Non-Thermal Plasma on Biofilm: A Review

Gupta

Ayan

2019

Applied Sciences

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The formation of bacterial biofilm on implanted devices or damaged tissues leads to biomaterial-associated infections often resulting in life-threatening diseases and implant failure. It is a challenging process to eradicate biofilms as they are resistant to antimicrobial treatments. Conventional techniques, such as high heat and chemicals exposure, may not be suitable for biofilm removal in nosocomial settings. These techniques create surface degradation on the treated materials and lead to environmental pollution due to the use of toxic chemicals. A novel technique known as non-thermal plasma has a great potential to decontaminate or sterilize those nosocomial biofilms. This article aims to provide readers with an extensive review of non-thermal plasma and biofilms to facilitate further investigations. A brief introduction summarizes the problem caused by biofilms in hospital settings with current techniques used for biofilm inactivation followed by the literature review strategy. The remainder of the review discusses plasma and its generation, the role played by plasma reactive species, various factors affecting the antimicrobial efficacy of non-thermal plasma and summarizes many studies published in the field.

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In vitroantimicrobial effects and mechanism of atmospheric-pressure He/O₂plasma jet onStaphylococcus aureusbiofilm

Cited by 54 publications

References 67 publications

Control of multidrug-resistant planktonicAcinetobacter baumannii: biocidal efficacy study by atmospheric-pressure air plasma

Control of multidrug-resistant planktonicAcinetobacter baumannii: biocidal efficacy study by atmospheric-pressure air plasma

Spatial‐temporal distributions of ROS in model tissues treated by a He+O₂ plasma jet

Application of Non-Thermal Plasma on Biofilm: A Review

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In vitroantimicrobial effects and mechanism of atmospheric-pressure He/O2plasma jet onStaphylococcus aureusbiofilm

Cited by 54 publications

References 67 publications

Control of multidrug-resistant planktonicAcinetobacter baumannii: biocidal efficacy study by atmospheric-pressure air plasma

Control of multidrug-resistant planktonicAcinetobacter baumannii: biocidal efficacy study by atmospheric-pressure air plasma

Spatial‐temporal distributions of ROS in model tissues treated by a He+O2 plasma jet

Application of Non-Thermal Plasma on Biofilm: A Review

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In vitroantimicrobial effects and mechanism of atmospheric-pressure He/O₂plasma jet onStaphylococcus aureusbiofilm

Spatial‐temporal distributions of ROS in model tissues treated by a He+O₂ plasma jet