<i>In vitro</i> antioxidant and antimicrobial activity of <i>Cannabis sativa</i> L. cv ʻFutura 75ʼ essential oil

Pellegrini, Marika; Palmieri, Sara; Ricci, Antonella; Serio, Annalisa; Paparella, Antonello; Sterzo, Claudio Lo

doi:10.1080/14786419.2020.1813139

Cited by 27 publications

(15 citation statements)

References 20 publications

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“…Later, in 2020, Martinenghi and coauthors 53 tested CBD against several G + pathogens, observing MIC values ranging from 3.18 to 6.36 µM. In addition, in 2020, Pellegrini and coauthors 54 tested the effect of hemp essential oil on S. aureus and found MIC values ranging from 3.97 to 15.90 µM. According to Wassmann and coauthors 55 , to date, little is known of the antimicrobial mechanism of action of cannabinoids; nevertheless, these authors explained that exposing S. aureus to CBD decreased the membrane potential.…”

Section: Resultsmentioning

confidence: 99%

Comparative assessment of antimicrobial, antiradical and cytotoxic activities of cannabidiol and its propyl analogue cannabidivarin

Russo

Lavorgna

Nugnes

et al. 2021

Sci Rep

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Cannabidiol and cannabidivarin are phytocannabinoids produced by Cannabis indica and Cannabis sativa. Cannabidiol has been studied more extensively than its propyl analogue cannabidivarin. Therefore, we performed a battery of in vitro biological assays to compare the cytotoxic, antiradical and antibacterial activities of both cannabinoids. Potential mitochondrial metabolism alterations, DNA synthesis inhibition, and plasma membrane damage were studied by MTT assay, BrdU-ELISA and LDH assay of cancer and normal human cells exposed to cannabinoids. ABTS and DPPH assays were performed to observe the effects of the cannabinoids on free radicals. Microbial susceptibility tests were performed to study the activity of the cannabinoids in two bacterial species implicated in human infections, Escherichia coli and Staphylococcus aureus. The results showed that the cannabinoids induced medium levels of cytotoxicity in cancer and normal cells at concentrations ranging from 15.80 to 48.63 and from 31.89 to 151.70 µM, respectively, after 72 h of exposure. Cannabinoids did not exhibit a strong antioxidant capacity in scavenging ABTS or DPPH radicals. No evident differences were observed between the two cannabinoids in antimicrobial activity, except with respect to S. aureus, which showed greater susceptibility to cannabidiol than to cannabidivarin after 72 h of exposure.

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Section: Resultsmentioning

confidence: 99%

Comparative assessment of antimicrobial, antiradical and cytotoxic activities of cannabidiol and its propyl analogue cannabidivarin

Russo

Lavorgna

Nugnes

et al. 2021

Sci Rep

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Section: Anti-microbial Activity Of Essential Oils From Cannabis Sati...mentioning

confidence: 99%

“…Pellegrini et al [69] observed that essential oil prepared from Cannabis sativa L. cultivar Futura 75 inflorescences with low ∆ 9 -THC content (<0.2%) cultivated in the Abruzzo territory showed anti-bacterial activity against Staphylococcus aureus and Listeria monocytogenes with a MIC of 1.25-5 µL/mL, while being ineffective against Salmonella enterica. They also showed that the essential oil possessed anti-oxidative properties [69]. The essential oils produced from the Cannabis sativa L. cultivar Futura 75 inflorescences was also found to have insecticidal activity with LD 50 values of 65.8 µg/larva on Spodoptera littoralis, 122.1 µg/adult on Musca domestica, and LC 50 of 124.5 µL/L on Culex quinquefasciatus larvae [70].…”

Section: Anti-microbial Activity Of Essential Oils From Cannabis Sati...mentioning

confidence: 99%

Anti-Microbial Activity of Phytocannabinoids and Endocannabinoids in the Light of Their Physiological and Pathophysiological Roles

Sionov

Steinberg

2022

Biomedicines

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Antibiotic resistance has become an increasing challenge in the treatment of various infectious diseases, especially those associated with biofilm formation on biotic and abiotic materials. There is an urgent need for new treatment protocols that can also target biofilm-embedded bacteria. Many secondary metabolites of plants possess anti-bacterial activities, and especially the phytocannabinoids of the Cannabis sativa L. varieties have reached a renaissance and attracted much attention for their anti-microbial and anti-biofilm activities at concentrations below the cytotoxic threshold on normal mammalian cells. Accordingly, many synthetic cannabinoids have been designed with the intention to increase the specificity and selectivity of the compounds. The structurally unrelated endocannabinoids have also been found to have anti-microbial and anti-biofilm activities. Recent data suggest for a mutual communication between the endocannabinoid system and the gut microbiota. The present review focuses on the anti-microbial activities of phytocannabinoids and endocannabinoids integrated with some selected issues of their many physiological and pharmacological activities.

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“…These findings indicate the susceptibility of the Gram-positive bacteria to the EO investigated, and are in accordance with many other studies, which have reported that Gram-positive bacteria are more susceptible to the inhibitory effects of essential oils than Gram-negative ones. 17 -19 According to the literature, the resistance of Gram-negative bacteria to essential oils has been attributed to the presence of a hydrophilic external lipopolysaccharide membrane that blocks the penetration of hydrophobic molecules of oil into the cells. 20 -22…”

Section: Resultsmentioning

confidence: 99%

Chemical Profiling, Antioxidant and Antibacterial Activities of Essential Oil From Englerastrum gracillimum Th. C. E. Fries Growing in Niger

Abba

Ilagouma

Amadou

et al. 2021

Natural Product Communications

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In the present study, the chemical composition, antioxidant and antibacterial activities of the essential oil obtained by hydrodistillation of the aerial parts of Englerastrum gracillimum Th. C. E. Fries growing in Niger were investigated. Gas chromatography-mass spectrometry (GC–MS) analysis of the essential oil resulted in the identification of 42 compounds representing 97.9% of the total oil constituents. The major compounds of the essential oil were: α-humulene (30.5%), followed by cubenol (19.8%), γ-muurolene (14.0%), ( E)-β-caryophyllene (5.8%), β-gurjunene (5.2%), and curzerene (4.9%). The antioxidant activity of the essential oil was determined by using the free radical-scavenging activity (2,2 diphenyl-1-picrylhydrazyl: DPPH˙) and ferric reducing antioxidant power assays. The essential oil showed good antioxidant potential with both methods. The antibacterial activity was evaluated against multi-resistant Acinetobacter baumannii P1483, extended-spectrum β-lactamase (ESBL) -Escherichia coli Bu8566, Salmonella spp. H1548, Proteus mirabilis Bu190 , Enterobacter cloacae Bu147, Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 700603), methicillin-resistant Staphylococcus aureus P1123, Enterococcus faecium H3434, and Staphylococcus aureus (ATCC 25923) using the agar disc diffusion and microdilution methods. The essential oil exhibited good antibacterial activity. The highest antibacterial effect was observed against Staphylococcus aureus ATCC 25923 and methicillin-resistant Staphylococcus aureus P1123 with minimal inhibitory concentration (MIC) values of 0.03 mg/mL and 0.06 mg/mL, respectively. These data are of scientific importance for the valorization of aromatic and medicinal plants of Niger, especially E. gracillimum. This study reports for the first time the antioxidant and antibacterial activities of this essential oil.

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In vitro antioxidant and antimicrobial activity of Cannabis sativa L. cv ʻFutura 75ʼ essential oil

Cited by 27 publications

References 20 publications

Comparative assessment of antimicrobial, antiradical and cytotoxic activities of cannabidiol and its propyl analogue cannabidivarin

Comparative assessment of antimicrobial, antiradical and cytotoxic activities of cannabidiol and its propyl analogue cannabidivarin

Anti-Microbial Activity of Phytocannabinoids and Endocannabinoids in the Light of Their Physiological and Pathophysiological Roles

Chemical Profiling, Antioxidant and Antibacterial Activities of Essential Oil From Englerastrum gracillimum Th. C. E. Fries Growing in Niger

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