<i>In vitro</i> biocompatibility study of EDC/NHS cross-linked silk fibroin scaffold with olfactory ensheathing cells

Li, Shengwen; Wu, Peng; Ji, Zhongqing; Zhang, Yu; Azhari, Azhari; He, Yongqing; Shen, Yixin

doi:10.1080/09205063.2022.2135076

Cited by 14 publications

(5 citation statements)

References 46 publications

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“…The positive charge of quaternary ammonium groups on the surface of PET‐g‐PAA@As will interact with the negative charge of cytoderm by electrostatic interactions. Quaternary ammonium groups can penetrate the cell membrane and improve permeability to kill bacteria 34 . It was confirmed that As exhibited excellent antibacterial effect against S. aureus and E. coil through qualitative and quantitative tests.…”

Section: Resultsmentioning

confidence: 80%

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Amino polysaccharide quaternary ammonium salt grafted to PET‐Cuff by ultraviolet irradiation for improving the sustained antibacterial property of semi‐permanent central vein tunneled cuffed catheter

Liu,

Li,

Shi

et al. 2024

Polymers for Advanced Techs

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In hemodialysis, tunneled cuffed catheter (TCC) is commonly used as a medical device for establishing vascular access. To address the current problem that the skin tunnel of TCC is susceptible to bacterial infection, this work proposes quaternized modification of the cuff of TCC to comprehensively enhance the long‐lasting antimicrobial performance of TCC. The morphology of the cuff basically resembles the polyethylene terephthalate (PET). Via ultraviolet (UV) irradiation, acrylic acid (AA) was grafted onto PET. Then, amino polysaccharide quaternary ammonium salt (As) replaced H+ of the carboxyl groups on the macromolecular chain of PET through solution post‐treatment method. Through the single factor experiment, the degree of grafting reached 159.8% under the optimum preparation conditions (AA concentration of 25 vol%, H+ concentration of 0.2 mol/L, photosensitizer capacity of 15 wt%, and photo‐irradiation time of 30 min). By the above method, we successfully synthesized a highly hydrophilic, well biocompatible, and highly efficient antimicrobial PET‐g‐PAA@As. Animal experiments and histological analyses showed that PET‐g‐PAA@As was superior to PET control and blank control groups in controlling infection and promoting wound healing. This catheter material with excellent biocompatibility, sustained antimicrobial activity, and wound healing ability will have great potential for use in hemodialysis.

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Section: Resultsmentioning

confidence: 80%

“…Quaternary ammonium groups can penetrate the cell membrane and improve permeability to kill bacteria. 34 It was confirmed that As exhibited excellent antibacterial effect against S. aureus and E. coil through qualitative and quantitative tests.…”

Section: Analysis Of Antibacterial Activitiesmentioning

confidence: 78%

Amino polysaccharide quaternary ammonium salt grafted to PET‐Cuff by ultraviolet irradiation for improving the sustained antibacterial property of semi‐permanent central vein tunneled cuffed catheter

Liu,

Li,

Shi

et al. 2024

Polymers for Advanced Techs

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“…17 Cross-linking strategies using carbodiimides such as 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) exhibit a good biological response because they are water-soluble zero-length crosslinkers. 30,31 Unlike other crosslinker agents such as glutaraldehyde, EDC allows the formation of stable covalent bonds without becoming part of the crosslinked gelatin network, which can avoid the cytotoxic effect. 26 EDC can be used for coupling polymers containing carboxyl groups and amines.…”

Section: Introductionmentioning

confidence: 99%

Physical Characterization of Crosslinked Gelatin Hydrogel as a Potential Biomaterial for Gingival Tissue Regeneration

Hutomo,

Deandra,

Ketherin

et al. 2024

Preprint

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The development of biomaterial as substitution to connective tissue graft for treating gingival recession is growing nowadays. Hydrogels received attention as potential scaffold to be used in gingival tissue engineering since their capability to mimic soft tissue characteristic as they can hold amount of water. Gelatin is a natural polymer that widely used in hydrogel preparation with good biological properties, but it has poor physical stability for the used as scaffold. Chemical crosslinking strategy using EDC/NHS was performed in this study to overcome its limitation. One uncrosslinked gelatin and two crosslinked gelatin hydrogels different concentration of EDC/NHS were prepared in this study. This study investigated the difference of physical characterization from the prepared gelatin hydrogels. Our findings suggest the crosslinking strategy using EDC/NHS increase physical properties of the gelatin hydrogels without interfering the chemical structure of the gelatin. The higher crosslinker concentration will result in better morphology and more resilient to biodegradation. As a result, the crosslinked gelatin hydrogel with gelatin:EDC:NHS mass ratio of 12:1:1 met the physical characteristic for future use as scaffold for gingival tissue regeneration.

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“…EDC/NHS-induced crosslinking involves a condensation reaction without the introduction of a chemical reagent. This type of crosslinking, known as "zero-length" crosslinking, is generally considered to be biocompatible and non-toxic [49]. EDC/NHS has also been explored as a potential alternative to glutaraldehyde.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Assessment of Collagen/Sodium Alginate-Based Sponges as Hemostatic Dressings

Sun,

Shen,

et al. 2024

Molecules

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In our search for a biocompatible composite hemostatic dressing, we focused on the design of a novel biomaterial composed of two natural biological components, collagen and sodium alginate (SA), cross-linked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) and oxidized sodium alginate (OSA). We conducted a series of tests to evaluate the physicochemical properties, acute systemic toxicity, skin irritation, intradermal reaction, sensitization, cytotoxicity, and in vivo femoral artery hemorrhage model. The results demonstrated the excellent biocompatibility of the collagen/sodium alginate (C/SA)-based dressings before and after crosslinking. Specifically, the femoral artery hemorrhage model revealed a significantly shortened hemostasis time of 132.5 ± 12.82 s for the EDC/NHS cross-linked dressings compared to the gauze in the blank group (hemostasis time of 251.43 ± 10.69 s). These findings indicated that C/SA-based dressings exhibited both good biocompatibility and a significant hemostatic effect, making them suitable for biomedical applications.

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In vitro biocompatibility study of EDC/NHS cross-linked silk fibroin scaffold with olfactory ensheathing cells

Cited by 14 publications

References 46 publications

Amino polysaccharide quaternary ammonium salt grafted to PET‐Cuff by ultraviolet irradiation for improving the sustained antibacterial property of semi‐permanent central vein tunneled cuffed catheter

Amino polysaccharide quaternary ammonium salt grafted to PET‐Cuff by ultraviolet irradiation for improving the sustained antibacterial property of semi‐permanent central vein tunneled cuffed catheter

Physical Characterization of Crosslinked Gelatin Hydrogel as a Potential Biomaterial for Gingival Tissue Regeneration

Comprehensive Assessment of Collagen/Sodium Alginate-Based Sponges as Hemostatic Dressings

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