In vitroassessment of molybdenum-induced secretory activity, proliferation and apoptosis of porcine ovarian granulosa cells

Abstract

Molybdenum (Mo) is an essential trace element and it plays an important role in cell functions. The mechanism of the action of molybdenum in connection with growth factor IGF-I, proliferation-related peptide cyclin B1 and apoptosis-related peptide caspase-3 has not been examined previously in porcine ovarian granulosa cells. The general objective of this in vitro study was to examine the secretory activity of porcine ovarian granulosa cells after experimental Mo administration and to outline the potential intracellular mediators of its effects. Ovarian granulosa cells were incubated with ammonium molybdate for 18 hours: 1.0 mg/mL; 0.5 mg/mL; 0.33 mg/mL; 0.17 mg/mL and 0.09 mg/mL, while the control group received no Mo. The secretion of IGF-I was assessed by RIA and expression of cyclin B1 and caspase-3 by immunocytochemistry. IGF-I release was decreased by Mo addition at the doses 1.0 mg/mL and 0.5 mg/mL. The expression of cyclin B1 was stimulated by Mo addition at all doses ranging from 1.0-0.09 mg/mL. Caspase-3 expression was also stimulated after experimental Mo addition at the doses 1.0 and 0.5 mg/mL. These data contribute to new insights regarding the mechanism of action of Mo on porcine ovarian functions, secretory activity, proliferation and apoptosis of granulosa cells through hormonal and intracellular substances such as are cyclin B1 and caspase-3.

Methoxychlor induces atresia by altering Bcl2 factors and inducing caspase activity in mouse ovarian antral follicles in vitro

“…While, to our knowledge, no studies have examined the effects of MXC on CASP activities in the ovary, some studies have shown that other chemicals induce CASP3 activity or protein levels in the ovarian cells. Studies in granulosa cells have shown that acute exposure to molybdenum and chromium induce apoptosis by inducing CASP3 [25, 31]. Other studies have shown that exposure to VCD induces apoptosis in primordial/primary follicles by inducing CASP3 [32, 33].…”

Cell apoptosis of caprine spleen induced by toxicity of cadmium with different levels of molybdenum

“…Furthermore, the caspase-3 pathway is involved in Cd-induced apoptosis in cortical neurons (Lopez et al, 2003). Wang et al (2013) demonstrated that the exposure of mice to 8 mg kg −1 CdCl 2 for 5 weeks increased the production of ROS and altered the levels of oxidative stress related biomarkers in the kidney, and induced apoptosis via mitochondria pathway by the activation of Caspase-3, and Caspase-3 was also stimulated after experimental Mo addition at the doses 1.0 and 0.5 mg mL −1 to ovarian granulosa cells (Kolesarova et al, 2011). In present study, the toxicity of cadmium with different levels of molybdenum significantly inhibited expression of Bcl-2 and up-regulated expression of Bax, Cyt c and Caspase-3, which is in compliance with previous studies (Liu et al, 2014;Zhou et al, 2013).…”

Novel Insights on the Corpus Luteum Function: Role of Vaspin on Porcine Luteal Cell Angiogenesis, Proliferation and Apoptosis by Activation of GRP78 Receptor and MAP3/1 Kinase Pathways

“…Furthermore, proliferation is closely related to cell cycle progression, which is regulated by cyclins [38]. Cyclin A is a well-established marker of porcine cell proliferation, growth, and development, and which level research as a peak in the S phase of the cell cycle [39]. Our results is in a good agreement with data obtained in porcine granulosa, where vaspin decreased apoptosis and caspase 3 level, while increased BAX/BCL2, cyclin A expression, and proliferation [24].…”