In vitro antitumor activity in breast cancer cells (SKBR3 and PMC42) of Ruta graveolens in homeopathic dilution

Abstract

Objectives: Given the need to establish less invasive treatments that improve the patient’s quality of life and still have antitumour effects, this study aimed to evaluate the behaviour of breast cancer tumour cells (SKBR3 and PMC42) by treatment with ultradiluted Ruta graveolens (Ruta D35). Material and Methods: The breast cancer cell lines SKBR3 and PMC42 were treated with ultradiluted Ruta D35 and the in vitro effects on cell viability was evaluated by MTT cytotoxicity assay. Results: In the SKBR3 cells, Ruta D35 at concentration of 8 μl/ml induced a significant reduction in cell viability when compared to the untreated control. The treatment with Ruta D35 in the PMC42 cells reduced cell viability from the treatment at the highest concentrations of 20 μl/ml, 30 μl/ml, 40 μl/ml and 50 μl/ml. Conclusion: Ruta D35 presented in vitro cytotoxic activity in SKBR3 and PMC42 breast cancer cells, thereby reducing cell viability. However, further tests are necessary to better understand the cytotoxic action and the potential of R. graveolens for treating breast tumour cells.