In vitro Antioxidant Activity of Different Cultivars of Banana Flower (Musa paradicicus L.) Extracts Available in India

Abstract

Six different cultivars of banana flowers (Musa paradicicus) (Kathali, Bichi, Shingapuri, Kacha, Champa, and Kalabou) were analyzed for the content of polyphenol expressed as gallic acid equivalent and flavonoid expressed as quercetein equivalent, and the in vitro total antioxidative activities of the flower extracts were compared with standard and expressed as trolox equivalent. The reducing power, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS•(+)) scavenging activities, inhibition of lipid peroxidation in a linoleic acid emulsion system, and liposome peroxidation system were measured and compared with respective standard antioxidants. Iron-mediated Fenton reaction was carried out to evaluate the protective effect of the extract of banana flower (Kacha cultivar) against H(2)O(2)-induced DNA damage. The Kacha variety contains the maximum amount of polyphenol (11.94 ± 0.03 mg of gallic acid equivalent/g of dry weight) and flavonoid (0.174 ± 0.001 g of quercetin equivalent/g of polyphenol). It also has the highest total antioxidant capacity, DPPH radical scavenging activity, and ABTS•(+) radical scavenging activity with a least EC(50) value of 0.051 mg/mL. Hepatic cell damage in iron-mediated Fenton reaction caused by free radicals is reduced by the banana flower extract. On the basis of the results obtained, the banana flowers are found to be a potential source of natural antioxidants. This is the first report on the antioxidant properties of the extracts from banana flowers. The study suggests that the flowers of M. paradicicus that are found in India and consumed as vegetable can provide valuable functional ingredients that help in the prevention of oxidative stress.

Binding interaction study on human serum albumin with bactericidal gold nanoparticles synthesized from a leaf extract of Musa balbisiana: a multispectroscopic approach

“…). The observed functional groups are the constituent parts of polyphenols, flavonoids, anthocyanins, dopamines, serotonin in the MBLE . Considerable shifts in the peaks were detected at 3349, 3266, 1780, 1649, 1485, 1248 and 1081 cm −1 in the spectra after MGNs formation.…”

“…M. balbisiana is cultivated in more than 135 countries such as tropical Indomalaya, Australia, Papua New Guinea or India . Previously reported, aqueous M. balbisiana leaf extract (MBLE) contains polyphenols, flavonoids, minerals, anthocyanins, serotonin, dopamines . The M. balbisiana leaf is traditionally known as a natural antidote as it can reduce the effects of poisons from bee stings, spider bites, insect bites, plus rashes, skin irritation, minor skin wounds and in addition treat skin disorders such as dandruff, sunburn and eczema etc.…”

Polyphenolic profile and antioxidant properties of rhizome of commercial banana cultivars grown in India

“…Outer leaf sheaths of pseudostem of cultivars nendran, robusta and kathali exhibited total phenolics of 110, 94 and 79 mg GAE/g of extract, respectively and total flavonoids of 49, 38 and 27 mg QE/g of extract, respectively (Anusuya, Gomathi, Tharani, & Murugesan, 2013). Phenolic and flavonoid content of flower from six different banana cultivars ranged from 2.14 to 11.94 mg GAE and 0.023 to 0.174 mg QE/g of dry weight (China et al, 2011). In our earlier study, the polyphenolic content of pseudostem of eight different banana cultivars revealed that the TPC and TFC ranged from 7.58 to 291 mg GAE and 4 to 80 mg CE/g of extract (Saravanan & Aradhya, 2011b).…”

“…Acetone extract exhibited high reducing power with lowest EC 50 value of 6.5 mg/mL followed by methanol (21 mg/mL) and chloroform (90 mg/mL) ( Table 3). The activity was observed to be higher than the reducing power of juice from banana rhizome and pseudostem (Saravanan & Aradhya, 2011a), and also flower from different banana cultivars (China et al, 2011). The reducing power capacity of a BR extracts may serve as a significant indicator of antioxidant property by donates electrons to reactive free radicals species, thus promoting the termination of free radical chain reactions.…”

Anti-cancer potential of banana flower extract: An <i>in vitro</i> study

“…flower and pseudostem extracts. China et al, (2011) reported that, the banana flowers are a potential source of natural anti-oxidants.…”