<i>In Vitro</i>Antimicrobial Susceptibility of 183<i>Pseudomonas aeruginosa</i>Strains Isolated from Dogs to Selected Antipseudomonal Agents

Šeol, Branka; Naglić, Tomo; Madić, Josip; Bedeković, Maja

doi:10.1046/j.1439-0450.2002.00548.x

Cited by 36 publications

(40 citation statements)

References 21 publications

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“…isolated from chronic otitis externa cases in dogs. The 83.1% of 183 P. aeruginosa strains were found susceptible to gentamicin [11]. In our study 89.7% of the isolates were susceptible to gentamicin.…”

Section: Discussionsupporting

confidence: 41%

“…isolated from dogs was proven [10]. 183 P. aeruginosa strains isolated from the outer ear canal of dogs were investigated and they found that 93.4% were sensitive to marbofl oxacin [11]. 26.1% resistance ratio was reported [1] in P. aeruginosa strains isolated from otitis cases in dogs and cats.…”

Section: Introductionmentioning

confidence: 99%

“…isolated from chronic otitis externa cases in dogs. 183 P. aeruginosa strains isolated from the outer ear canal of dogs [11] were investigated; 83.1% of the strains were susceptible to gentamicin. Similar results [13] were reported, 84.6% of the investigated 39 P. aeruginosa strains were sensitive to gentamicin.…”

Section: Introductionmentioning

confidence: 99%

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Evaluating synergy between marbofloxacin and gentamicin in Pseudomonas aeruginosa strains isolated from dogs with otitis externa

Jerzsele

Pásztiné-Gere

2015

Acta Microbiologica Et Immunologica Hungarica

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The aim of this study was to determine antimicrobial susceptibility of Pseudomonas aeruginosa strains to marbofl oxacin and gentamicin, and investigate the possible synergistic, additive, indifferent or antagonistic effects between the two agents. P. aeruginosa strains can develop resistance quickly against certain antibiotics if used alone, thus the need emerges to fi nd synergistic combinations. A total of 68 P. aeruginosa strains isolated from dogs were examined. In order to describe interactions between marbofl oxacin and gentamicin the checkerboard microdilution method was utilized. The MICs (minimum inhibitory concentrations) for marbofl oxacin and gentamicin were in the range 0.25-64 mg/L and 0.25-32 mg/L, respectively. The combination of marbofl oxacin and gentamicin was more effective with a MIC range of 0.031-8 mg/L and a MIC 90 of 1 mg/L, compared to 16 mg/L for marbofl oxacin alone and 8 mg/L for gentamicin alone. The FIC (fractional inhibitory concentration) indices ranged from 0.0945 (pronounced synergy) to 1.0625 (indifference). Synergy between marbofl oxacin and gentamicin was found in 33 isolates. The mean FIC index is 0.546, which represents a partial synergistic/additive effect close to the full synergy threshold. In vitro results indicate that marbofl oxacin and gentamicin as partially synergistic agents may prove clinically useful in combination therapy against P. aeruginosa infections. Although marbofl oxacin is not used in the human practice, the interactions between fl uoroquinolones and aminoglycosides may have importance outside the veterinary fi eld.

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Section: Discussionsupporting

confidence: 41%

Section: Introductionmentioning

confidence: 99%

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Evaluating synergy between marbofloxacin and gentamicin in Pseudomonas aeruginosa strains isolated from dogs with otitis externa

Jerzsele

Pásztiné-Gere

2015

Acta Microbiologica Et Immunologica Hungarica

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“…Spencer (1996) found that 95% of isolates were sensitive to ceftazidime, Panayotis et al (1998) reported 15% isolate resistance, Shawar et al (1999) 11.1%, Mueller-Premru and Gubina (2000) 9.1%, and De Champs et al (2002) 6%. In contrast, Seol et al (2002) found that 19.7% of P. aeruginosa isolated from canines was resistant to ceftazidime. The present study revealed that resistance to ceftazidime was 38.8% (17/49) for human isolates and 23.5% (4/17) for canine isolates.…”

Section: Resultsmentioning

confidence: 88%

Molecular mechanisms of ceftazidime resistance in Pseudomonas aeruginosa isolates from canine and human infections

Du¹,

Kuo²,

Cheng³

et al. 2010

Vet. Med. - Czech

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Sixty-six clinical P. aeruginosa isolates, 17 obtained from canine otitis specimens and 49 received from human patients with bloodstream infections, were collected between February 2007 and January 2008. The minimal inhibitory concentrations (MICs) of the antimicrobial agents of these isolates were determined. Multidrug resistance was common, with 23 (34.8%) isolates found to be ceftazidime resistant. To explore the mechanisms of ceftazidime resistance, PCR analyses were performed to detect drug-resistance genes. The prevalence rate of Ambler class A, B, and D β-lactamase genes were obtained, with bla TEM-1 100%, bla PSE-1 100%, bla OXA-2 96.2%, bla SHV-18 91.3%, bla OXA-17 78.3%, bla VIM-3 26.1%, bla OXA-10 21.7% and bla SHV-1 8.7%. An efflux inhibition assay with the PAβN compound was conducted. The ceftazidime resistance isolates were also tested by RT-qPCR to determine the mRNA expression levels of the oprM and ampC genes. Five (21.7%) of the ceftazidime resistance isolates appeared to overactivate the OprM efflux system. The ampD, ampE, and ampR genes and the ampC-ampR intergenic region were subsequently amplified and sequenced. Five (21.7%) of the ceftazidime resistance isolates from humans and canines had a point mutation in AmpR (Asp135-Asn, n = 3; Als194-Ser, n = 2), which induces AmpC overproduction from 10-to 80-fold. This study first reported ceftazidime resistance in P. aeruginosa from canine otitis specimens, which are closely related to ESBLs (50%), including the overproduction of AmpC (25%) and the OprM efflux system (25%). The ESBLs (100%) played an important role in all ceftazidime resistance isolates from humans, and either AmpC (21.1%) or OprM (21.1%) might be overexpressed within the same isolate. A human patient isolate (H307B) showed simultaneous expression of ESBLs, the OprM efflux system, and AmpC overproduction.

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“…Resistance to ciprofloxacin has not significantly changed since the first study was conducted in Croatia in 2002, when 93.4% of isolates were susceptible and 3.8% were resistant to ciprofloxacin (ŠEOL et al, 2002). The resistance rate reported in the Croatian study from 2011 (MEKIĆ et al, 2011) was higher (8.7%) but the main reason for that was the difference in the origin of the isolates.…”

Section: Discussionmentioning

confidence: 98%

Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs - comparing disk diffusion and microdilution methods

2017

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________________________________________________________________________________________ PINTARIĆ, S., K. MATANOVIĆ, B. ŠEOL MARTINEC:Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs -comparing disk diffusion and microdilution methods. Vet. arhiv 87, 291-300, 2017. ABSTRACTPseudomonas aeruginosa has been determined to be the distinct cause of a number of different infections in both humans and animals. Apart from its high intrinsic resistance, which makes it difficult to treat, it also has a great capacity to acquire further resistance mechanisms. These mechanisms can be present simultaneously in one cell, conferring a multiresistant phenotype. Taking into account that the known risk factor for selection of resistant strains is excessive and inappropriate drug use, especially fluoroquinolones, the aim of this study was to determine the susceptibility of P. aeruginosa isolates to these antibacterial agents. The susceptibility of 90 canine isolates was determined by disk diffusion susceptibility test and the broth microdilution method. Both methods showed that isolates were significantly more sensitive to ciprofloxacin than to marbofloxacin or enrofloxacin, and more sensitive to marbofloxacin than to enrofloxacin. The results of microdilution and disk diffusion testing were in 98.9% agreement for ciprofloxacin and in 91.1% agreement for marbofloxacin, with no statistically significant difference between the two methods (P>0.05) for these two antibiotics. For enrofloxacin, the results of microdilution and disk diffusion testing were in 76.6% agreement, with a statistically significant disagreement between the two methods (P<0.05). In 16 cases, the disk diffusion test overestimated the number of strains susceptible to enrofloxacin compared with the microdilution test. These results suggest that for P. aeruginosa infections in dogs, therapy using enrofloxacin, based on the results of disk diffusion testing, may lead to ineffective treatment and could contribute to selection of resistant and multiresistant strains.

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In VitroAntimicrobial Susceptibility of 183Pseudomonas aeruginosaStrains Isolated from Dogs to Selected Antipseudomonal Agents

Cited by 36 publications

References 21 publications

Evaluating synergy between marbofloxacin and gentamicin in Pseudomonas aeruginosa strains isolated from dogs with otitis externa

Evaluating synergy between marbofloxacin and gentamicin in Pseudomonas aeruginosa strains isolated from dogs with otitis externa

Molecular mechanisms of ceftazidime resistance in Pseudomonas aeruginosa isolates from canine and human infections

Fluoroquinolone susceptibility in Pseudomonas aeruginosa isolates from dogs - comparing disk diffusion and microdilution methods

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