In vitro biocompatibility evaluation of radiolytically synthesized silver/polyvinyl hydrogel nanocomposites for wound dressing applications

Abstract

Nano silver/polyvinyl alcohol hydrogel nanocomposites have been prepared using gamma irradiation technique. Gamma irradiation serves as a crosslinking agent for the polyvinyl alcohol hydrogels and also acts as a reducing agent for reduction of Ag+ ions to zero valent Ag0 within the polyvinyl alcohol crosslinked network. The microstructural characteristics of the prepared composites were studied using powder X-ray diffraction, ultraviolet–visible spectroscopy, Fourier-transform infrared spectroscopy and field emission scanning electron microscopy analysis. The data obtained by these characterizations indicate the homogeneous distribution of silver nanoparticles on the polyvinyl alcohol network. The swelling properties and mechanical parameters of the silver/polyvinyl alcohol hydrogel nanocomposites tend to show improvements, making them a better material for wound care applications. The silver/polyvinyl alcohol hydrogel nanocomposites have shown good antibacterial potential against both Gram-positive and Gram-negative bacteria and shown nil or minimal cytotoxic effect on human melanoma (SK-MEL-2) and mouse melanoma (B16-F1) cell lines. Overall, it was concluded that under optimized condition, silver/polyvinyl alcohol hydrogel nanocomposites synthesized using gamma irradiation technique are excellent candidates for wound dressing application.

pH Responsive Properties of Methyl Red/PVA Polymers for Smart Food Packaging Applications

“…To study the way molecules are arranged in the MR/PVA polymer composites, the surface morphology of the Pure PVA, MRPVA3, and MRPVA3 treated with buffer solution of pH 4.5 were investigated using FESEM analysis (figure 2). The morphology of pure PVA [figure 2(a)] has shown smooth morphology with no cracks of inhomogeneity on the surface confirming the amorphous nature complementing to the powder XRD analysis [8]. Whereas the morphology of the MRPVA3 [figure 2(b)] has also shown smooth morphology with small crystalline phases spread inhomogeneously throughout the surface ascribing to the presence of MR within the PVA matrix [11].…”

“…The pure PVA and the untreated MR/PVA have resembled same XRD pattern revealing the predominant diffraction angle at 2Ɵ=19.8 o . This peak has a d spacing of 4.57, which corresponds to the crystalline PVA's (101) reflection plane [8,9]. This pattern represents the semi-crystalline nature of PVA ascribing to the strong interaction between the PVA chains through intermolecular and intramolecular hydrogen bonding [10].…”

Antibacterial hydrogel dressings and their applications in wound treatment

“…Several works describe the use of gamma irradiation to reduce silver ions present in a polymer matrix to simultaneously generate silver nanoparticles and hydrogels (Alcântara et al, 2020, Kumaraswamy et al, 2020, Khozemy et al, 2018, de Lima et al, 2018. For instance, Lima et al produced a hydrogel containing polyvinylpyrrolidone, polyethylene glycol, agar, and carboxymethyl cellulose with silver nanoparticles using Cobalt 60 gamma irradiation under an inert atmosphere (nitrogen).…”

Green Synthesis of Chitosan–Selenium Bionanocomposite with High Biocompatibility and Its Marked Impact on Las B and RhII Genes Expression in Pseudomonas aeruginosa

“…The biocompatibility of the diverse nanocomposites using various models has been reported. Ti3C2Tx (MXene) nanocomposite using zebra sh embryo model [54] silver polyvinyl hydrogel nanocomposite using melanoma cell lines [55] polyacrylate/ZnO nanocomposite [56] using activated sludge, passion fruit like nanoarchitecture using zebra sh mode [57] chitosan nanocomposite on zebra sh model [58]. In this present investigation, the biocompatibility of CS-SeNC was checked using the zebra sh model by determining oxidative stress enzymes, apoptosis genes expression pattern, and liver histopathological analysis.…”