In vitro biological activities of lotus (Nelumbo nucifera) leaves extract

Abstract

Antioxidative, anticoagulant, and α-glucosidase inhibitory effects of 60% (v/v) ethanolic extract from lotus (Nelumbo nucifera) leaves were investigated in vitro. The yield and polyphenol content of the lotus leaves extract were 35.3±1.1% and 186.2±5.2 μg gallic acid equivalents/mg, respectively. The lotus leaves extract effectively scavenged free and cation radicals, and nitrite in a concentration-dependent manner, and possessed potent reducing power. The lotus leaves extract had remarkable anticoagulant and α-glucosidase inhibitory activities as a function of the extract concentration.

Biological activities of ethanolic extract from Robinia pseudoacacia L. flower

“…The IC 50 values of BLFE, which is the concentration at which 50% of DPPH or ABTS radicals were scavenged, were 2,920.2 and 961.4 μg/mL, respectively. The IC 50 value for DPPH radical scavenging of BLFE was higher than 205.1 μg/mL of lotus leaf extract [15], 297.1 μg/mL of mugwort extract [16] and 574.2 μg/mL of Du-zhong leaf extract [17], and was similar to 2,103.1 μg/mL of Du-zhong bark extract [17]. DPPH free radical scavenging ability of each plant extract was proportional to the polyphenol contents.…”

“…DPPH free radical scavenging ability of each plant extract was proportional to the polyphenol contents. The IC 50 value for ABTS radical scavenging of BLFE was higher than 274.1 μg/mL of lotus leaf extract [15], 226.1 μg/mL of mugwort extract [16] and 560.6 μg/mL of Du-zhong leaf extract [17], and was lower than 1,357.4 μg/mL of Du-zhong bark extract [17] and 4,762.8 μg/mL of ginseng root extract [18]. The BLFE had better scavenging ability against ABTS cation radicals than that against DPPH free radicals.…”

“…The polyphenol content of 40% (v/v) ethanolic extract from BLF was 39.8±0.5 mg gallic acid equivalent (GAE)/g-extract (Table 1). When the polyphenol content of BLFE was compared with that of extracts from other medicinal plants, it was lower than 186.2 mg/g [15] of lotus (Nelumbo nucifera) leaf, 106.9 mg/g of mugwort (Artemisia princeps Pampanini) [16] and 64.1 mg/g of Du-zhong (Eucommia ulmoides Oliver) leaf [17], but it was higher than 10.3 mg/g of ginseng (Panax ginseng) root [18] and 10.4 mg/g of notoginseng (Panax notoginseng) root [18]. The polyphenol content varies greatly from plant type as well as from parts and organs within the same plant [19,20].…”

Antioxidant potential of root extracts of Panax ginseng and Panax notoginseng

“…식물에서 항산화 활성을 비롯한 다양한 생리활성의 근원이 되는 대표적 인 물질로 폴리페놀 화합물이 널리 알려져 있다 [18]. 인삼과 전 칠삼 추출물의 폴리페놀 함량은 모시잎 추출물의 105.0 mg/g [19], 두충잎 추출물의 64.1 mg/g [20], 맥문동 잎 추출물의 174.2 mg/g [21], 연잎 추출물의 186.2 mg/g [22] 등 식물의 잎 추출 물보다는 크게 낮았으나, 와송 뿌리 추출물의 1.7 mg/g [23], 맥 문동 뿌리 추출물의 3.9 mg/g [21] 등 다른 식물의 뿌리 추출 물에 비해서는 높게 나타났다. 일반적으로 식물의 생리활성 성 분과 그 함량은 부위에 따라 차이가 크게 있는데 [24], 여러 약 용식물을 부위별로 추출하여 폴리페놀 함량을 비교한 결과에서 도 뿌리에서 낮고 잎과 껍질에서 높게 나타났다 [25].…”

“…또한 추출물의 폴 리페놀 함량은 추출용매의 극성에 따라 달라지는데 전칠삼의 경 우 물 추출물에서는 2.8 mg/g [13], 에탄올 추출물에서는 8.58 mg/g [26]로 나타나 본 연구의 전칠삼의 30% 에탄올 추출물의 폴리페놀 함량(10.4 mg/g)이 더 높았다. 본 연구에서 인삼과 전 칠삼 모두 30% (v/v) 에탄올 수용액 추출 시 폴리페놀 함량이 가장 높게 나타났는데, 이는 식물체를 물이나 에탄올로 추출할 때보다 두 용매가 혼합된 에탄올 수용액으로 추출 시 극성과 물인 두충잎 추출물 2.33 mg/mL [20], 연잎 추출물 0.58 mg/mL [22], 약쑥 추출물 0.98 mg/mL [29]…”