In vitro biofilm formation on the surface of resin‐based dentine adhesives

Abstract

Prevention of root caries on exposed root surfaces in the aging population is a significant challenge. Bonding resins can be applied to exposed root surfaces as sealants; however, minimal data exists regarding biofilm formation on the surface of these resins. We hypothesized that an antibacterial dentine-bonding resin containing methacryloxydodecyl-pyridiniumbromide (MDPB) may reduce biofilm formation. Biofilms were produced in pooled stimulated natural whole saliva, supplemented with 1% sucrose, on the surface of 5 dentine-bonding resins (Clearfil SE, OptiBond Solo, Protect Bond, Protect Bond Primer, and Xeno III) using untreated root surfaces as controls. Biofilms were stained using the Live:Dead Baclight bacterial viability stain, viewed with confocal microscopy, and analyzed using ImageJ image-analysis software. Resin surfaces encouraged attachment of live bacteria compared with root surfaces. All resins showed similar bacterial colonization in sections adjacent to the resin surface, but in the central and outer portions of biofilms, Xeno III and Protect Bond Primer showed a viable bacterial load similar to that of the root surface. Fluoride-releasing resins (OptiBond Solo/Protect Bond) did not show reduced biofilm formation. Thus, antibacterial agents within the resins have a minimal effect on biofilm formation, particularly when directly adjacent to the root surface.

In vitro analysis of inhibitory effects of the antibacterial monomer MDPB-containing restorations on the progression of secondary root caries

“…Possibly the amount of adhesive system exposed in this thin line at the tooth-restoration interface would not be enough to release fluoride in the amount able to inhibit the biofilm formation and acid production activity. In fact, Rolland et al, 44 testing the antibacterial effects of resin-based adhesives as root surfaces sealants, did not found reduction in the biofilm formation when fluoride-releasing bonding resins were used.…”

Mineral content of ionomer cements and preventive effect of these cements against white spot lesions around restorations

“…In addition, under acidic conditions, a much greater number of ions are released from the glass-ionomer cement, and the high release rates are sustained. 23,24 This dynamic is crucial for caries prevention.…”

Effects of CO2laser irradiation on matrix-rich biofilm development formation–an in vitro study

“…Thus, live bacteria with intact green membranes fluoresce, while dead bacteria with damaged membranes fluoresce red were evidenced. Fluorescent images of the double staining were captured using fluorescence microscopy (EVOS fl microscope AMG; Bothell, WA, USA) (Rolland et al, 2006). …”